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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Animal Reproduction - Theriogenology
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1393706
This article is part of the Research Topic Exploring 'Omic' Biomarkers in Animal Production and Reproduction View all articles

Age-dependent variations in proteomic characteristics of spermatozoa in Simmental bull

Provisionally accepted
Faisal A. Satrio Faisal A. Satrio 1Ni Wayan K. Karja Ni Wayan K. Karja 2Mohamad A. Setiadi Mohamad A. Setiadi 2Ekayanti M. Kaiin Ekayanti M. Kaiin 3Berlin P. Pardede Berlin P. Pardede 3Bambang Purwantara Bambang Purwantara 2*
  • 1 Veterinary Medicine Study Program, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia
  • 2 Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Jakarta, Indonesia
  • 3 Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Bogor, Jakarta, Indonesia

The final, formatted version of the article will be published soon.

    Increasing the age of bulls results in a decrease in reproductive function, including a reduction in sperm quality, which plays a vital role in determining the fertility of bulls. Through a proteomic approach, this research aims to analyze the influence of age factors on various proteomes contained in bull sperm. Frozen semen samples from Simmental Bulls were categorized into three age groups: two, four, and ≥10 years old. Subsequently, the post-thaw sperm cells obtained were separated based on molecular weight using 1D-SDS-PAGE. Peptides extracted from the bands produced in each age group were subjected to LC-MS/MS analysis. A total of 72 protein types were identified, with 45 being detected in the four-year-old group and 41 expressed in both the two and ≥10-year-old groups. The results provided insights into proteins' role in sperm metabolism across all age groups. Specifically, the twoyear-old group exhibited the expression of proteins associated with acrosome assembly and spermatid development (SPACA1). In contrast, those in the four-year-old group were linked to motility (PEBP4) and sperm decapacitation factor (PEBP1). Proteins expressed in the two and four-year-old groups were discovered to be involved in fertilization processes (TEX101). In contrast, the ≥10-year-old age group was associated with hyperactive movement related to capacitation (Tubulin). In conclusion, age influenced the differences observed in the proteomic profile of post-thaw Simmental bull sperm using the 1D-SDS-PAGE tandem LC-MS/MS approach. Deleted: The frozen semen originated from Simmental bulls of 80 different age groups, namely two (young; n = 3 bulls or nine straws), 81 four (adult; n = 3 bulls or nine straws), and ≥ ten years old (old; n = 3 82 bulls or nine straws).

    Keywords: age, Bull, LC-MS/MS, post-thawing sperm, proteomic 0019953), fertilization (GO:0009566), and single fertilization which serves as

    Received: 29 Feb 2024; Accepted: 28 May 2024.

    Copyright: © 2024 Satrio, Karja, Setiadi, Kaiin, Pardede and Purwantara. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Bambang Purwantara, Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Jakarta, Indonesia

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