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        <title>Frontiers in Veterinary Science | New and Recent Articles</title>
        <link>https://www.frontiersin.org/journals/veterinary-science</link>
        <description>RSS Feed for Frontiers in Veterinary Science | New and Recent Articles</description>
        <language>en-us</language>
        <generator>Frontiers Feed Generator,version:1</generator>
        <pubDate>2026-05-12T15:28:57.331+00:00</pubDate>
        <ttl>60</ttl>
        <item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1805893</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1805893</link>
        <title><![CDATA[Larvicidal activity of antiparasitic plant extracts against ovine gastrointestinal nematodes: an in vitro study]]></title>
        <pubdate>2026-05-12T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Chao Ke</author><author>Tu Niya</author><author>Fatimat Shittu</author><author>Md Imranuzzaman</author><author>Dipsana Kc</author><author>Men Su</author><author>Thomas B. McFadden</author><author>Tumen Wuliji</author>
        <description><![CDATA[Increasing resistance of gastrointestinal nematodes (GINs) to synthetic anthelmintics undermines the sustainability of small ruminant production, underscoring the urgent need for alternative control strategies. This study evaluated the in vitro larvicidal activity of aqueous extracts from 24 plant species, including forages, common weeds, and herb species with reported antiparasitic properties. Third-stage larvae (L3) of sheep GINs (predominantly Haemonchus contortus and Strongyloides papillosus) were exposed to each aqueous extract at 500 or 750 μL/mL, and larval mortality was assessed at 12, 24, and 48-h post-exposure. Results showed a clear dose-dependent and time-dependent increase in larval mortality. At the higher dose, five extracts demonstrated ≥90% mortality of larvae at 48 h, including Ageratum conyzoides, Euphorbia esula, Chenopodium album, Abutilon theophrasti, and Eupatorium altissimum. Notably, Ageratum conyzoides and Abutilon theophrasti caused over 85% larval mortality within the first 12 h of exposure, and the high efficacy of plant Eupatorium altissimum and Abutilon theophrasti is a novel finding in this study. Principal component analysis (PCA) of efficacy data grouped the extracts into distinct high-, moderate, and low-efficacy tiers. High-efficacy species (e.g., A. conyzoides, E. esula, and C. album) clustered separately from moderate-efficacy species (e.g., Solidago rugosa, Ocimum basilicum, Albizia julibrissin) and low-efficacy species (e.g., Cannabis sativa, Lespedeza cuneata, Plantago major). Some efficacious plants also exhibited favorable nutritional profiles, for instance, Dalea purpurea, Cichorium intybus, Lotus corniculatus, and Onobrychis viciifolia combined antiparasitic effects with low fiber content and high in vitro digestibility. These results highlight that several plant species merit further evaluations as sustainable botanical dewormers for GIN control. This broad screening provides a foundation for in vivo trials and supports the future development of integrated parasite management strategies for small ruminant production.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1748135</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1748135</link>
        <title><![CDATA[Companion animal owner “types” identified using a large-scale international assessment of the human-animal bond]]></title>
        <pubdate>2026-05-12T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Danny Maupin</author><author>Christos Dadousis</author><author>Anthony D. Whetton</author><author>Nophar Geifman</author>
        <description><![CDATA[IntroductionResearch into the human-animal bond has identified a complex relationship between pet ownership and health with both positive and negative effects. However, research into this bond has been limited by relatively small, heterogenous datasets. This study aims to define strata of cat and dog owners based on a large, multi-national dataset.MethodsData was provided by the International Survey of Pet Owners and Veterinarians, encompassing information on 19,187 dog and cat owners across 10 countries. Strata of cat and dog owners were identified using a model-based clustering approach, with optimal number of clusters identified based on Bayesian Information Criterion (BIC) noting each cluster should contain at least 10% of overall population. Clusters were compared and contrasted across proportion of question responses (χ2 test) and mean response (Mann–Whitney U, Kruskal-Wallis tests).ResultsTwo disparate clusters were identified in dog owners, while three were identified in cat owners. Clusters were associated with different pet ownership characteristics, with subgroups of pet owners (Cluster 2 in dog owners, Clusters 2 and 3 in cat owners) that reported a strong bond with their pet, higher likelihood to spend more money and utilize veterinary products, and a stronger impact on their health. Gender was found to differ between clusters, with female owners tending to report a stronger bond wither their pet.DiscussionOur data-driven approach identified heterogeneity not only between owners of different pet types, but also within owners of the same pet type. Broadly, there appears to be a subgroup of owners with stronger emotional connections with their pet (report the pet is like a child, a stronger bond) compared to owners with a more pragmatic view of their pet. However, results do suggest a high baseline level of care that all owners provide for their pets.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1834007</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1834007</link>
        <title><![CDATA[Regional variations of lipids in camel milk from Xinjiang, China: a UHPLC–MS/MS-based lipidomics study]]></title>
