AUTHOR=Chen Xing , Zhang Guang-min , Wang Wei-wei , Liu Guo-hua , Cai Hui-yi , Purba Adanan , Zheng Ai-juan TITLE=Compound non-starch polysaccharide enzymes improve growth performance, slaughter performance, immune function, and apparent utilization rate of nutrients in broiler chickens fed a low-metabolizable energy diet JOURNAL=Frontiers in Veterinary Science VOLUME=Volume 10 - 2023 YEAR=2023 URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1162811 DOI=10.3389/fvets.2023.1162811 ISSN=2297-1769 ABSTRACT=This study aimed to investigate the compound non-starch polysaccharide (NSP) enzymes on growth performance, slaughter performance, immune function, and apparent nutrient utilization of broiler chickens fed with a low metabolizable energy diet. A total of 240 healthy 1-day-old AA broilers (Arbor Acres, 47.2 ± 0.31g) were randomly divided into four treatments of six replicates, with 10 broilers per replicate. The control group was the basal diet, and the EL-H group was the basal diet supplemented 200 mg/kg compound NSP enzyme, including β-mannanase 5000 IU/g, β-glucanase 2000 IU/g, xylanase 10000 IU/g, and cellulase 500 IU/g. EL-M group was the basal diet supplemented with 200 mg/kg compound NSP enzyme after 50 kcal/kg metabolizable energy was removed from the basic diet. The EL-L group was the basal diet supplemented with 200 mg/kg compound NSP enzyme after 100 kcal/kg metabolizable energy was removed from the basic diet. The results showed that compound NSP enzymes supplementing in low metabolizable energy diets did not significantly affect on growth performance of broilers (P > 0.05). Compared with the control group, the abdominal fat rate of broilers in the EL-L group was significantly decreased, and that of the EL-M group was significantly increased (P < 0.05). In the control group, the apparent utilization of dry matter, crude protein, and energy in the diet was lower than that of the EL-L group while significantly higher than that of the EL-H group (P < 0.05). In addition, the apparent utilization of crude fiber in EL-H, EL-M, and EL-L groups was significantly increased when compared with the control group (P < 0.05). In conclusion, this experiment showed that adding 200 mg/kg compound NSP enzyme maintained the normal growth and development of broiler chickens fed the low metabolizable energy diet (replacing 50-100 kcal/kg metabolizable energy). This study provides a theoretical basis for the application of the compound NSP enzyme in broiler chickens.