AUTHOR=Aschalew Natnael D. , Zhang Longyu , Wang Ziyuan , Xia Yuanhong , Yin Guopei , Dong Jianan , Zhen Yuguo , Zhang Xuefeng , Wang Tao , Sun Zhe , Qin Guixin TITLE=Effects of yeast culture and oxalic acid supplementation on in vitro nutrient disappearance, rumen fermentation, and bacterial community composition JOURNAL=Frontiers in Veterinary Science VOLUME=Volume 10 - 2023 YEAR=2024 URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1330841 DOI=10.3389/fvets.2023.1330841 ISSN=2297-1769 ABSTRACT=Intensive sheep production system has led to the utilization of expensive feed ingredients in order to enhance productivity. Our previous research demonstrated the efficacy of oxalic acid (OA) as an essential nutrient in yeast culture (YC) for improving rumen fermentation performance. Consequently, we conducted in vitro experiments to examine the effects of YC and OA on rumen fermentation and bacterial composition. Two diets containing different levels of hemicellulose were formulated: diet 1 with 10.3% and diet 2 with 17% hemicellulose. Three levels of YC (0.00, 0.625, and 1.25 g/kg, DM) and three doses of OA (0.0, 0.4, and 0.8 g/kg) were added into each diet with a 3 × 3 factorial design.A comprehensive assessment was conducted on a total of 18 experimental treatments at fermentation periods of 0, 6, 12, 24, and 48 hours. In the first experiment (diet 1), the supplement of YC, OA, and their interaction significantly increased in vitro DM disappearance (IVDMD) and NDF disappearance (IVNDFD) (P < 0.001). In the second experiment (diet 2), the supplementation of OA and the interaction between YC and OA (P < 0.001) increased IVDMD and IVCPD, but had no significant effects on IVNDFD. The interactions of YC and OA significantly increased ammonia nitrogen (P < 0.001). The production of acetic acid, propionic acid, and total volatile fatty acids (TVFA), and pH were significantly higher in treatments supplemented with YC and OA (P < 0.001). YC and OA in both diets significantly altered the rumen bacterial community composition with increased Shannon and Simpson diversity indices (P < 0.001). In both diets, OA supplement significantly increased the relative abundance of the phylum Bacteroidetes and Prevotella genus. The result also showed a positive correlation between the Prevotella and Selenomonas genera with IVDMD, IVNDFD, propionic acid, and TVFA production, suggesting that these dominant bacteria enhanced rumen nutrient disappearance. In summary, the addition of YC and OA resulted in modifications to the bacterial community's composition and diversity, as well as enhancements in nutrient disappearance. These alterations serve as favorable indications of improved rumen fermentation efficiency, which shed a light for the further in vivo studies.