AUTHOR=Han Yu , Lu Kejia , Zhang Riteng , Wei Xi , Guo Hanwei , Tong Lina , Wang Xinglong , Xiao Sa , Liu Haijin , Yang Zengqi 

TITLE=Construction and characterization of a reverse genetics system of bovine parainfluenza virus type 3c as a tool for rapid screening of antivirals in vitro

JOURNAL=Frontiers in Veterinary Science

VOLUME=Volume 11 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1336663

DOI=10.3389/fvets.2024.1336663

ISSN=2297-1769

ABSTRACT=Bovine parainfluenza virus type 3 (BPIV3) is a key pathogen associated with Bovine respiratory disease complex (BRDC). However, its specific pathogenesis mechanisms have not been fully elucidated. Reverse genetics provides a useful method for understanding the pathogenic mechanism of BPIV3. To ensure the rescue platforms function, we first build a minigenome (MG) system of BPIV3 utilizing a 5-plasmid system in this investigation. Then, we obtained a full-length infection clone of BPIV3 from the SX-2021 strain, and different methods identified the rescued virus.Additionally, we recover a recombinant BPIV3 using the reverse genetics system that could express enhanced green fluorescence protein (eGFP). Through the growth curve assays, the replicate capability of rBPIV3-SX-EGFP was like that of the parental virus.Subsequently, the rBPIV3-SX-EGFP was used for determining the antiviral activity of ribavirin, the result showed that ribavirin had an anti-BPIV3 effect in MDBK cells. In conclusion, the successful development of a reverse genetic system for the SX2021 strain establishes a foundation for future studies on BPIV3, including investigations into its pathogenic mechanism, gene function, and antiviral screening.