        <pubdate>2026-05-12T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Lin Zhu</author><author>Di Wen</author><author>Zhiwei Li</author><author>Xiushan Tan</author><author>Yusong Shen</author><author>Penglan Dou</author><author>Qiaoye Yang</author><author>Subinur Kurbanjan</author><author>Yanfen Cheng</author><author>Changjiang Zang</author><author>Fengming Li</author>
        <description><![CDATA[Camel milk is widely recognized in Xinjiang for its rich nutritional profile and potential health-promoting properties. Here, we integrated lipidomics and oxylipidomics to deconstruct lipid heterogeneity in camel milk from grazing Bactrian camels across three ecologically distinct regions of Xinjiang, China (Yumin, TC; Huocheng, LC; Fuhai, AC). Critically, our sampling strategy encompassed three ecologically distinct counties, spanning over 400 km—a spatial scale sufficient to capture Xinjiang’s biogeographic gradients. We identified 1,286 significantly differential lipids and 71 oxidized lipids, particularly in phospholipids, sphingolipids, and glycerolipids. Eight exploratory candidate lipids were identified for geographical discrimination: PC(37:4COOH), PC(18:0/18:3), DG(19:2/18:1) (AC); PC(16:2/18:3), PE(18:0/22:2CHO), PE(20:0/18:3), DG(16:0/20:0) (TC); PS(18:0/22:4) (LC). Significant differences in polyunsaturated fatty acids (arachidonic acid, docosahexaenoic acid, eicosapentaenoic acid, linoleic acid) and their oxidation products were observed, reflecting regional forage and environmental impacts. Overall, these findings provide a preliminary basis for understanding region-specific lipid variation in camel milk and a descriptive framework for future validation studies on geographical traceability.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1711781</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1711781</link>
        <title><![CDATA[Health implications of dog-worn equipment: a review of known and alleged physical risks]]></title>
        <pubdate>2026-05-12T00:00:00Z</pubdate>
        <category>Review</category>
        <author>Marcella Ridgway</author>
        <description><![CDATA[Dogs are routinely managed using collars, harnesses and other equipment following practices that have been adhered to for centuries, yet concerns about potential adverse health effects have rarely been addressed through structured investigation. This review presents the existing literature regarding impacts of commonly-used dog-worn equipment, generally limited to studies of generated pressures and leash forces, effects on gait and intraocular pressure and a few case reports of equipment-related trauma. Additional studies are needed to specifically evaluate health risks and assess the validity of claimed adverse impacts of collars and other equipment. Until such evidence is established, heightened awareness of the associations of equipment with important and potentially vulnerable anatomic structures and functions as well as known risks presented herein can serve as a touchstone for safe, thoughtful practice in use of dog-worn equipment by all those who work with dogs.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1810451</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1810451</link>
        <title><![CDATA[Molecular epidemiology and characterization of goose polyomavirus in China: insights into its impact on hatchability and susceptibility to co-infections]]></title>
        <pubdate>2026-05-12T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Yadong Gao</author><author>Lintao Fang</author><author>Yan Wang</author><author>Qingru Chang</author><author>Lingna Zhang</author><author>Junjie Guo</author><author>Jingyi Wu</author><author>Xinran Yao</author><author>Jiaqi Liu</author><author>Junwei Ge</author>
        <description><![CDATA[IntroductionGoose hemorrhagic polyomavirus (GHPV) is the primary etiological agent of goose hemorrhagic nephritis and enteritis (HGNE), a disease causing substantial economic losses to the global waterfowl industry. Despite documented outbreaks in Europe and Asia, integrated molecular epidemiological evidence linking clinical outbreaks, co-infections, and viral phylogeny remains limited.MethodsWe conducted nationwide epidemiological surveillance across eight provinces in China from March 2023 to April 2025, covering 83 farms and 1,902 samples. An outbreak of reduced hatchability was investigated via PCR screening for nine common waterfowl pathogens and bacteriological culture. Virus isolation was performed using embryos from multiple goose breeds, followed by full-genome sequencing and comparative molecular characterization of the isolates.ResultsGHPV was detected in 49% (41/83) of surveyed farms, with an overall individual prevalence of 4% (84/1902). No co-infections were observed in goslings or embryos, but a high co-infection rate of 75% (27/36) was found in GHPV-positive adult geese, with Goose circovirus (GoCV) as the most frequent co-infecting agent (93%). Experimental inoculation showed that Landes goose embryos and their derived primary cells were highly susceptible to GHPV replication, whereas Sichuan White and Sanhua Hybrid geese were not permissive, indicating breed-dependent host–virus interactions. The Chinese isolates exhibited high genomic conservation, with VP1 gene nucleotide homology ranging from 98.3% to 100% among global strains.DiscussionThe combination of low overall prevalence, high co-infection rate in adults, and marked genomic conservation suggests that GHPV may act as an opportunistic pathogen, whose clinical impact is amplified through synergistic interactions with co-infecting agents, particularly GoCV. These findings highlight the need for integrated surveillance targeting co-circulating pathogens and underscore the importance of breed-specific considerations in hatchery management and disease control strategies.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1775401</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1775401</link>
        <title><![CDATA[Cross-sectional analysis of feline gut microbiota reveals differences across age-defined groups under varying environments]]></title>
