AUTHOR=Kmetiuk Louise Bach , Passerino Ana Silvia Miranda , Bonat Marcelo , Morikawa Vívien Midori , Berbigier Alice Pereira , Roque André Luiz Rodrigues , Figueiredo Fabiano Borges , Biondo Alexander Welker 

TITLE=Molecular detection of Trypanosoma minasense in captive black-faced black spider monkeys Ateles chamek (mammalia; primate)

JOURNAL=Frontiers in Veterinary Science

VOLUME=Volume 11 - 2024

YEAR=2025

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1527193

DOI=10.3389/fvets.2024.1527193

ISSN=2297-1769

ABSTRACT=The present study aimed to assess Trypanosoma spp. infection in 17 captive black-faced black spider monkeys (Ateles chamek), living at the Zoological Garden of Curitiba, Paraná, Brazil. Blood samples (3 mL) were collected by femoral puncture by certified veterinarians. A total of 5 μL of extracted blood DNA was submitted to Nested-PCR 18S rDNA. In overall, 4/17 (23.5%) spider monkeys were positive to Trypanosoma spp. by PCR. Sequencing results for 18S rDNA revealed Trypanosoma minasense infection. This is the first report of T. minasense in black-faced black spider monkey worldwide and the first assessment of Trypanosoma species in NHP of southern Brazil.