ORIGINAL RESEARCH article

Front. Vet. Sci., 02 February 2024

Sec. Parasitology

Volume 10 - 2023 | https://doi.org/10.3389/fvets.2023.1324619

Chewing lice of wild birds in Iran: new data and a checklist of avian louse species reported in Iran

  • 1. Department of Pathobiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran

  • 2. Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University, Mashhad, Iran

  • 3. Department of Clinical Sciences, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran

  • 4. Provincial Department of Environment, Hamedan, Iran

  • 5. Department of Parasitology, Veterinary Faculty, Selçuk University, Konya, Türkiye

Abstract

Between September 2019 and December 2023, a total of 612 wild birds representing 16 orders, 33 families, 60 genera, and 78 species from nine provinces of Iran with different climates namely Hamedan (n = 54), Sistan-va-Baluchestan (n = 372), Kerman (n = 73), South Khorasan (n = 52), Mazandaran (n = 7), Chaharmahal-va-Bakhtiari (n = 2), Gilan (n = 2), Golestan (n = 18), North Khorasan (n = 9), and Razavi Khorasan (n = 23) were examined for chewing lice infestation. Naked eye examination revealed that 58 birds (9.5%) were infested with at least one chewing louse species. Collected lice specimens belonged to 28 species from the families Philopteridae, Menoponidae and Laemobothriidae including Strigiphilus strigis (n = 55, 15.6%), Falcolipeurus quadripustulatus (n = 41, 11.6%), Craspedorrhynchus platystomus (n = 40, 11.3%), Colpocephalum turbinatum (n = 36, 10.2%), Laemobothrion maximum (n = 25, 7.1%), Nosopon lucidum (n = 20, 5.6%), Degeeriella fulva (n = 18, 5.1%), Colpocephalum eucarenum (n = 16, 4.5%), Laemobothrion vulturis (n = 15, 4.2%), Anaticola crassicornis (n = 13, 3.7%), Craspedorrhynchus aquilinus (n = 9, 2.5%), Degeeriella fusca (n = 7, 2.0%), Aegypoecus trigonoceps (n = 7, 2.0%), Quadraceps obscurus (n = 6, 1.7%), Colpocephalum impressum (n = 6, 1.7%), Trinoton querquedulae (n = 6, 1.7%), Colpocephalum heterosoma (n = 5, 1.4%), Colpocephalum nanum (n = 5, 1.4%), Lunaceps holophaeus (n = 4, 1.1%), Quadraceps spp. (n = 4, 1.1%), Actornithophilus uniseriatus (n = 2, 0.6%), Nosopon chanabense (n = 2, 0.6%), Actornithophilus cornutus (n = 1, 0.3%), Cuclotogaster heterographus (n = 1, 0.3%), Falcolipeurus suturalis (n = 1, 0.3%), Laemobothrion atrum (n = 1, 0.3%), Colpocephalum gypsi (n = 1, 0.3%), and Rallicola cuspidatus (n = 1, 0.3%). All of these species except six, i.e., Trinoton spp., C. aquilinus, L. vulturis, L. maximum, C. impressum, C. turbinatum, and C. heterographus are recorded for the first time from Iran. This study is the largest epidemiological study to date performed in the country. Data reported herein contribute to our knowledge about diversity of avian chewing lice from wild birds in Iran. In this paper, an updated checklist of louse species reported from Iran according to their avian hosts is presented.

Introduction

Lice are small (0.35–11 mm long as adults), wingless, dorsoventrally flattened insects. They are obligatory, permanent ectoparasites of birds and mammals throughout the world which typically, parasitize individuals in small numbers and cause no apparent discomfort however, some of the lice can cause skin lesions and act as vectors or intermediate hosts of several bacteria, viruses and filarial parasites (1, 2). In addition, it has been shown that Piagetiella titan infesting white pelicans may invade the oral cavity causing erosions and petechial hemorrhages (3–5).

Lice (Insecta: Psocoptera: Phthiraptera) with about 5,000 known species, present on roughly 4,000 species of birds and 800 mammals, are categorized in four suborders (6). Species of the suborder Anoplura have adopted to suck blood from capillaries of mammals and ingest it, while Amblycera, Ischnocera, and Rhynchophthirina (formerly known as Mallophaga) have chewing mouth pieces, adapted to eat hairs and feathers, and sometimes also the skin and blood of birds and mammals (7). Avian chewing lice belong to one of two sub-orders: Amblycera, which occur on feathers and skin, or Ischnocera, which are more restricted to feathers (1). Most of the lice species are strongly associated with hosts, their phylogeny parallels that of hosts, sometimes with different speeds however, “host specificity” cannot be assumed (7, 8). Among different fields of wildlife parasitology, studying avian chewing lice is important as their epizootiology is largely associated with geographical distribution of their hosts.

Iran is a country in western Asia with a territory of 1,648,195 km2. It is the second largest country in the Middle East and the 17th largest in the world. In the country, 550 avian species are distributed which is almost equal to the richness of birds in Europe (9, 10). However, there is limited and scanty information about their parasites fauna specially the chewing lice (11–14) with several published in Persian language and presented in local congresses (15–19). Considering the scarcity of published records of lice in Iran, we aimed to gather new data and present an updated checklist of birds’ Phthiraptera occurring in the country.

Materials and methods

Between September 2019 and December 2023, totally 612 wild birds belonging 16 orders, 33 families, 60 genera, and 78 species from Hamedan (n = 54), nine different regions of Sistan-va-Baluchestan (n = 372), Kerman (n = 73), South Khorasan (n = 52), Mazandaran (n = 7), Chaharmahal-va-Bakhtiari (n = 2), Gilan (n = 2), Golestan (n = 18), North Khorasan (n = 9), and Razavi Khorasan (n = 23) were collected (Figure 1). The birds were euthanized by the Provincial Department of Environment because of general health failure or were found dead in the environment. The time lapse from death to examination of birds for lice infestation could not be estimated however, only fresh carcasses were examined. Individual birds were sent to Laboratory of Parasitology, Faculty of Veterinary Medicine, Bu-Ali Sina University in sealed plastic bags for examination or were examined in the field. The bird identifications were made using the reference book Atlas of Birds of Iran (9), and a standard examination for searching chewing lice was performed (20). The collected lice were placed in tubes containing 70% ethanol, cleared in 10% KOH for at least 1 day, mounted in Canada balsam on glass slides (21), and identified according to the original descriptions or keys (7, 22–35) using a Leica DM750 camera mounted trinocular microscope with Leica DFC295 application unit.