        <pubdate>2026-05-12T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Yan Wang</author><author>Zehui Chen</author><author>Cong Hua</author><author>Junfu Mao</author><author>Wenjing Geng</author><author>Xiaobo Feng</author><author>Shaotang Ye</author><author>Shanshan Song</author><author>Huanan Wang</author><author>Xiaodu Wang</author><author>Lin Lin</author>
        <description><![CDATA[The gut microbiome plays a critical role in host health; however, its variation across age groups in domestic cats (Felis catus) remains unclear. This study characterized differences in the feline gut microbiota using 16S rRNA gene sequencing in 83 cats across five age-defined groups under varying environments: Pre-weaning (1.5 months, n = 16), Early kitten (3 months, n = 16), Late kitten (6–10 months, n = 15), Young adult (2 years, n = 20), and Mature adult (7–10 years, n = 16). Significant differences in microbial diversity and composition were observed across groups. Alpha-diversity was lowest in Pre-weaning kittens, peaking in Young adults, and declining in Mature adults. Beta-diversity revealed distinct clustering among groups (PERMANOVA, R2 = 0.33, p = 0.001). Sensitivity analysis excluding the heterogeneous Mature adult group showed consistent patterns and increased the explanatory power of age (R2 = 0.48). At the taxonomic level, Pre-weaning microbiota were enriched in Proteobacteria, particularly Escherichia-Shigella. Following weaning, the relative abundance of Negativibacillus increased, whereas Lactobacillus decreased. Bifidobacterium was more abundant in kitten stages, while Faecalibacterium exhibited higher abundance in Young adults. These patterns were generally consistent in sensitivity analyses. In contrast, the Mature adult group exhibited reduced Prevotella and increased Escherichia-Shigella relative to Young adults. Overall, this cross-sectional study identified compositional differences in the feline gut microbiota across age-defined groups under varying environments. These findings should be interpreted as group-level associations rather than independent age effects. Further controlled and longitudinal studies are needed to disentangle these effects.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1722404</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1722404</link>
        <title><![CDATA[Mitogenomic analysis of Palaeopsylla remota and Frontopsylla elata elata with implications for the phylogeny of Siphonaptera]]></title>
        <pubdate>2026-05-12T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Shaobo Tang</author><author>Jun Wu</author><author>Mingna Duan</author><author>Rui Hou</author><author>Lanling Tian</author><author>Zongti Shao</author><author>Wei Gu</author><author>Xing Yang</author>
        <description><![CDATA[Fleas are among the most prevalent hematophagous ectoparasites found on mammals and have the ability to spread various pathogens. This study presents the first sequencing and analysis of the mitochondrial genomes of Palaeopsylla remota and Frontopsylla elata elata, alongside a comparative examination using mitochondrial genomic data from other flea species. Multiple aspects were investigated, including nucleotide composition, tRNA secondary structures, codon usage bias, nucleotide diversity, selective pressure, phylogenetic relationships, and divergence time estimation. Both species possess a typical mitochondrial genome comprising 37 genes and one control region, showing a pronounced A+T bias. Non-canonical G-U and U-U pairings were identified in some tRNA genes. ENC-plot and PR2-plot analyses indicated that natural selection is the main factor shaping codon usage bias in these two fleas. Within Siphonaptera, nucleotide diversity and selection pressure analyses revealed that the cox1 gene exhibited the lowest values for both Pi and Ka/Ks. Phylogenetic reconstructions based on BI and ML methods using PCG123 and PCG12 datasets consistently supported the monophyly of the superfamily Ceratophylloidea and the family Pulicidae, whereas the families Ceratophyllidae, Leptopsyllidae, and Ctenophthalmidae displayed paraphyletic associations. Divergence time estimates suggest that the most recent common ancestor of extant fleas dates back to the Cretaceous period, with significant radiation events occurring after the K-Pg boundary. This study expands the mitochondrial genomic resources for fleas and offers new insights into their evolutionary history and phylogenetic relationships.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1763965</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1763965</link>
        <title><![CDATA[The boron uptake via LAT1 and survival analysis after boron neutron capture therapy in canine hemangiosarcoma cell lines]]></title>
        <pubdate>2026-05-12T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Ryota Iwasaki</author><author>Sho Uchida</author><author>Ryutaro Yoshikawa</author><author>Sachiko Yoshihashi</author><author>Minoru Suzuki</author><author>Takashi Mori</author>
        <description><![CDATA[IntroductionCanine hemangiosarcoma (HSA) is a highly aggressive malignancy with limited effective therapies. Boron neutron capture therapy (BNCT) has begun to be recognized as a potential treatment in human angiosarcoma, but its applicability to canine HSA has not been explored. This study aimed to evaluate L-type amino acid transporter 1 (LAT1)–mediated uptake of boronophenylalanine (BPA), a 10B-containing compound, and the in vitro therapeutic potential of BNCT in canine HSA cells.MethodsThree canine HSA cell lines (JuB2, Ud6, Re21) were assessed for BPA uptake using fluorescence measurements, fluorescence imaging, and inductively coupled plasma–atomic emission spectrometry. LAT1 involvement was examined using the LAT1 inhibitor BCH. Clonogenic assays following X-ray or neutron irradiation, with or without BPA pretreatment, were performed to calculate survival fractions, dose–response curves, D10, relative biological effectiveness (RBE), and compound biological effectiveness (CBE).ResultsAll cell lines demonstrated BPA uptake, which was significantly reduced by BCH, confirming LAT1-mediated transport. Time-course analysis revealed cell line–dependent differences in intracellular 10B accumulation. Neutron irradiation yielded greater cytotoxicity than X-rays, and BPA further enhanced this effect, consistent with the 10B(n, α)7Li reaction. CBE values were 2.60 (JuB2), 2.31 (Ud6), and 1.25 (Re21), aligning with differences in BPA uptake.ConclusionThese findings demonstrate functional LAT1-dependent BPA uptake and BNCT-induced cytotoxicity in canine HSA cells. The results provide foundational radiobiological evidence supporting the feasibility of BNCT in canine HSA and justify further in vivo investigations.