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BRIEF RESEARCH REPORT article

Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1299379

Susceptibility of primary ovine dorsal soft palate and palatine tonsil cells to FMDV infection

Provisionally accepted
Morgan Sarry Morgan Sarry 1,2*Eve Laloy Eve Laloy 1,3Anthony Relmy Anthony Relmy 1Aurore Romey Aurore Romey 1Cindy Bernelin-Cottet Cindy Bernelin-Cottet 1Anne-Laure Salomez Anne-Laure Salomez 1Hélène Huet Hélène Huet 1Sara Hägglund Sara Hägglund 4Jean-François Valarcher Jean-François Valarcher 4Labib Bakkali Kassimi Labib Bakkali Kassimi 1Sandra Blaise-Boisseau Sandra Blaise-Boisseau 1*
  • 1 Laboratoire de Santé Animale de Maisons-Alfort (ANSES), Maisons Alfort, France
  • 2 AgroParisTech Institut des Sciences et Industries du Vivant et de L'environnement, Paris, Ile-de-France, France
  • 3 Vetodiag, Saint-Pierre-en-Auge, France
  • 4 Host Pathogen Interaction Group, Section of Ruminant Medicine, Department of Clinical Science, Swedish University of Agricultural Sciences, Uppsala, Uppsala, Sweden

The final, formatted version of the article will be published soon.

    Foot and mouth disease (FMD) is a highly contagious viral disease affecting cloven-hoofed animals. This disease is one of the most important in animal health due to its significant socio-economic impact, especially in case of an outbreak. One important challenge associated with this disease is the ability of the FMD virus (FMDV) to persist in its hosts through still unresolved underlying mechanisms. The absence of relevant in vitro models is one factor preventing advancement in our understanding of FMDV persistence. While a primary bovine cell model has been established using cells from FMDV primary and persistence site in cattle, it appeared interesting to develop a similar model based on ovine anatomical sites of interest to compare host-pathogen interactions. Thus, epithelial cells derived from the palatine tonsils and the dorsal soft palate were isolated and cultured. Their epithelial nature was confirmed using immunofluorescence. Following monolayer infection with FMDV O/FRA/1/2001 Clone 2.2, the FMDV-sensitivity of these cells was evaluated. Dorsal soft palate (DSP) cells were also expanded in multilayers at the air-liquid interface to mimic a stratified epithelium sensitive to FMDV infection. Our investigation revealed the presence of infectious virus, as well as viral antigens and viral RNA, up to 35 days after infection of the cell multilayers. Further experiment with DSP cells from different individuals needs to be reproduced to confirm the robustness of the new model of persistence in multilayer DSP. The establishment of such primary cells creates new opportunities for FMDV research and analysis in sheep cells.

    Keywords: Foot-and-mouth disease virus (FMDV), Persistence, Cellular model, primary cells, Sheep, Palatine tonsils, dorsal soft palate

    Received: 16 Oct 2023; Accepted: 15 Jul 2024.

    Copyright: © 2024 Sarry, Laloy, Relmy, Romey, Bernelin-Cottet, Salomez, Huet, Hägglund, Valarcher, Bakkali Kassimi and Blaise-Boisseau. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Morgan Sarry, Laboratoire de Santé Animale de Maisons-Alfort (ANSES), Maisons Alfort, France
    Sandra Blaise-Boisseau, Laboratoire de Santé Animale de Maisons-Alfort (ANSES), Maisons Alfort, France

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.