About this Research Topic
Nature has evolved to organize bioactivities from multiple length scales through protein assembly within a complex intracellular environment. Protein assemblies are prevalent not only in prokaryotes but also eukaryotes, including multienzyme complexes, protein clusters, metabolons, signalosomes and phase-defined membrane-less organelles. Assembled proteins allow cells to achieve spatial and temporal control over cellular activities, leading to highly orchestrated metabolisms. Inspired by naturally occurring protein assemblies, there is a growing interest in developing artificial protein assemblies towards applications for synthetic biology, cellular engineering, biomedicine and biotechnology.
Notwithstanding the synthetic protein assemblies has been successful in various applications, it faces two main challenges in its in-depth applications:(1) the limited strategies for designer protein assemblies, and (2) the limited new fields of applications. When assembled proteins were used as a macromolecular drug, it will inevitably affect the circulatory system, immune system, and other body environment. A comprehensive evaluation of the safety of protein self-assembly drugs should be done to provide a theoretical basis for the clinical potential of protein assembly products. It is necessary to discuss process optimization, effectiveness evaluation and novel assembly strategies.
The scope of this Research Topic includes the efficacy and mechanism evaluation of artificial or natural multicomponent protein drugs in critical diseases, as well as the development and optimization of strategies for protein assembly.
We welcome original research, systematic review, review, mini review, and brief research report, covering but not limited to, the following points:
1. Novel immunotherapeutic drugs or targeted therapeutic drugs based on protein complexes constructed in vitro
2. Focus on the preclinical studies of protein complexes on the body's circulatory system and immunity
3. New mechanisms and techniques of protein complex construction
4. Discovery of new components of protein complexes
5. Evaluation of the effectiveness and safety of protein complexes
6. In vitro or in vivo molecular therapeutics and translational medicine for human diseases
Please note that clinical studies and manuscripts describing purely bioinformatic analyses of existing databases without significant experimental validation will not be accepted to this collection, as they are outside the scope of the Preclinical Cell and Gene Therapy section, of which this Research Topic is a part.
Keywords: Protein self-assembly, Functionalization of protein assemblies, Self-assembled, Proteins-based reactors, Membraneless organelles, Tumor therapy, Drug targeting, Drug delivery, Human pluripotent stem cells, Tissue development, Protein assembly, Bio complexes, Gene therapy, Cell therapy
Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.