AUTHOR=Meena Dharm Singh , Sharma Lata , Bishnoi Jyoti , Soni Monika , Jeph Nirmal Kumar , Galav Vikas , Sharma Sandeep Kumar 

TITLE=Serological and molecular prevalence of Brucella spp. among livestock species in Rajasthan, India

JOURNAL=Frontiers in Veterinary Science

VOLUME=Volume 10 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1157211

DOI=10.3389/fvets.2023.1157211

ISSN=2297-1769

ABSTRACT=A seroprevalence and molecular study was carried out in six districts of Rajasthan State, India to detect Brucellosis brucellosis in major livestock species. This study involved the testing of 3,245 livestock samples using the Rose BengalPlate Test (RBPT), Indirect Enzyme-Linked Immunosorbent Assay (i-ELISA) and genus-specificpolymerase chain reaction (PCR) markers for molecular diagnosis of the disease. In the tested samples seroprevalence was 5.06% (CI: 1.96-8.15) using the RBPT test and 6.88% (CI: 1.98-11.78) using the i-ELISA test while the cumulative seroprevalence (RBPT and i-ELISA) was 3.63% (CI: 0.44-6.83). The prevalence of the disease was 1.27% (CI: -0.56-3.11) when tested using the molecular markers.The highest prevalence of Brucellosis brucellosis was detected in Cattle (7.00%, 3.22%) followed by camel (5.50%, 2.50%), buffalo (2.66%, 0.00%),sheep (2.43%, 0.41%), and goat (0.58%, 0.23%) when serological (cumulative) and molecular diagnosis are considered as preferred methods of detection. Cattle (3.22%) and camels (2.50%) also showed high prevalence of disease when tested using molecular markers. The results of this study revealed that cattle, camel and sheep brucellosis is prevalent in the study areas.