About this Research Topic
Many health-related problems, such as cardiovascular disease (CVD), are associated with the formation of an excessive clot in the blood (thrombus). Approaches in CVD treatment include preventing formation or removing the thrombus using thrombolytic agents. These can be classified as plasminogen activators (tPA: tissue plasminogen activator), fibrinolytic enzymes (which directly degrade fibrinogen or fibrin), and heparin-type (which act as thrombin inhibitors). Recently, microbial fibrinolytic enzymes originating from food have received more attention, leading to escalating efforts in exploring traditional fermented foods as safe natural sources of these agents.
Traditional fermented food is a safe source of microorganism-produced fibrinolytic enzymes. Therefore the screening, isolation and characterization (including identifying mechanisms of action) of unique fibrin degrading enzymes from this natural source is important in the search for safe and efficient thrombolytic agents for health applications. Finding fibrinolytic enzymes from microorganisms with multiple actions, such as in fibrin degradation, plasminogen activators, and thrombin inhibitors, should be encouraged to provide even more efficient thrombolytic agents.
There are now numerous bioinformatics tools available to explore the molecular structure of proteins and the genes encoding enzymes. Bioinformatic tools can be used to analyze interactions between enzymes and other proteins with natural substrates and potential inhibitors. This basic information will be important to prepare stable thrombolytic agents useful for therapeutic application. Exploration of the gene encoding the enzymes and proteins from these microorganisms will allow acceleration of their production and application, as well as providing information on the molecular aspects of the enzyme from the genetic level.
This Research Topic aims to cover the following themes:
• Screening and isolation of unique fibrin and fibrinogen degrading enzymes from fermentation products
• Biochemistry of fibrinolytic enzymes and protein and their mechanism of action
• Purification of enzymes proteins and bioactive molecules with thrombolytic actions
• Cloning and expression of gene encoding fibrinolytic enzymes and proteins
• Bioinformatic study of enzyme and protein with thrombolytic mechanism
• Production and preparation of thrombolytic agents
We especially welcome submissions in the form of original research and reviews.
Presently Dr. Raymond R Tjandrawinata is a full time employee of Dexa Medical Group where he is head of Dexa Laboratory Biomolecular Science. He is also a lecturer in Biomedics in the Faculty of Biotechnology at Atma Jaya Catholic University. All other Topic Editors declare no competing interests.
Research Topic Image - Interaction of Fibrinolytic Enzyme from Indonesian Bacillus subtilis K2 with B domain of Fibrin
Keywords: Fibrin degrading enzymes, Enzyme biotechnology, Bioactive peptide
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