The advent of next-generation sequencing (NGS) technology, including pyrosequencing (454; Roche) and sequencing by synthesis (Solexa genome analyzer; Illumina), has facilitated the use of metagenomic analyses to characterize viruses in many host plants. In basic virology, diagnostic virology, and disease prevention and control, NGS-based surveys of natural hosts have become important research tools. Ongoing climate changes and the global market are often responsible for the spread and geographical expansion of many phytoviruses, some of which, in new areas, become emerging and very harmful to crops.
HTS is a good alternative to other diagnostic tests, especially in the context of unexpected or unknown viruses that might be potential threats to plant health. Knowledge of the occurrence and degree of infestation of crops, weeds, trees, and ornamental plants allows the introduction of appropriate regulations and thus prevents the occurrence of an epidemic, as well as allowing for the development of new strategies for plant protection.
In this Research Topic, we aim to collect scientific works that serve as a reference for scientists regarding the latest applications of techniques allowing the analysis of plant viromes in phytovirology. Particularly, this method is used to identify emerging or re-emerging viruses that often fail to be recognized by classical diagnostic methods.
Through the use of HTS techniques, this article collection will compile results regarding the discovery of new viruses or different variants of known viruses, with specific attention to the most recent implementations of the major technologies currently being used. In particular, NGS will be emphasized as a tool for resolving phylogenetic relationships in phytovirology and improving the specificity of molecular diagnostics, providing the necessary information for the development of new advanced methods or improving existing ones.
Keywords:
phytovirology, plant protection, NGS technology, solexa genome analyzer, metagenomic analyses, diagnostic virology, ultra-high throughput
Important Note:
All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.
The advent of next-generation sequencing (NGS) technology, including pyrosequencing (454; Roche) and sequencing by synthesis (Solexa genome analyzer; Illumina), has facilitated the use of metagenomic analyses to characterize viruses in many host plants. In basic virology, diagnostic virology, and disease prevention and control, NGS-based surveys of natural hosts have become important research tools. Ongoing climate changes and the global market are often responsible for the spread and geographical expansion of many phytoviruses, some of which, in new areas, become emerging and very harmful to crops.
HTS is a good alternative to other diagnostic tests, especially in the context of unexpected or unknown viruses that might be potential threats to plant health. Knowledge of the occurrence and degree of infestation of crops, weeds, trees, and ornamental plants allows the introduction of appropriate regulations and thus prevents the occurrence of an epidemic, as well as allowing for the development of new strategies for plant protection.
In this Research Topic, we aim to collect scientific works that serve as a reference for scientists regarding the latest applications of techniques allowing the analysis of plant viromes in phytovirology. Particularly, this method is used to identify emerging or re-emerging viruses that often fail to be recognized by classical diagnostic methods.
Through the use of HTS techniques, this article collection will compile results regarding the discovery of new viruses or different variants of known viruses, with specific attention to the most recent implementations of the major technologies currently being used. In particular, NGS will be emphasized as a tool for resolving phylogenetic relationships in phytovirology and improving the specificity of molecular diagnostics, providing the necessary information for the development of new advanced methods or improving existing ones.
Keywords:
phytovirology, plant protection, NGS technology, solexa genome analyzer, metagenomic analyses, diagnostic virology, ultra-high throughput
Important Note:
All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.