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1832183</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1832183</link>
        <title><![CDATA[Genotoxicity and gene expression modulation associated with the use of DDVP antiparasitic drugs in aquaculture on Danio rerio]]></title>
        <pubdate>2026-05-12T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Ad Viralkumar</author><author>Lukram Sushil Singh</author><author>Gowhar Iqbal</author><author>Vivek Shrivastava</author><author>Ridhdhisa R. Barad</author><author>Krinal Mori</author><author>Sujit Kumar</author>
        <description><![CDATA[IntroductionParasites are a significant threat to aquaculture, and the application of various antiparasitic chemicals by aquafarmers is a widely practiced strategy to control them. Few antiparasitic drugs are toxic to hosts at higher concentrations and may be genotoxic to them. Genotoxicity tests have been successfully used as indicators of the toxicity of several drugs and environmental pollutants, including heavy metals. The current study was aimed to assess the toxicity of antiparasitic chemicals or drugs at various levels, with special emphasis on genotoxicity in a Zebrafish model.MethodsAdult zebrafish were acclimatized under laboratory conditions and exposed to graded concentrations of dichlorvos to determine acute toxicity (LC25 and LC50) through a 96 h semi-static bioassay. Based on these values, fish were further subjected to sublethal and lethal concentrations for in vivo assessment at 24, 48, 72, and 96 h intervals. Genotoxicity was evaluated using micronucleus and alkaline comet assays on blood and gill tissues. Gene expression analysis of p53, Cyp1a, and Bcl-2 was performed in gill, liver, and kidney tissues using qRT-PCR after RNA isolation and cDNA synthesis. Data were statistically analyzed by one-way ANOVA followed by Tukey's post-hoc test at p < 0.05.ResultsThis study investigated the genotoxic potential of dichlorvos in zebrafish using micronucleus and comet assays, along with gene expression analysis of key biomarkers (p53, Cyp1a, and Bcl-2). The 96-hour LC50 and LC25 values of DDVP were determined as 10.6 ppm and 6.78 ppm, respectively. Based on sublethal toxicity, zebrafish (Danio rerio) were exposed to four concentrations (2.5, 5, 7.5, and 10 mg/L) for in vivo analysis. A significant, concentration- and time-dependent increase in micronucleus frequency was observed, with a 5.5-fold rise between the lowest and highest concentrations after 24 h (h). DNA damage, assessed through comet assay, was minimal at 24 h but increased non-linearly, reaching maximum levels at 96 h, particularly in the highest exposure group. Gene expression analysis revealed marked upregulation of p53, Cyp1a, and Bcl-2 in gill tissues, especially at higher concentrations. Bcl-2 showed the highest induction (19.1-fold), followed by p53 and Cyp1a, indicating activation of cellular stress, detoxification, and apoptosis-related pathways in response to DNA damage.ConclusionOverall, dichlorvos exposure induced significant genotoxic effects in zebrafish in a dose- and duration dependent manner. The findings suggest that concentrations below LC25 may be relatively safer for therapeutic use. Further studies are required to check the efficacy of DDVP against different parasites to formulate more specific treatment strategies for sustainable aquaculture practices.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1831875</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1831875</link>
        <title><![CDATA[Genomic characterization of mcr-1.1-positive Salmonella Kentucky ST198 from a captive Asian houbara bustard reveals links to broiler poultry in the United Arab Emirates]]></title>
        <pubdate>2026-05-12T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Mar Carrasco Muñoz</author><author>Ana Pérez de Vargas</author><author>Mezna Alhebshi</author><author>Xander Velkeneers</author><author>Loïc Lesobre</author><author>Glindya Bhagya Lakshmi</author><author>Fatma A. Mohamed</author><author>Mohammed Elbediwi</author><author>Ihab Habib</author>
        <description><![CDATA[The Asian houbara bustard (Chlamydotis macqueenii) is a vulnerable species supported by conservation breeding programs internationally, as well as in the United Arab Emirates (UAE). Infectious diseases caused by antimicrobial-resistant pathogens represent an emerging threat to captive populations. This study aimed to characterize the genomic features, antimicrobial resistance (AMR) determinants, and phylogenomic relatedness of an mcr-1.1-positive multidrug-resistant Salmonella Kentucky ST198 isolate recovered from a captive Asian houbara bustard in the United Arab Emirates. Post-mortem bacteriological culture of yolk sac samples yielded S. Kentucky isolate FAZ18016, which underwent antimicrobial susceptibility testing and long-read whole-genome sequencing. The isolate exhibited phenotypic multidrug resistance to 17 antimicrobials, supported by 21 resistance genomic determinants including blaTEM − 1, mph(A), tet(A), floR, sul1/sul3, and mcr-1.1. The colistin minimum inhibitory concentration was 8 mg/L. The mcr-1.1 gene was located on an IncHI2 plasmid co-harboring