AUTHOR=Loor-Giler Anthony , Muslin Claire , Santander-Parra Silvana , Coello Dayana , De la Torre David , Abad Hernán , Nuñez Luis 

TITLE=Simultaneous detection of infectious bronchitis virus and avian metapneumovirus genotypes A, B, and C by multiplex RT-qPCR assay in chicken tracheal samples in Ecuador

JOURNAL=Frontiers in Veterinary Science

VOLUME=Volume 11 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1387172

DOI=10.3389/fvets.2024.1387172

ISSN=2297-1769

ABSTRACT=Respiratory diseases in poultry farming cause serious economic losses due to mortality and morbidity, where the infectious bronchitis virus (IBV) is a pathogen that causes serious outbreaks of the disease due to its rapid dissemination, wide range of genotypes and its rapid genetic variability; Likewise, the avian metapneumovirus has 3 genotypes that spread rapidly and cause outbreaks of respiratory disease, and can act in coinfection between the 2 viruses (IBV and MPVA), due to this, the rapid diagnosis of these diseases is vital to be able to take measures. necessary for the containment, elimination of outbreaks and strategies for their prevention. The present study shows an RT-qPCR assay capable of simultaneously detecting and quantifying IBV and 3 AMPV genotypes, showing itself to be an effective, rapid and useful tool for the early diagnosis of these pathogens that cause respiratory disease in poultry, allowing the identification of pathogens and allows decision-making to control diseases. Furthermore, it shows that some of these viral genotypes are circulating in poultry farming in the region where the study was carried out, due to single infection or coinfections between these viruses.