Figure 1

We also collected all the available information about chewing lice infesting birds in Iran. The databases and search engines employed for the literature review were Phthiraptera.info,1 PubMed,2 Google,3 Scientific Information Database of Iran,4 the collection of defended theses at all Iranian Universities,5 and the collection of proceedings of Iranian congresses.6 Valid names of the louse and bird species were obtained from Global Biodiversity Information Facility resources (36).

Results

In total, 352 lice specimens including Strigiphilus strigis n = 55; 15.6% (Pontoppidan, 1763), Falcolipeurus quadripustulatus n = 41; 11.6% (Burmeister, 1838), Craspedorrhynchus platystomus n = 40; 11.3% (Burmeister, 1838), Colpocephalum turbinatum n = 36; 10.2% (Denny, 1842), Laemobothrion maximum n = 25; 7.1% (Scopoli, 1763), Nosopon lucidum n = 20; 5.6% (Rudow, 1869), Degeeriella fulva n = 18; 5.1% (Giebel, 1874), Colpocephalum eucarenum n = 16; 4.5% (Burmeister, 1838), Laemobothrion vulturis n = 15; 4.2% (Fabricius, 1775), Anaticola crassicornis n = 13; 3.7% (Scopoli, 1763), Craspedorrhynchus aquilinus n = 9; 2.5% (Denny, 1842), Degeeriella fusca n = 7; 2.0% (Denny, 1842), Aegypoecus trigonoceps n = 7; 2.0% (Giebel, 1874), Quadraceps obscurus n = 6; 1.7% (Burmeister, 1838), Colpocephalum impressum n = 6; 1.7% (Rudow, 1866), Trinoton querquedulae n = 6; 1.7% (Linnaeus, 1758), Colpocephalum heterosoma n = 5; 1.4% (Clay, 1951), Colpocephalum nanum n = 5; 1.4% (Piaget, 1890), Lunaceps holophaeus n = 4; 1.1% Burmeister, 1838, Quadraceps spp. (new species) n = 4; 1.1% (Clay and Meinertzhagen, 1939), Actornithophilus uniseriatus n = 2; 0.6% (Piaget, 1880), Nosopon chanabense n = 2; 0.6% (Ansari, 1951), Actornithophilus cornutus n = 1; 0.3% (Giebel, 1866), Cuclotogaster heterographus n = 1; 0.28% (Nitzsch, 1866), Falcolipeurus suturalis n = 1; 0.3% (Rudow, 1869), Laemobothrion atrum n = 1; 0.3% (Nitzsch, 1818), Colpocephalum gypsi n = 1; 0.3% (Eichler & Zlotorzycka, 1971), and Rallicola cuspidatus n = 1; 0.3% (Scopoli, 1763) were collected from 58/612 birds (9.5%). Collected lice specimens belonged to 31 species from the families Philopteridae, Menoponidae, and Laemobothriidae. All of the identified lice species except C. aquilinus, L. vulturis, L. maximum, C. impressum, C. turbinatum, and C. heterographus are recorded for the first time from Iran (Table 1).

