Research Topic

Alternative Gene Editing Reagent Delivery Methods in Plants

About this Research Topic

The delivery of genome editing reagents in plants is typically achieved by the expression of transgenes from T-DNA after Agrobacterium mediated transformation or by particle bombardment or PEG-mediated transfection of DNA or RNPs. These approaches are reliable and relatively simple to implement in standard plant molecular biology labs. However, all of these approaches are limited to plant genotypes that can go through tissue culture and regeneration. This presents a considerable obstacle for some plant species as well as making it difficult to directly engineer desired traits in elite cultivars. To overcome this genotype dependency on plant transformation and regeneration, many researchers are experimenting with alternative gene editing reagent delivery vehicles such as viruses, pollen, and carbon nanotubes, just to name a few.

The objective of this special topic in Frontiers in Genome Editing- Genome Editing in Plants is to provide a comprehensive overview of the different methods being developed to help inspire scientists to further think outside of the box to overcome this challenge. Therefore we are looking for submissions of original research using non-conventional methods of gene editing reagent delivery. Approaches should not include standard Agrobacterium or gene gun transformation nor PEG-mediated transfection of protoplasts.


Keywords: gene editing, delivery vehicles, viruses, pollen, carbon nanotubes, gene editing reagent delivery


Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.

The delivery of genome editing reagents in plants is typically achieved by the expression of transgenes from T-DNA after Agrobacterium mediated transformation or by particle bombardment or PEG-mediated transfection of DNA or RNPs. These approaches are reliable and relatively simple to implement in standard plant molecular biology labs. However, all of these approaches are limited to plant genotypes that can go through tissue culture and regeneration. This presents a considerable obstacle for some plant species as well as making it difficult to directly engineer desired traits in elite cultivars. To overcome this genotype dependency on plant transformation and regeneration, many researchers are experimenting with alternative gene editing reagent delivery vehicles such as viruses, pollen, and carbon nanotubes, just to name a few.

The objective of this special topic in Frontiers in Genome Editing- Genome Editing in Plants is to provide a comprehensive overview of the different methods being developed to help inspire scientists to further think outside of the box to overcome this challenge. Therefore we are looking for submissions of original research using non-conventional methods of gene editing reagent delivery. Approaches should not include standard Agrobacterium or gene gun transformation nor PEG-mediated transfection of protoplasts.


Keywords: gene editing, delivery vehicles, viruses, pollen, carbon nanotubes, gene editing reagent delivery


Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.

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Submission Deadlines

14 August 2020 Manuscript

Participating Journals

Manuscripts can be submitted to this Research Topic via the following journals:

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Topic Editors

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Submission Deadlines

14 August 2020 Manuscript

Participating Journals

Manuscripts can be submitted to this Research Topic via the following journals:

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