About this Research Topic
Of the detection schemes available for electrochemical nucleic acid-based sensors, electrochemistry of functional nucleic acid recognition has drawn increasing interest due to enabling high sensitivity, high affinity, wide dynamic range, and fast response using equipment of relatively low cost. Electrochemical detection can also be coupled readily with available miniaturization technologies. Single strand DNAs or RNAs bound to their complementary strands with high specificities are useful for nucleic acid detection. One of the iconic DNA segments known as an aptamer can bind to a diverse range of analytes, including metal ions, organic dyes, amino acids, oligosaccharides, peptides, toxins, enzymes, viral proteins, or even cells and bacteria. Under certain conditions, the designated DNA aptamers and DNAzymes are specific to targets being examined. Few articles have been published in the field of electrochemical sensing related to DNA and nucleic acid hybridization, and not many cases have been exploited in real clinical and industrial usage.
The objective of this Research Topic is to become the point of reference for articles that highlight the technical improvements currently being developed and the fabrication of nucleic acid probes for diagnostic applications that could enhance quality of healthcare. We aim to discuss DNA aptamers for targeting diseases, multiplexing detection systems, synthetic DNA mimic immobilization strategies, electroactivity of nucleic acids, the mechanism of electrochemical detections, DNAzyme with peroxidase activity for signal amplification, electrochemical detection based on aptamer structural changes, as well as challenges and suggestions to improve the application of nucleic acid-based sensor at point-of-care setting. Finally, the limitations of current nucleic-based sensors can be discussed along with consideration of future research directions.
We welcome submissions of Original Research, Review, and Perspective articles. Prospective themes can include, but are not limited to:
• Electrochemical nucleic acid-based sensing techniques proposed for the discrimination or detection of metals, toxins, parasites, pathogens, virus, or microbial organisms that are potentially harmful to human health for clinical applications
• Improved detection features or combinatorial selection of electrochemical nucleic acid-based sensing designs
• Incorporating nucleic acid fragments with other nanomaterials, polymers, or peptides for electrochemical biochemical analyte determination
• Multiplex bioassay detections with labeled or label-free target molecules
• Reviews of current nucleic acid-based electrochemical methods or sensing platforms related to point-of-care diagnostics
We would like to acknowledge Dr Surachate Kalasin, who has acted as a coordinator and has contributed to the preparation of the proposal for this Research Topic.
Keywords: Nucleic acid-based, DNA/RNA, aptamers, enzymes, multiplex bioassay, electrochemical sensors, diagnostics
Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.