Table 1

Host informationParasite information
n birds examinedHost bird taxonomyHost scientific nameHost vernacular nameLouse speciesSuborderFamilyCityn total/infestedLouse prevalence (n)
MaleFemaleNymphDamagedTotal
ACCIPITRIFORMES
Accipitridae
1Accipiter badius (Gmelin, 1788)Shikra---Zahedan0/1
8Accipiter nisus (Linnaeus, 1758)Eurasian sparrowhawk---Hamun0/2
Hamedan0/5
Zabol0/1
1Aegypius monachus (Linnaeus, 1758)Cinereous vulture---Zahedan0/1
4Aquila chrysaetos (Linnaeus, 1758)Golden eagleCraspedorrhynchus aquilinus (Denny, 1842)IschnoceraPhilopteridaeHamedan3/354009
Kerman0/1
1Aquila heliaca (Savigny, 1809)Asian imperial eagleLaemobothrion maximum (Scopoli, 1763)AmblyceraLaemobothriidaeHamedan1/100303
3Aquila nipalensis (Hodgson, 1833)Steppe eagleLaemobothrion maximum (Scopoli, 1763)AmblyceraLaemobothriidaeHamedan2/324006
Laemobothrion vulturis (Fabricius, 1775)1/302204
Colpocephalum impressum Rudow, 1866Menoponidae1/301001
Craspedorrhynchus aquilinus (Denny, 1842)IschnoceraPhilopteridae1/301001
Falcolipeurus suturalis (Rudow, 1869)1/301001
1Aquila rapax (Temminck, 1828)Tawny eagleLaemobothrion vulturis (Fabricius, 1775)AmblyceraLaemobothriidaeHamedan1/110001
Colpocephalum impressum (Rudow, 1866)Menoponidae1/140105
Nosopon chanabense (Ansari, 1951)1/102002
14Buteo buteo (Linnaeus, 1758)BuzzardDegeeriella fulva (Giebel, 1874)IschnoceraPhilopteridaeHamedan1/141080018
Degeeriella fusca (Denny, 1842)1/1424107
Cuclotogaster heterographus (Nitzsch, 1866)1/1410001
Craspedorrhynchus platystomus (Burmeister, 1838)2/1418193040
Colpocephalum nanum (Piaget, 1890)AmblyceraMenoponidae1/1402215
Colpocephalum turbinatum (Denny, 1842)1/1424120036
Laemobothrion maximum (Scopoli, 1763)LaemobothriidaeKerman1/12336012
3Buteo rufinus (Cretzschmar, 1829)The long-legged buzzardLaemobothrion maximum (Scopoli, 1763)AmblyceraLaemobothriidaeKerman1/111103
Zabol0/1
Zahedan0/1
3Circus aeruginosus (Linnaeus, 1758)Eurasian marsh-harrierNosopon lucidum (Rudow, 1869)AmblyceraMenoponidaeHamedan1/36122020
2Circaetus gallicus (Gmelin, 1788)Short-toed snake eagle---Zahedan0/2
4Gyps fulvus (Hablizl, 1783)Griffon vultureLaemobothrion vulturis (Fabricius, 1775)AmblyceraLaemobothriidaeHamedan2/4154010
Colpocephalum gypsi (Eichler & Zlotorzycka, 1971)MenoponidaeZabol1/410001
Colpocephalum spp.1/400011
Falcolipeurus quadripustulatus (Burmeister, 1838)IschnoceraPhilopteridae2/423180041
Aegypoecus trigonoceps (Giebel, 1874)Kerman1/433107
ANSERIFORMES
Anatidae
6Aythya ferina (Linnaeus, 1758)Common pochard---Zehak0/3
Zabol0/1
Chabahar0/2
10Anas crecca (Linnaeus, 1758)Common TealTrinoton querquedulae (Linnaeus, 1758)AmblyceraMenoponidaeZehak5/803003
Anaticola crassicornis (Scopoli, 1763)IschnoceraPhilopteridaeChabahar0/235109
7Anas platyrhynchos (Linnaeus, 1758)MallardAnaticola crassicornis (Scopoli, 1763)IschnoceraPhilopteridaeZehak0/131004
Zabol2/2
Trinoton querquedulae (Linnaeus, 1758)AmblyceraMenoponidaeHamedan1/101001
Chabahar0/3
1Anas penelope (Linnaeus, 1758)Eurasian wigeonLaemobothrion spp.AmblyceraLaemobothriidaeZabol1/100101
9Spatula clypeata (Linnaeus, 1758)Northern shovelerPectinopygus spp.IschnoceraPhilopteridaeZabol1/301001
Zehak
Chabahar
1Mergus merganser (Linnaeus, 1758)Common merganser---Zabol0/1
2Spatula querquedula (Linnaeus, 1758)GarganeyTrinoton querquedulae (Linnaeus, 1758)AmblyceraMenoponidaeZehak1/210001
2Tadorna tadorna (Linnaeus, 1758)Common shelduck---Zehak0/2
BUCEROTIFORMES
3UpupidaeUpupa epops (Linnaeus, 1758)Eurasian hoopoe---Nehbandan0/1
Birjand0/1
Zabol0/1
CHARADRIIFORMES
Recurvirostridae
4Himantopus himantopus (Linnaeus, 1758)Black-winged stiltActornithophilus uniseriatus (Piaget, 1880)AmblyceraMenoponidaeZehak4/410102
Quadraceps spp.IschnoceraPhilopteridae30104
Scolopacidae
3Philomachus pugnax (Linnaeus, 1758)RuffLunaceps holophaeus (Burmeister, 1838)MallophagaPhilopteridaeZehak3/322004
Actornithophilus cornutus (Giebel, 1866)AmblyceraMenoponidae10001
2Tringa stagnatilis (Bechstein, 1803)Marsh sandpiperQuadraceps obscurus (Burmeister, 1838)IschnoceraPhilopteridaeZehak2/233006
14Phalaropus lobatus (Linnaeus, 1758)Red-necked phalarope---Zehak0/14
Laridae
17Sterna repressa (Hartert, 1916)White-cheeked tern---Zehak0/7
Zabol0/4
Hamun0/6
CAPRIMULGIFORMES
Caprimulgidae
8Caprimulgus aegyptius (Lichtenstein, 1823)Egyptian nightjar---Hamun0/3
Zabol0/1
Nimruz0/2
Zehak0/2
COLUMBIFORMES
Columbidae
22Streptopelia decaocto (Frivaldszky, 1838)Eurasian collared dove---Hirmand0/4
Zabol0/4
Nimruz0/3
Zehak0/6
Hamun0/5
36Spilopelia senegalensis (Linnaeus, 1766)Laughing dove---Hirmand0/1
Zabol0/8
Nimruz0/2
Zehak0/5
Sari0/1
Kerman0/2
Zahedan0/2
Mashhad0/3
Kalaleh0/2
Gorgan0/2
Nehbandan0/1
Birjand0/1
Hamun0/6
FALCONIFORMES
Falconidae
46Falco tinnunculus (Linnaeus, 1758)KestrelLaemobothrion maximum (Scopoli, 1763)AmblyceraLaemobothriidaeHamedan1/1200101
Kerman0/26
Zabol0/4
Zahedan0/4
4Falco cherrug (Gray, 1834)Saker falcon---Kerman0/4
