AUTHOR=Liu Yuanyuan , Yang Ning , Tan Chen , Zhang Yunhang , Gao Shuai , Cai Yifei , Zhang Yue , Fu Yuguang , Liu Guangliang , Li Yang 

TITLE=Wuzhishan miniature pig-derived intestinal 2D monolayer organoids to investigate the enteric coronavirus infection

JOURNAL=Frontiers in Veterinary Science

VOLUME=Volume 11 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1457719

DOI=10.3389/fvets.2024.1457719

ISSN=2297-1769

ABSTRACT=Intestinal organoids are valuable tools for investigating intestinal physiology and pathology ex vivo. In previous studies, intestinal organoids of commercial pigs have been developed. Here, we established intestinal organoids derived from Wuzhishan miniature pigs (WZS pigs), a unique kind of pig in the Hainan province of China. Three-dimensional (3D) intestinal organoids and organoid monolayers were developed and assessed. Furthermore, the susceptibility of organoid monolayers of WZS pigs to transmissible gastroenteritis virus (TGEV) was demonstrated. An RNA-seq analysis revealed that the TGEV infection stimulated antiviral and inflammatory immune responses in organoid monolayer models. The study implied the transmission risk of swine enteric coronavirus on WZS pigs and provided useful tools for further research on WZS pigs as laboratory miniature pig models.