tet(A), terC, and multiple insertion sequences, sharing >99% nucleotide identity with plasmids described in Klebsiella pneumoniae and Escherichia coli. A total of 156 virulence-associated genes were identified, including complete Salmonella Pathogenicity Island 1 and 2, type III secretion systems and type VI secretion system components. Core-genome single-nucleotide polymorphism analysis assigned FAZ18016 to sequence type (ST) 198 and revealed 31–166 Single nucleotide polymorphism (SNP) differences from previously characterized UAE broiler isolates, with the closest related strains differing by 31–33 SNPs, consistent with relatively recent common ancestry. These findings indicate potential spillover of a high-risk, mcr-1.1–carrying S. Kentucky ST198 clone from poultry into captive wildlife and highlight the need for integrated genomic AMR surveillance and strengthened biosecurity at the wildlife–poultry interface within a One Health framework.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1809078</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1809078</link>
        <title><![CDATA[Effect of dietary inclusion of hazelnut skin or hazelnut skin green extract on growth performance, carcass traits, and meat quality in heavy pigs]]></title>
        <pubdate>2026-05-12T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Katia D’Ambra</author><author>Roberta Trovato</author><author>Alice Cattivelli</author><author>Giovanna Minelli</author><author>Martino Musati</author><author>Claudio Forte</author><author>Silvia Tabasso</author><author>Davide Tagliazucchi</author><author>Domenico Pietro Lo Fiego</author>
        <description><![CDATA[IntroductionIntensive livestock farming has sparked public debate, highlighting the need for sustainable livestock production. One effective strategy to improve both environmental sustainability and meat quality is the inclusion of agro-industrial byproducts in pig diets. This study aimed to investigate the effects of dietary inclusion of hazelnut skin (HS) or its green polyphenolic extract (HSE) on growth performance, carcass traits, and meat quality in heavy pigs.MethodsSeventy-two pigs were allotted to three dietary treatments (n = 24 each): a control group (C) fed a standard diet, and two experimental groups receiving the same diet, including either 0.6% HS replacing 0.6% of wheat bran (HSD) or adding 0.1% HSE (HSED). After growth performance assessment, 36 subjects, balanced by gender and treatment, were slaughtered for carcass and meat quality evaluations.ResultsNo differences were observed in growth performance or carcass yield, indicating all diets met nutritional needs. However, HSE supplementation increased thigh yield (p < 0.01). Overall, meat quality characteristics did not differ among treatments; however, HS inclusion showed positive trends in oxidative stability during cooking, n-3 polyunsaturated fatty acid content, and the n-6/n-3 ratio. HSE supplementation decreased cooking loss, giving it better water-holding capacity. The treated groups showed a reduction in cholesterol content in the meat of 7.56 and 12.10% in the HSD and HSED groups, respectively.ConclusionOverall, the modest yet positive effects, combined with the absence of negative influences on growth performance, carcass traits, and meat quality, indicate that HS and HSE supplementation could offer commercial and environmental benefits in sustainable livestock systems.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1772142</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1772142</link>
        <title><![CDATA[Antimicrobial usage in broiler poultry farms in Zambia]]></title>
        <pubdate>2026-05-12T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Isaac Silwamba</author><author>John Bwalya Muma</author><author>Geoffrey Mainda</author><author>NiwaelJesse MtuiMalamsha</author><author>Chitwambi Makungu</author><author>Kayula Mwila</author><author>Tabitha Kimani</author><author>Mark Caudell</author><author>Mark Obonyo</author><author>Charles Bebay</author><author>Suze Percy Filippini</author><author>Maria Hartmann</author><author>Sandra Brogden</author><author>Alina Kirse</author><author>Lothar Kreienbrock</author>
        <description><![CDATA[Although Zambia and many other countries globally have very high rates of antimicrobial resistance, there is a surprisingly limited amount of quantitative data on antimicrobial use (AMU) to guide important control actions. Here, we used the VetCAb-ID [Veterinary Consumption of Antibiotics–International Documentation; ©Tierärztliche Hochschule (TiHo) Hannover, Germany] system to conduct a prospective longitudinal analysis of AMU in broiler poultry farms in the Lusaka district of Zambia to quantify the use by the treatment frequency (TF) metric. The overall TF was 7, and the median treatment frequency per day (TFpd) was 0.14. Of all flocks, 80% of them received treatment. Metaphylactic treatment was applied to all flocks at all farms. Overall, a total of nine antimicrobial classes were used, namely tetracyclines, aminoglycosides, aminopenicillins, diaminopyrimidines, fluoroquinolones, macrolides, phosphonic acid derivatives, quinolones, and sulphonamides. Tetracyclines were the antimicrobials most commonly used (62.7%), followed by sulphonamides (15.7%). Prophylactic use accounted for the majority (54.9%) of the total AMU in this study. Therapeutic uses of antimicrobials were primarily for digestive (21.14%) and respiratory (23.43%) problems of the chickens. Of all the treatments recorded, a greater proportion (87.8%) were underdosed, and 7.3% were overdosed. This study demonstrates the feasibility of farm-level monitoring of AMU data from Zambian poultry farms and provides the first quantitative trends of AMU in poultry farms in Zambia. Our findings of the widespread use of antimicrobials for prophylaxis, incorrect dosing patterns, and the relative usage of the highest-priority critically important antimicrobials in Zambian broiler poultry farms are suggestive of misuse. There is a need for continued education about the issue of antimicrobial resistance, application of antimicrobial stewardship, and the establishment of monitoring and benchmarking systems.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1807298</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1807298</link>