1Falco naumanni (Fleischer, 1818)Lesser kestrel---Zabol0/1
2Falco peregrinus subsp. pelegrinoides (Temminck, 1829)Barbary falcon---Kerman0/2
GALLIFORMES
Phasianidae
22Ammoperdix griseogularis (Brandt, 1843)See-see partridge---Zahedan0/8
Nehbandan0/3
Iranshahr0/1
Khash0/3
Chabahar0/1
Birjand0/1
Qaen0/1
Torbat-Heidarie0/2
Gonaabaad0/1
Saravan0/1
11Alectoris chukar (Gray, 1830)Chukar---Iranshahr0/3
Saravan0/4
Chabahar0/1
Zahedan0/3
15Coturnix coturnix (Linnaeus, 1758)Common quail---Kerman0/14
Zabol0/1
5Francolinus francolinus (Linnaeus, 1766)Black francolin---Hirmand0/1
Zehak0/1
Hamun0/3
GRUIFORMES
Rallidae
1Rallus aquaticus (Linnaeus, 1758)Water railRallicola cuspidatus (Scopoli, 1763)IschnoceraPhilopteridaeHamedan1/110001
18Fulica atra (Linnaeus, 1758)CootLaemobothrion (Eulaemobothrion) atrum (Nitzsch, 1818)AmblyceraLaemobothriidaeZehak0/12Zabol1/301001Hamun0/2Nimruz0/1
OTIDIFORMES
Otididae
1Chlamydotis macqueenii (Gray, 1832)MacQueen’s bustard---Kerman0/1
PASSERIFORMES
4AcrocephalidaeAcrocephalus scirpaceus (Hermann, 1804)Eurasian reed warbler---Zehak0/2
Zabol0/2
Alaudidae
5Alaemon alaudipes (Desfontaines, 1789)Greater hoopoe-larkHirmand0/2
Zabol0/2
Zehak0/1
11Alauda arvensis (Linnaeus, 1758)Eurasian skylark---Hamun0/2
Zehak0/1
Hirmand0/2
Zabol0/4
Nimruz0/2
35Galerida cristata (Linnaeus, 1758)Crested lark---Hamun0/4
Zabol0/4
Hirmand0/4
Birjand0/2
Neishaboor0/1
Quchan0/1
Torbat-Heidarie0/1
Gonaabaad0/1
Gorgan0/2
Shirvan0/2
Bojnurd0/2
Sari0/2
Qaen0/1
Nimruz0/4
Zehak0/4
4Melanocorypha calandra (Linnaeus, 1766)Calandra lark---Mashhad0/4
Cisticolidae
7Prinia gracilis (Lichtenstein, 1823)Graceful prinia---Zehak0/3
Hamun0/2
Zabol0/2
Fringillidae
5Serinus pusillus (Pallas, 1811)Red-fronted serin---Kerman0/5
5Carduelis carduelis (Linnaeus, 1758)European goldfinch---Mashhad0/1
Birjand0/1
Gorgan0/1
Kalaleh0/1
Kerman0/1
11Rhodospiza obsoleta (Lichtenstein, 1823)Desert finch---Nehbandan0/2
Qaen0/3
Mashhad0/3
Bojnurd0/1
Birjand0/1
Torbat-Heidarieh0/1
Hirundinidae
7Hirundo rustica (Linnaeus, 1758)Barn swallowHirmand0/2
Hamun0/1
Zehak0/4
Passeridae
19Passer hispaniolensis (Temminck, 1820)Spanish sparrow---Nimruz0/3
Zabol0/5
Zehak0/6
Hamun0/2
Hirmand0/3
23Passer domesticus (Linnaeus, 1758)House sparrow---Shirvan0/3
Gorgan0/1
Kalaleh0/4
Aqqala0/4
Rasht0/2
Sari0/1
Babol0/3
Birjand0/3
Nehbandan0/1
Quchan0/1
30Passer montanus (Linnaeus, 1758)Eurasian tree sparrow---Hamun0/7
Zabol0/9
Hirmand0/3
Zehak0/5
Nimruz0/6
Pycnonotidae
21Pycnonotus leucotis (Gould, 1836)White-eared bulbul---Hamun0/4
Zabol0/6
Hirmand0/4
Zehak0/5
Nimruz0/2
Laniidae
4Lanius phoenicuroides (Schalow, 1875)Red-tailed shrike---Hirmand0/1
Hamun0/1
Zehak0/1
Zabol0/1
Leiothrichidae
14Turdoides caudata (Dumont, 1823)Common babbler---Hamun0/4
Zabol0/3
Hirmand0/2
Zehak0/3
Nimruz0/2
Motacillidae
11Motacilla alba (Linnaeus, 1758)White wagtail---Gorgan0/1
Quchan0/1
Bojnurd0/1
Neishaboor0/1
Mashhad0/1
Zabol0/1
Qaen0/1
Nehbandan0/1
Zehak0/2
Hamun0/1
Muscicapidae
7Oenanthe albonigra (Hume, 1872)Hume’s wheatear---Zehak0/2
Hamun0/1
Zabol0/4
1Cercotrichas galactotes (Temminck, 1820)Rufous-tailed scrub robin---Zabol0/1
Scotocercidae
5Scotocerca inquieta (Cretzschmar, 1830)Streaked scrub warbler---Zehak0/2
Hamun0/1
Zabol0/2
PELECANIFORMES
Ardeidae
6Ardea cinerea (Linnaeus, 1758)Gray heron---Hamedan0/1
Zehak0/5
5Ardea alba (Linnaeus, 1758)Great egretZehak0/3
Hamun0/2
1Botaurus stellaris (Linnaeus, 1758)Great Bittern (Eurasian bittern)---Hamedan0/1
2Ixobrychus minutus (Linnaeus, 1766)Little bittern---Zahedan0/2
Pelecanidae
4Pelecanus crispus (Bruch, 1832)Dalmatian pelicanColpocephalum eucarenum Burmeister, 1838AmblyceraMenoponidaeZehak0/2655016
Zabol1/2
PHOENICOPTERIFORMES
Phoenicopteridae
3Phoenicopterus ruber (Linnaeus, 1758)American flamingoColpocephalum heterosoma Piaget, 1880, small specimen (Clay, 1951)AmblyceraMenoponidaeZabol1/113004
Colpocephalum heterosoma Piaget, 1880, large specimenZehak0/210001
PODICIPEDIFORMES
Podicipedidae
9Podiceps cristatus (Linnaeus, 1758)Great crested grebe---Zehak0/6
Zabol0/3
PTEROCLIDIFORMES
Pteroclidae
1Pterocles orientalis (Linnaeus, 1758)Black-bellied Sandgrouse---Hamedan0/1
PICIFORMES
Picidae
1Dendrocopos syriacus (Hemprich & Ehrenberg, 1833)Syrian woodpecker---Hamedan0/1
SULIFORMES
Phalacrocoracidae
7Phalacrocorax carbo (Linnaeus, 1758)Great cormorant---Zabol0/2
Zehak0/2
Chabahar0/3
STRIGIFORMES
Strigidae
2Asio otus (Linnaeus, 1758)Long-eared owlStrigiphilus sp.IschnoceraPhilopteridaeHamedan1/201001
9Athene noctua (Scopoli, 1769)Little owl---Hamedan0/1
Kerman0/4
Shahrekord0/2
Khash0/2
8Bubo bubo (Linnaeus, 1758)Eagle owlStrigiphilus strigis (Pontoppidan, 1763)IschnoceraPhilopteridaeHamedan2/226290055
Zahedan0/1
Zabol0/1
Kerman0/4
1Otus scops (Linnaeus, 1758)European scops owl---Hamedan0/1
2Otus brucei (Hume, 1873)Pallid scops owl---Kerman0/2
Tytonidae
4Tyto alba (Scopoli, 1769)Barn owl---Hamedan0/1
Kerman0/2
Zahedan0/1
Total 61258157157352352

