About this Research Topic
Multi-dimensional imaging and high-throughput sequencing technologies (including bulk or single-cell transcriptomics and antigen receptor repertoire profiling) create a wealth of data that enable unprecedented insights into human immune responses. Application of such technologies to human tissue at the site of disease is a powerful approach to further our understanding of the complex and multi-facetted processes that occur in vivo, and which cannot be sufficiently and accurately replicated in vitro or in animal models. Multi-disciplinary teams including clinicians, wet lab scientists, bioinformaticians and computational immunologists are required to successfully acquire and process relevant samples, and to generate, interpret and translate the resulting data.
Over the past decade, much has been learnt about immune system function in health and disease through the application of transcriptomics, proteomics, metabolomics, and immune receptor profiling to whole blood and PBMC from healthy volunteers and patients. However, immune response complexity is heavily influenced by both tissue-specific cellular recruitment and microenvironmental factors, features not captured by studies of blood. The goal of this Research Topic is to showcase how advances in systems immunology can be coupled with human tissue sampling, opening the opportunity to study immunological processes in their natural context. Human tissues may be obtained from patients via traditional surgical routes or from volunteers through innovative human challenge models. They contain tissue-specific cell types, conditioned by the unique features of the local microenvironment. Multi-dimensional profiling of tissue enables researchers to capture immune response networks in great depth and, may allow for integration of spatial and kinetic information, thus helping to establish a comprehensive picture of human in vivo immunology.
In this Research Topic, we welcome the submission of Original Research, Review, Mini-Review, Methods, Technology and Code and Opinion articles that cover, but are not limited to, the following areas:
1. Studies using multi-dimensional analysis of human tissue immune responses to provide insights into physiology or pathophysiology. We encourage contributions from a variety of research fields, including infectious and inflammatory diseases, cancer, vaccination responses, changes throughout life, impact of lifestyle, comparison of tissue sites.
2. Approaches and human experimental models that allow for multi-dimensional analysis of human tissue samples. Examples might include novel controlled human infection models or the development of methods facilitating minimally-invasive autopsies or biopsies.
3. Computational tools that can be applied to large-scale data obtained from human tissue samples.
Keywords: Human tissue, multi-dimensional profiling, in vivo immunology
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