        <title><![CDATA[Ultrasonographic estimation of central venous pressure using the caudal vena cava to aorta ratio in dogs: a proof-of-concept study]]></title>
        <pubdate>2026-05-11T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Maxime Cambournac</author><author>Isabelle Goy-Thollot</author><author>Céline Pouzot-Nevoret</author>
        <description><![CDATA[ContextCentral venous pressure (CVP) might be used to guide fluid therapy in critically ill patients, but its invasive nature and associated risks have prompted growing interest in non-invasive alternatives such as ultrasonographic vascular indices.ObjectivesTo evaluate the correlation between the caudal vena cava (CVC) to aorta (Ao) (CVC:Ao) ratio and central venous pressure (CVP) and explore the use of this correlation for discriminating CVP values.Materials and methodsIntermittent CVP measurements were obtained using a water-filled fluid column in dogs admitted for renal replacement therapy. Low (LCVP), normal (NCVP) and high CVP (HCVP) were defined as values of <0, between 0 and 5, and >5 cmH2O (centimeters of water pressure), respectively. The sonographic assessment of volaemia (SAV) protocol was used to obtain the CVC:Ao ratio in the end-expiratory phase.ResultsFourteen dogs and 28 CVC:Ao ratio-CVP paired measurements were included. The CVP (in cmH2O) was estimated from the CVC:Ao ratio using the generated equation CVP = 20.9*(CVC:Ao)–19.44 (R = 0.69, p < 0.0001). The median CVC:Ao ratios differed significantly among groups at 0.91, 1.00, and 1.12 for LCVP, NCVP and HCVP, respectively (p = 0.0014). Using a CVC:Ao ratio <0.97, the sensitivity and specificity for detecting LCVP were 87.5 and 72.7%, respectively. Using a CVC:Ao ratio >1.09, the sensitivity and specificity for detecting HCVP were 66.7 and 90.9%, respectively.Clinical significanceThe CVC:Ao ratio may be used as a surrogate non-invasive marker for estimating CVP in spontaneously breathing dogs. A CVP < 0 cmH2O is likely if the CVC:Ao ratio is <0.97, and a CVP > 5 cmH2O is likely if the ratio is >1.09.]]></description>
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        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1811948</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1811948</link>
        <title><![CDATA[3D anatomy of the heart in teaching: differentiating physiological and pathological changes in wild ungulates in central Europe]]></title>
        <pubdate>2026-05-11T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Klára Matějka Košinová</author><author>Alena Říhová</author><author>Rostislav Linda</author><author>Jan Cukor</author>
        <description><![CDATA[IntroductionAccurate knowledge of the heart's anatomy and its physiological variability in wild ungulates is essential for the correct interpretation of necropsy and imaging findings, veterinary diagnostics, and teaching in fields focused on hunting and wildlife management. One of the most frequently misinterpreted structures is the Os cordis, whose occurrence, morphology, and clinical significance in most species of wild ungulates have not yet been sufficiently studied by modern imaging methods. Therefore, the main aim of this study was to systematically describe the occurrence, localization, and morphometric characteristics of the Os cordis of the most common Central European wild ungulates using computed tomography (CT) and to verify the contribution of CT-based 3D models for teaching and distinguishing between physiological and pathological changes in the heart.MethodsA total of 131 hearts of roe deer (Capreolus capreolus), red deer (Cervus elaphus), fallow deer (Dama dama), mouflon (Ovis gmelini musimon), and wild boar (Sus scrofa) were examined using the multidetector CT.ResultsThe presence, location, density, volume, and size of the Os cordis were evaluated. Digital 3D models were created from DICOM data and used for both morphological analysis and teaching purposes. Os cordis was found in all ruminants studied, Os cordis was found in 43 out of 45 roe deer, in 32 out of 36 mouflons, in 17 out of 18 red deer, and in all fallow deer hearts, while it was not found in wild boar. The density and size of the Os cordis increased significantly with age and differed between sexes and species, while volume was primarily influenced by body weight. The integration of 3D models into teaching led to a significant improvement in students' ability to correctly locate and identify the Os cordis.DiscussionThe results demonstrate that CT and 3D modeling are effective tools for cardiac morphology research, veterinary diagnostics, and modern teaching.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1817725</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1817725</link>
        <title><![CDATA[Development of ovine hepatic organoids: a powerful in vitro platform to reduce the number of experimental animals used in metabolism and nutrition assays]]></title>
        <pubdate>2026-05-11T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>María-Cristina González-Montero</author><author>Miguel Criado</author><author>Mercedes Alonso</author><author>Nuria Santos</author><author>Daniel Gutiérrez-Expósito</author><author>F. Javier Giráldez</author><author>Rafael Balaña-Fouce</author><author>Sonia Andrés</author><author>Carlos García-Estrada</author>