Distribution of louse species of wild birds in some regions of Iran (September 2019 and December 2023) according to their host bird species.

aaccording to International Union for Conservation of Nature (IUCN) Red List of Threatened Species (www.iucnredlist.org). Names of orders are capitalized, and names of families are showed in bold.

Number of lice specimens collected from examined birds ranged from 1 to 55, the latter was a Bubo bubo Linnaeus, 1758. Mixed lice infestation was found in 11 birds, i.e., in one Philomachus pugnax (Linnaeus, 1758), two Himantopus himantopus Linnaeus, 1758, two Anas crecca Linnaeus, 1758, one Aquila nipalensis Hodgson, 1833, one Aquila rapax Temminck, 1828, two Gyps fulvus Hablitz, 1783, and two Buteo buteo Linnaeus, 1758. Photomicrographs of examined lice specimens are presented in Figures 214.

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

Figure 10

Figure 11

Figure 12

Figure 13

Figure 14

Identification of few specimens could not be performed to species level including one damaged female Strigiphilus sp. collected from Asio otus Linnaeus, 1758, one female Pectinopygus spp. collected from Anas clypeata Linnaeus, 1758 which is an unusual host and possibly was a contamination, one Laemobothrion spp. nymph, and four Quadraceps spp. (Figures 10AC, 13B, 14D). In addition, nits collected from one Falco tinnunculus Linnaeus, 1758 could not be identified.

In Supplementary Table 1, the information in Table 1 in addition to world conservation status according to International Union for Conservation of Nature (IUCN) and names of birds in Persian language are presented.

Discussion

This study is the largest epidemiological study to date performed in Iran. However, low number of collected lice from birds could be due to the fact that most of the ectoparasites including lice leave dead hosts rather quickly. Data reported herein contribute to our knowledge about diversity of avian chewing lice from wild birds in Iran and in a broader context in western Asia. Lice species in this study belonged to both Ischnocera (15 species), Amblycera (14 species). We compiled our data and previous information about avian lice species in Iran in Table 2. So far, lice infestation of birds belonging to 16 orders, 33 families, 60 genera, and 78 species and subspecies has been recorded from Iran. In Supplementary Table 2, the information in Table 2 in addition to world conservation status and names of birds in Persian language are presented.

