About this Research Topic
In the past two decades, roboticized cell-level micro-manipulation has made great progress, which has achieved remarkable results in reducing the labor intensity of operators and improving the efficiency, such as roboticized injection of blastocyst or monosperm and so on. The 21st century is recognized as the era of brain science. Neurons are the basic unit for the study of brain science. By studying the electrophysiological and morphological characteristics of neurons, the mechanism of the brain can be better explained. Patch clamp technology is the gold standard for studying and recording the electrophysiological activities of neurons. With the rapid development of robotic technology, the robotized micro-manipulation of neurons represented by the patch clamp technology is also undergoing rapid development, such as robotic blind patch clamp technology, robotic visible patch clamp technology of brain slice, robotic two-photon targeting patch clamp technology in vivo and so on.
The research objective of this topic is mainly to solve the technical difficulties in the detection and micro-manipulation of neurons in vivo or in vitro. It mainly focuses on the related technologies of robotic micro-manipulation of neuron, including but not limited to robotic blind patch clamp technology, accurate detection and segmentation of neurons in micro-image, motion control and planning of pipettes in brain tissue, robotic patch clamp technology based on precise sealing, and robotic two-photon targeted patch clamp technology, etc. Robotic technology is used to further study the electrophysiological and morphological characteristics of nerve cells to explain some problems in brain science.
• Develop a system for robotic neuron operation;
• Develop a robotic patch clamp system or a robotic two-photon targeted patch clamp system;
• To study a new problem in the field of neuroscience by using robotic neuron operating system;
• Design a method to detect, segment or classify neural cells in microscopic images accurately;
• Design the motion control and planning algorithm of micromanipulator in brain tissue;
• The research status of roboticized neuron operation and detection is reviewed;
• A database developed for neuron recognition and classification.
Keywords: Roboticization; neuron electrophysiology; neuron detection; patch clamp; micro-manipulation
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