        <description><![CDATA[BackgroundThe liver coordinates metabolic processes that determine growth, health, and production efficiency in livestock species. In ruminants, hepatic metabolism is adapted to support glucose homeostasis, lipid partitioning, and nitrogen utilization during ruminal fermentation. Therefore, predictive experimental models are essential for studying nutrient-mediated regulation of liver function. However, conventional two-dimensional hepatocyte cultures fail to reproduce the structural organization and functional complexity of native liver tissue, limiting their translational value.MethodsHepatic organoids were developed from the progenitor cells of the liver of a male Assaf lamb, and were characterized by histological and immunofluorescence analyses, followed by transcriptomic profiling using RNA-seq and functional enrichment analysis. The metabolic response was assessed by adding DL-methionine and/or betaine and analyzing, by RT-qPCR, the expression of genes encoding key metabolic enzymes (e.g., CPT1A and PDH1) and ribosomal proteins representative of cellular proliferation (e.g., RPL22L1).ResultsHepatic organoids formed spherical epithelial structures with defined apical–basal polarity, intact tight junctions, albumin expression, and intracellular glycogen accumulation. Comparative transcriptomic analysis between organoids and native liver tissue supported the conservation of core cellular programs, although differential expression and functional enrichment analyses demonstrated that hepatic organoids exhibit a transcriptional profile biased toward cell proliferation, protein synthesis, and structural remodeling, while genes associated with mature liver metabolic functions, immune responses, and systemic homeostasis were relatively downregulated. Combined treatment with DL-methionine and betaine significantly increased the expression of CPT1A (associated with mitochondrial beta-oxidation) and RPL22L1 genes, indicating a synergistic metabolic response of both compounds.DiscussionOvine hepatic organoids reproduced structural and cellular features of liver tissue, supporting their relevance as an in vitro metabolic model for ruminants. Transcriptomic comparisons revealed incomplete functional maturation relative to adult liver, which is consistent with a progenitor-like hepatic state commonly observed in organoid systems. Nevertheless, the preserved metabolic response of organoids highlights their value for studying nutrient–gene interactions and evaluating dietary interventions prior to in vivo experimentation. This work represents the first report of sheep hepatic organoids and establishes a foundation for their use as predictive in vitro platforms to study hepatic metabolism, nutrient–gene interactions, and feed additive strategies in ruminant livestock.]]></description>
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        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1848905</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1848905</link>
        <title><![CDATA[Correction: One-step and two-step qPCR assays for CAPRV2023: development and application in full-cycle epidemiological surveillance of golden pompano]]></title>
        <pubdate>2026-05-11T00:00:00Z</pubdate>
        <category>Correction</category>
        <author>Heng Sun</author><author>Bissih Fred</author><author>Haoyu Wang</author><author>Jie Huang</author><author>Zihao Wu</author><author>Dandan Wu</author><author>Yishan Lu</author><author>Jichang Jian</author><author>Yucong Huang</author>
        <description></description>
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        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1785223</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1785223</link>
        <title><![CDATA[Vibrio vulnificus outer membrane vesicles induce mitochondrial dysfunction in macrophages via the TNF signaling pathway]]></title>
        <pubdate>2026-05-11T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Jun Li</author><author>Luying Wang</author><author>Yafang Zhou</author><author>Lijie Fan</author><author>Yongliang Lou</author><author>Xingxing Xiao</author><author>Shuai Gao</author>
        <description><![CDATA[Vibrio vulnificus (V. vulnificus) is a highly virulent zoonotic pathogen causing severe septicemia and tissue necrosis in humans, characterized by a cytokine storm. While bacterial outer membrane vesicles (OMVs) are known to mediate host-pathogen interactions, their specific contribution to V. vulnificus-induced immunopathology remains obscure. In this study, we isolated and characterized V. vulnificus-derived OMVs and investigated their impact on murine macrophages (J774A.1). We demonstrate that these OMVs are actively internalized by macrophages in a time-dependent manner, triggering a robust pro-inflammatory response. Transcriptomic analysis and subsequent validation revealed that internalized OMVs activated the TNF signaling pathway, leading to the phosphorylation of NF-κB and MAPK (p38/JNK) cascades. Crucially, OMV exposure induced severe mitochondrial dysfunction, evidenced by ultrastructural disruption and a significant reduction in mitochondrial membrane potential. Notably, pharmacological blockade of the TNF receptor with the antagonist R-7050 effectively attenuated this mitochondrial damage. These findings establish a novel pathogenic mechanism wherein V. vulnificus OMVs exploit the host TNF signaling axis to drive mitochondrial injury, providing new insights into the cellular mechanisms of V. vulnificus lethality and suggesting the TNF-mitochondria axis as a potential therapeutic target.]]></description>
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        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1820849</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1820849</link>
        <title><![CDATA[Case Report: Osteosarcoma following traumatic injury in a juvenile Argentine black and white tegu (Salvator merianae)]]></title>
        <pubdate>2026-05-11T00:00:00Z</pubdate>
        <category>Case Report</category>
        <author>Sungryong Kim</author><author>Yerim Kim</author><author>Younghoon Jo</author><author>Ki-Jeong Na</author><author>Dongwoo Chang</author><author>Namsoon Lee</author><author>Dong-Hyuk Jeong</author>