Table 2

Avian host scientific nameAvian host vernacular nameLice speciesReference
ACCIPITRIFORMES
Accipitridae
Aquila chrysaetos (Linnaeus, 1758)Golden eagleCraspedorrhynchus aquilinus (Denny, 1842)This study (37, 38)
Laemobothrion maximum (Scopoli, 1763)
Laemobothrion sp.
Aquila fasciata (Vieillot, 1822)Bonelli’s eagleLaemobothrion maximum (Scopoli, 1763)Reported the bird as Hieraaetus fasciatus (39)
Aquila heliaca (Savigny, 1809)Asian imperial eagleLaemobothrion maximum (Scopoli, 1763)This study
Aquila nipalensis (Hodgson, 1833)Steppe eagleColpocephalum impressum (Rudow, 1866)This study (40)
Falcolipeurus suturalis (Rudow, 1869)
Laemobothrion maximum (Scopoli, 1763)
Craspedorrhynchus sp.
Aquila rapax (Temminck, 1828)Tawny eagleLaemobothrion vulturis (Fabricius, 1775)This study
Colpocephalum impressum (Rudow, 1866)
Nosopon chanabense (Ansari, 1951)
Buteo buteo (Linnaeus, 1758)BuzzardDegeeriella fulva (Giebel, 1874)This study
Degeeriella fusca (Denny, 1842)
Cuclotogaster heterographus (Nitzsch, 1866)
Craspedorrhynchus platystomus (Burmeister, 1838)
Colpocephalum nanum (Piaget, 1890)
Colpocephalum turbinatum (Denny, 1842)
Laemobothrion maximum (Scopoli, 1763)
Buteo rufinus (Cretzschmar, 1829)Long-legged buzzardTrinoton sp.×This study (17)
Laemobothrion maximum (Scopoli, 1763)
Circus aeruginosus (Linnaeus, 1758)Eurasian marsh-harrierNosopon lucidum (Rudow, 1869)This study
Gyps fulvus (Hablizl, 1783)Eurasian griffon vultureTrinoton sp.×This study (16)
Laemobothrion vulturis (Fabricius, 1775)
Colpocephalum gypsi (Eichler & Zlotorzycka, 1971)
Colpocephalum spp.
Falcolipeurus quadripustulatus (Burmeister, 1838)
Aegypoecus trigonoceps (Giebel, 1874)
Neophron percnopterus (Linnaeus, 1758)Egyptian vultureLaemobothrion vulturis (Fabricius, 1775)(15)
ANSERIFORMES
Anatidae
Anas clypeata (Linnaeus, 1758)Northern shovelerAnaticola crassicornis (Scopoli, 1763)This study (38)
Pectinopygus spp.
Anas crecca (Linnaeus, 1758)Common TealTrinoton querquedulae (Linnaeus, 1758)This study
Anaticola crassicornis (Scopoli, 1763)
Anas platyrhynchos (Linnaeus, 1758)MallardLipeurus squalidus (Piaget, 1880)×This study (41, 42)
Menacanthus stramineus (Nitzsch, 1818)×
Trinoton anserinum (Fabricius, 1805)×
Anatolica crassicornis (Scopoli, 1763)
Anas penelope Linnaeus, 1758Eurasian wigeonLaemobothrion spp.This study
Anas querquedula (Linnaeus, 1758)GarganeyTrinoton querquedualea (Linnaeus, 1758)This study
Anser anser (Linnaeus, 1758)Greylag gooseAnaticola anseris (Linnaeus, 1758)(15, 41, 43)
Trinoton anserinum (Fabricius, 1805)
Cuclotogaster heterographus (Nitzsch, 1866) ×. Also reported as Liperus heterographus×
Lipeurus caponis (Linnaeus, 1758)×
Menopon gallinae (Linnaeus, 1758)×
BUCEROTIFORMES
Upupidae
Upupa epops (Linnaeus, 1758)HoopoeUpupicola upupae (Schrank, 1803)(38)
CHARADRIIFORMES
Laridae
Chroicocephalus ridibundus (Linnaeus, 1766)Black-headed gullAustromenopon transversum (Denny, 1842)Reported the bird as Larus ridibundus (13)
Sterna hirundo (Linnaeus, 1758)TernQuadraceps legatus (Timmermann, 1952)This study (12)
Saemundssonia meridiana (Timmermann, 1950)This study
Tringa stagnatilis (Bechstein, 1803)Marsh sandpiperQuadraceps obscurus (Burm, 1838)This study
Recurvirostridae
Himantopus himantopus (Linnaeus, 1758)Black-winged stiltActornithophilus uniseriatus (Piaget, 1880)
Quadraceps spp.
This study
Scolopacidae
Philomachus pugnax (Linnaeus, 1758)RuffLunaceps holophaeus Burmeister, 1838This study
Actornithophilus cornutus (Giebel, 1866)This study
COLUMBIFORMES
Columbidae
Columba livia subsp. domestica (Gmelin, 1789)Domestic pigeonCampanulotes compar (Burmeister, 1838). Also reported as Goniocotes bidentatus(41, 44–46)
Columbicola columbae (Linnaeus, 1758)
Columbicola tschulyschman (Eichler, 1942)
Lipeurus caponis (Linnaeus, 1758)×
Menopon gallinae (Linnaeus, 1758)×
Menacanthus stramineus (Nitzsch, 1818)×. Also reported as Menopon stramineum×
Columba livia subsp. livia (Gmelin, 1789)Rock doveCampanulotes compar (Burmeister, 1838)(13, 47)
Colpocephalum turbinatum Denny, 1842
Columbicola columbae (Linnaeus, 1758)
Hohorstiella lata (Piaget, 1880)
Menacanthus stramineus (Nitzsch, 1818)×
Menopon gallinae (Linnaeus, 1758)×
Goniodes sp.
Streptopelia senegalensis (Linnaeus, 1766)Laughing doveColumbicola columbae (Linnaeus, 1758)(48)
Streptopelia turtur (Linnaeus, 1758)European turtle doveColpocephalum pectinatum (Osborn, 1902)(38)
Strigiphilus sp.×
CORACIIFORMES
Alcedinidae
Alcedo atthis (Linnaeus, 1758)KingfisherAlcedoecus annulatus (Ansari, 1955)(38)
Meropidae
Merops apiaster (Linnaeus, 1758)Bee-eaterMeromenopon meropis Clay & Meinertzhagen, 1941(49)
Meropoecus meropis (Denny, 1842)
Meropsilla apiastri (Denny, 1842). Reported as Brueelia apiastri
Merops persicus (Pallas, 1773)Blue-cheeked bee-eaterMeromenopon meropis Clay & Meinertzhagen, 1941(49)
Meropoecus meropis (Denny, 1842)
Meropsiella erythropteri (Piaget, 1885). Reported as Brueelia erythropteri
CUCULIFORMES
Cuculidae
Cuculus canorus (Linnaeus, 1758)CuckooCuculoecus latifrons (Denny, 1842). Reported as Philopterus latifron(11)
FALCONIFORMES
Falconidae
Falco cherrug (Gray, JE, 1834)Saker falconColpocephalum sp.(38)
Falco tinnunculus (Linnaeus, 1758)KestrelLaemobothrion maximum (Scopoli, 1763)×This study (38)
GALLIFORMES
Phasianidae
Coturnix coturnix (Linnaeus, 1758)QuailAmyrsidea fulvomaculata (Denny, 1842)(38)
Gallus gallus domesticus (Linnaeus, 1758)ChickenCuclotogaster heterographus (Nitzsch, 1866). Also reported as Lipeurus heterographus(18, 41, 46, 50–59)
Goniodes dissimilis Denny, 1842
