About this Research Topic
Proteome refers to the entire set of proteins expressed by the whole genome in cell, tissue, or organism at a certain time. It changes dynamically in space and time, and can reflect the function of cells and the state of disease in real time, so it has broad research prospects. The task of proteomics is to study composition, structure, function and interaction of the proteins directing the activities of each living cell. There are tens of thousands of proteins with a wide dynamic range in a complex biological system. Therefore, it requires high-resolution separation techniques and high-sensitivity detection methods for separation and analysis.
After nearly three decades’ development, MS-based proteomics has been widely used as an important research tool in many fields such as finding disease markers, screening drug targets, studying the structure and function of protein complex or protein post-translational modifications, and so on. In routine proteomics research, there are three conventional sample preparation methods, in solution digestion, in-gel digestion and filter aided sample preparation method. However, these methods have serious sample loss, and required large amount of the starting material for deep proteomics analysis, usually at least ten thousands cells.
Simultaneously, one of the major goals of proteomic research is to be able to monitor all proteins in a particular biological system, such as a cell type or cellular subfraction, and biopsy sample. In these cases, the sample amount is very limited, and there is no PCR-like amplification technique for proteins. Therefore, it is necessary to develop more sensitive sample preparation methods. High-resolution separation methods play an extremely important role in the field of proteomics analysis. Capillary reversed-phase liquid chromatography (RPLC) is the dominant peptide separation technique. However, the nonspecific interactions between peptides and RP resins would lead to sample loss during separation.
Alternatively, capillary zone electrophoresis (CZE) has the advantages of high separation column efficiency, low sample volume consumption, and fast separation speed. It has great advantages in high sensitivity proteomics analysis. Combination of capillary RPLC and CZE will provide more comprehensive peptide identifications from mass-limited complex proteome samples. To further improve the sensitivity, it is necessary to develop an integrated platform combing online sample preparation and separation method.
In this Research Topic, we would like to focus on the novel technologies and applications of ultrasensitive proteomics through capillary zone electrophoresis. Potential sub-topics include but are not limited to:
• Innovative sample preparation methodology for mass-limited material
• Novel separation technology for ultrasensitive proteomics analysis
• Discovery and targeted proteomics
• Implementation of novel strategies towards the study of post-translational modifications, protein-protein interaction, drug target screening and disease biomarker discovery
Work around capillary isolectric focusing (cIEF-MS) strategies is also very strongly encouraged.
Topic Editor James (Qiangwei) Xia is the founder and CEO of CMP Scientific that develops, manufactures, and sells capillary electrophoresis and CE−MS ion sources. The other Topic Editors declare no competing interests
We would like to acknowledge Dr Evgenia Shishkova, who has acted as a coordinator and has contributed to the preparation of the proposal for this Research Topic.
Keywords: ultrasensitive, proteomics, sample preparation, capillary zone electrophoresis, mass spectrometry, post-translational modifications, protein-protein interactions, drug targets, disease biomarkers
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