        <description><![CDATA[Osteosarcoma (OSA) is a malignant neoplasm of mesenchymal origin characterized by osteoid or immature bone production and is rarely reported in reptiles. A 3-month-old Argentine black and white tegu presented with a subcutaneous mass on the left flank, approximately 2 months after a dog bite. Physical and radiographic examination revealed no initial internal abnormalities, but follow-up examination revealed a rapidly enlarging soft tissue mass. Ultrasonography confirmed a well-encapsulated mass, while contrast-enhanced computed tomography demonstrated mineralization, rib osteolysis, and multiple bilateral nodules, indicating a malignant process. Histopathological examination of the excised mass demonstrated features consistent with giant cell-rich osteosarcoma, including irregular bony trabeculae, numerous multinucleated giant cells, and peripheral osteoid matrix. This case highlights the diagnostic challenges of OSA in reptiles, the value of integrating diagnostic imaging and histopathology, and the need for species-specific imaging protocols in ectothermic species, thereby expanding the literature on reptilian neoplasia.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1821436</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1821436</link>
        <title><![CDATA[Canine leishmaniasis seroprevalence in asymptomatic dogs: results from an epidemiological study in endemic Mediterranean countries]]></title>
        <pubdate>2026-05-11T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Manuela Gizzarelli</author><author>Gaetano Oliva</author><author>Ines Balestrino</author><author>Alexandra Beck</author><author>Thomas Blondel</author><author>Alessia Crippa</author><author>Cécile Collignon</author><author>Valentina Foglia Manzillo</author>
        <description><![CDATA[IntroductionCanine leishmaniosis (CanL), caused by Leishmania infantum, is a major zoonotic disease in Mediterranean countries, where dogs act as the primary reservoir for transmission by phlebotomine sand flies. A significant proportion of infected dogs remain in an asymptomatic subclinical (AS) state and may contribute to maintaining the parasite’s transmission cycle. Identifying these AS dogs is essential for disease surveillance and for implementing effective preventive strategies.MethodsFrom January to April 2024, the non-transmission period for sand flies, 548 clinically healthy dogs were enrolled in France, Greece, Italy, and Spain during routine veterinary visits. Dogs underwent clinical examination and rapid immunochromatographic testing for anti-Leishmania antibodies. Demographic and clinical variables were recorded, and statistical analyses were performed to explore associations between test positivity and factors including age, sex, body weight, sample matrix, and country of residenceResultsOverall seroprevalence was 7.3%, with marked variation among countries: France (2.9%), Spain (3.2%), Italy (8.8%), and Greece (19%). Univariable analysis showed no significant association between rapid test positivity and age, sex, or body weight. In contrast, country and sample material (plasma vs serum) were significantly associated with test results. In the multivariable model, only the country remained a significant predictor. A relevant proportion of seropositive asymptomatic dogs was detected among owned healthy dogs in endemic European regions, highlighting their potential epidemiological role in sustaining L. infantum transmission.DiscussionThese findings underscore the crucial role of veterinarians in promoting regular screening of clinically healthy dogs, increasing owner awareness of CanL, and encouraging the use of effective repellent-based parasiticides to reduce the risk of parasite transmission.]]></description>
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        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fvets.2026.1784276</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fvets.2026.1784276</link>
        <title><![CDATA[Personal biosecurity among livestock producers and veterinarians in Armenia and the Republic of Moldova]]></title>
        <pubdate>2026-05-08T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Mafalda Pedro Mil-Homens</author><author>Daniel Beltran-Alcrudo</author><author>Alberto Allepuz</author>
        <description><![CDATA[Livestock producers and veterinarians are at high risk of zoonotic disease exposure, yet protective practices are often inconsistent. Cross-sectional surveys were conducted in Armenia (387 livestock producers and 113 veterinarians) and the Republic of Moldova (373 livestock producers and 100 veterinarians). To analyze how education, knowledge, and risk perceptions influence personal biosecurity practices, specifically the use of personal protective equipment (PPE), descriptive statistics and multiple correspondence analysis (MCA) were employed. The findings revealed gaps in awareness and the implementation of preventive measures, including not using PPE to handle abortion materials or carcass disposal. In addition, risky behaviors such as feeding viscera to pets and selling unpasteurized milk were common. Although 40% of livestock producers reported having no formal training on zoonotic diseases, more than 80% indicated that they would like to learn more about the subject. Veterinarians' results reflected compliance with hygiene standards and a commitment to using PPE and implementing biosecurity measures. Additionally, greater self-reported knowledge on zoonotic diseases was associated with improved hygiene and more appropriate use of PPE in specific situations. The study underscores the critical role of education and effective communication between livestock producers and veterinarians in promoting safe practices. Addressing these gaps requires context-specific training and awareness, provision of affordable PPE, and institutional strengthening of veterinary services, framed within a One Health approach.]]></description>
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