Goniocotes gallinae (de Geer, 1778)
Goniodes gigas (Taschenberg, 1879). Also reported as Goniocotes gigas
Lipeurus caponis (Linnaeus, 1758)
Menacanthus pallidulus (Neumann, 1912). Also reported as Menopon pallidulum
Menacanthus stramineus (Nitzsch, 1818). Also reported as Menopon stramineum
Menopon gallinae (Linnaeus, 1758)
Goniodes sp.
Lipeurus sp.
Menopon sp.
Meleagris gallopavo (Linnaeus, 1758)TurkeyChelopistes meleagridis (Linnaeus, 1758)(41, 46, 60)
Goniocotes gallinae (de Geer, 1778)
Goniodes gigas (Taschenberg, 1879)
Menacanthus stramineus (Nitzsch, 1818)
Menopon gallinae (Linnaeus, 1758)
Phasianus colchicus (Linnaeus, 1758)PheasantAmyrsidea perdicis (Denny, 1842). Reported as Amyrsidea hexapilosus(38)
Pavo cristatus (Linnaeus, 1758)PeafowlGoniodes pavonis (Linnaeus, 1758)(61)
Perdix perdix (Linnaeus, 1758)Grey partridgeLipeurus sp.(62)
Menacanthus sp.
Menopon sp.
GRUIFORMES
Rallidae
Rallus aquaticus (Linnaeus, 1758)Water railRallicola cuspidatus (Scopoli, 1763)This study
Fulica atra Linnaeus, 1758CootLaemobothrion atrum (Nitzsch, 1818)This study
PASSERIFORMES
Acrocephalidae
Acrocephalus stentoreus (Hemprich & Ehrenberg, 1833)Clamorous reed warblerBrueelia sp.(14)
Alaudidae
Calandrella rufescens (Vieillot, 1819)Lesser short-toed larkMenacanthus sp.(14)
Galerida cristata (Linnaeus, 1758)Crested larkBrueelia sp.(14)
Ricinus sp.
Corvidae
Corvus corax (Linnaeus, 1758)RavenMyrsidea anaspila (Nitzsch, 1866)(11, 15)
Philopterus corvi (Linnaeus, 1758)
Cuclotogaster heterographus (Nitzsch, 1866)×
Corvus corone (Linnaeus, 1758)Carrion crowPhilopterus ocellatus (Scopoli, 1763)(11, 14, 63)
Brueelia sp.
Cuculoecus latifrons (Denny, 1842). Also reported as Philopterus latifron×
Pica pica (Linnaeus, 1758)Black-billed magpiePhilopterus picae (Denny, 1842)(38)
Emberizidae
Emberiza bruniceps (Brandt, 1841)Red-headed buntingSturnidoecus rostratus (Mey, 1982)(14)
Menacanthus sp.
Emberiza calandra (Linnaeus, 1758)Corn buntingSturnidoecus rostratus (Mey, 1982)(14)
Brueelia sp.
Fringillidae
Chloris chloris (Linnaeus, 1758)European greenfinchMyrsidea sp.(14)
Rhodospiza obsolete (Lichtenstein, 1823)Desert finchBrueelia gobiensis Mey, 1982Reported the bird as Carduelis obsoleta (14)
Philopterus sp.
Fringilla coelebs (Linnaeus, 1758)ChaffinchPhilopterus fringillae (Scopoli, 1772)(14)
Brueelia sp.
Muscicapidae
Saxicola torquatus (Linnaeus, 1766)African stonechatBrueelia sp.(14)
Luscinia megarhynchos (Brehm, 1831)NightingaleBrueelia sp.(14)
Oenanthe lugens (Lichtenstein, 1823)Mourning wheatearPhilopterus sp.(14)
Paridae
Parus major (Linnaeus, 1758)Great titPhilopterus pallescens (Denny, 1842)(14)
Passeridae
Gymnoris xanthocollis (Burton, 1838)Yellow-throated sparrowPhilopterus fringillae (Scopoli, 1772)Reported the bird as Petronia xanthocollis (14)
Passer domesticus (Linnaeus, 1758)House sparrowBrueelia cyclothorax (Burmeister, 1838). Reported as Brueelia subtilis (Nitzsch, 1874)(14)
Philopterus fringillae (Scopoli, 1772)
Sturnidoecus refractariolus (Zlotorzycka, 1964)
Passer montanus (Linnaeus, 1758)Eurasian sparrowBrueelia cyclothorax (Burmeister, 1838). Reported as Brueelia subtilis (Nitzsch, 1874)(14)
Philopterus montani (Zlotorzycka, 1964)
Sturnidoecus ruficeps (Nitzsch, 1866)
Campanulotes compar (Burmeister, 1838)×
Petronia petronia (Linnaeus, 1766)Rock petroniaSturnidoecus refractariolus (Zlotorzycka, 1964)(14)
Brueelia sp.
Philopterus sp.
Phylloscopidae
Phylloscopus collybita (Vieillot, 1817)ChiffchaffBrueelia sp.(14)
Menacanthus sp.
Philopterus sp.
Sturnidoecus sp.
Phylloscopus nitidus (Blyth, 1843)Green warblerBrueelia sp.(14)
Menacanthus sp.
Sturnidae
Acridotheres tristis (Linnaeus, 1766)MynaBrueelia chayanh Ansari, 1955(14, 40)
Myrsidea invadens (Kellogg & Chapman, 1902)
Sturnus vulgaris (Linnaeus, 1758)StarlingBrueelia nebulosa (Burmeister, 1838)(14)
Sylviidae
Sylvia communis (Latham, 1787)WhitethroatSturnidoecus sp.(14)
Turdidae
Turdus ruficollis (Pallas, 1776)Black-throated ThrushPhilopterus sp.(38)
Turdus merula (Linnaeus, 1758)BlackbirdRicinus sp.(14)
PELECANIFORMES
Ardeidae
Ardea purpurea (Linnaeus, 1766)Purple heronMenacanthus sp.×(13)
Egretta garzetta (Linnaeus, 1766)Little egret
Ardeicola sp. Probably Ardeicola expallidus Blagoveshtchensky, 1940(13)
Ciconiphilus decimfasciatus (Boisduval & Lacordaire, 1835)
Pelecanidae
Pelecanus onocrotalus (Linnaeus, 1758)Great white pelicanPiagetiella titan (Piaget, 1880)(4), this study
Pelecanus crispus (Bruch, 1832)Dalmatian pelicanColpocephalum eucarenum (Burmeister, 1838)
PHOENICOPTERIFORMES
Phoenicopteridae
Phoenicopterus ruber (Linnaeus, 1758)American flamingoColpocephalum heterosoma, (Clay, 1951)This study
PODICIPEDIFORMES
Podicipedidae
Podiceps cristatus (Linnaeus, 1758)Great crested grebeAquanirmus podicipis (Denny, 1842)(13)
Pseudomenopon dolium (Rudow, 1869)
Scolopacidae
Numenius arquata (Linnaeus, 1758)CurlewCummingsiella ovalis (Scopoli, 1763)(11, 12, 15)
Quadraceps obtusus (Kellogg & Kuwana, 1902)
Saemundssonia scolopacis phaeopodis subsp. humeralis (Denny, 1842)
Scolopax rusticola (Linnaeus, 1758)Eurasian woodcockLipeurus sp.(19)
Philopterus sp.
STRIGIFORMES
Strigidae
Asio otus (Linnaeus, 1758)Long-eared owlStrigiphilus sp.This study
Athene noctua (Scopoli, 1769)Little owlColpocephalum pectinatum (Osborn, 1902)(38)
Philopterus ocellatus (Scopoli, 1763)×
Bubo bubo (Linnaeus, 1758)Eagle owlStrigiphilus strigis (Pontoppidan, 1763)This study
SULIFORMES
Phalacrocoracidae
Phalacrocorax carbo (Linnaeus, 1758)CormorantPectinopygus gyricornis (Denny, 1842)(13)

Louse species reported from Iran according to their avian hosts until December 2023.

Names of orders are capitalized, and names of families are showed in bold. ×The louse species is not normally found on this bird. Its report is probably due to contamination or misidentification.

Review of all relevant publications indicated that in some reports from Iran, researchers identified the lice specimens only to genus level, i.e., Brueelia (nine documents), Philopterus, Menacanthus (six documents), Ricinus, Lipeurus (three documents), Sturnidoecus, Trinoton, Menopon, and Goniodes (two documents), Ardeicola, Colpocephalum, Craspedorrhynchus, Laemobothrion, Strigiphilus, and Myrsidea (one document) (14, 16, 17, 19, 38, 40, 47, 50, 51, 62). The reason could be damage of the specimens, observation of a louse with morphological differences from identification keys or difficulty in identification of the species. It is necessary that researchers will try their best to identify the lice to species level correctly and provide the drawings, measurements, or photos.

Observation of one male poultry head louse specimen, Cuclotogaster heterographus (Nitzsch, 1866) which was collected from the buzzard Buteo buteo (Linnaeus, 1758) in this study was probably because the buzzard preyed with a galliform bird and the louse was mechanically transferred to the predator. In some reports from Iran, lice species that normally infest other bird orders were documented on abnormal bird species. For instance, Trinoton sp. that infest Anseriform birds were collected from raptors Buteo rufinus and Gyps fulvus (16, 17). It can be assumed that lice infestation occurred during feeding the raptors from their preys. In addition, in some reports, Menacanthus stramineus, Menopon gallinae, and Cuclotogaster heterographus that live on Galliformes were collected from mallards and geese (42, 43) as well as pigeons (47). These findings could be due to keeping mixed species together by nomads which is a normal practice in Iran although misidentification cannot be ruled out. Special caution should be taken for interpretation of such findings.

It is known that both amblyceran and ischnoceran lice can act as vectors or intermediate hosts of helminths, bacteria, and viruses, so it was suggested to delouse the wild birds with insecticides (1) however, we disagree with manipulating host–parasite interactions in the wildlife. Additionally, from the conservation point of view some authors expressed their concerns about co-extinction of the lice with their hosts, e.g., Rallicola extinctus (64) and their extinction during the conservation efforts to save the host, e.g., Rallicola pilgrimi (Clay, 1972) and Colpocephalum californici (31, 64, 65). According to International Union for Conservation of Nature (IUCN), there are concerns regarding decreasing population of several predators such as Aquila nipalensis Hodgson, 1833 steppe eagle (endangered) and Aquila heliaca Savigny, and 1809 Asian imperial eagle (vulnerable) (66). Hence, it is suggested that conservationists consider preserving host-specific lice as part of their efforts to save vertebrate hosts (65).

This study provides the first information about lice infestation of wild birds in different regions of Iran and reports Craspedorrhynchus platystomus, Colpocephalum nanum, Colpocephalum gypsi, Colpocephalum eucarenum, Colpocephalum heterosoma, Degeeriella fulva, Degeeriella fusca, Nosopon chanabense, Nosopon lucidum, Falcolipeurus quadripustulatus Falcolipeurus suturalis, Aegypoecus trigonoceps, Trinoton querquedulae, Anaticola crassicornis, Quadraceps obscurus, Rallicola cuspidatus, and Strigiphilus strigis for the first time from the country. Review of the published data on avian lice fauna of Iran shows that the information is available for almost 14% of the bird species. In contrast, researchers from the neighboring country Turkey have identified over 150 lice species from more than half of the bird species inhabiting the country (21). As Iran and Turkey share many bird species, it seems that many louse species remain to be discovered. Molecular phylogenetic analysis of avian lice from Iran will bring clearer understanding of the role of migratory birds in biogeographic distributions.

Statements

Data availability statement

The original contributions presented in the study are included in the article/Supplementary material, further inquiries can be directed to the corresponding author.

Ethics statement

Ethical approval was not required for the study involving animals in accordance with the local legislation and institutional requirements because examined birds Hamedan province were euthanized by a certified veterinarian of the Provincial Department of Environment because of general health failure prior to transfer to the Faculty of Veterinary Medicine, Bu-Ali Sina University. Birds in other provinces were dead animals.

Author contributions

ZB: Methodology, Writing – original draft. AS: Conceptualization, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing. JK: Methodology, Writing – original draft. MB: Methodology, Writing – original draft. EM: Methodology, Writing – original draft. BD: Investigation, Methodology, Supervision, Writing – original draft, Writing – review & editing.

Funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the Bu-Ali Sina University, Hamedan, Iran (Grant Number 1/1/28864).

Acknowledgments

The authors wish to thank Dr. Mehdi Safikhani, Majid Shabanloo, Reza Daneshpajooh, Dr. Alireza Mohammadi, Javad Noori Azhar (Provincial Department of Agriculture, Hamedan, Iran) and Shirdarreh for their kind cooperation in sample collection; Atabak Roohi-Aminjan (Bu-Ali Sina University, Hamedan, Iran) and Abolghasem Khaleghizadeh (Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization, Tehran, Iran) for the confirmation of bird species; Fatemeh Nikbin and the staff of Laboratory of Parasitology, Faculty of Veterinary Science, Bu-Ali Sina University for their assistance. The authors also thank Mohamamd-Parsa Meeaadfar for providing us with the Trinoton querquedulae specimen.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fvets.2023.1324619/full#supplementary-material

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Summary

Keywords

birds of prey, chewing louse species, fauna, host–parasite associations, Iran, Middle-east, new record, Phthiraptera

Citation

Bahiraei Z, Sazmand A, Khedri J, Babaei M, Moeinifard E and Dik B (2024) Chewing lice of wild birds in Iran: new data and a checklist of avian louse species reported in Iran. Front. Vet. Sci. 10:1324619. doi: 10.3389/fvets.2023.1324619

Received

19 October 2023

Accepted

12 December 2023

Published

02 February 2024

Volume

10 - 2023

Edited by

Calin Mircea Gherman, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Romania

Reviewed by

Bersissa Kumsa, Addis Ababa University, Ethiopia; Luis Garcia Prieto, National Autonomous University of Mexico, Mexico

Updates

Copyright

*Correspondence: Alireza Sazmand,

†These authors have contributed equally to this work

‡ORCID: Zahra Bahiraei orcid.org/0000-0002-7023-1029

Alireza Sazmand orcid.org/0000-0002-8450-2993

Mohammad Babaei orcid.org/0000-0002-1517-7162

Bilal Dik orcid.org/0000-0002-7553-5611

Disclaimer

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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