Biofilm and quorum sensing are pivotal in enhancing microbial pathogenicity and resilience, posing significant challenges in treating life-threatening human diseases. Biofilms, which are structured bacterial communities encased in an extracellular matrix, provide a protective shield for bacteria against the host immune system and antibiotics, leading to persistent and difficult-to-treat infections. Quorum sensing, a cell-to-cell communication mechanism, regulates biofilm formation by coordinating gene expression based on population density, thereby increasing biofilm production, virulence factors, and antibiotic resistance. These biofilm-forming bacteria are often associated with chronic wounds, urinary tract infections, and lung infections, resulting in increased morbidity, prolonged treatment durations, and elevated healthcare costs. Despite advances in understanding quorum sensing circuits, there remains a critical need for therapeutic strategies to effectively combat biofilm-associated infections. Recent studies have begun to unravel the complex mechanisms of biofilm resilience, yet significant gaps remain in translating these findings into practical disruption strategies.This research topic aims to integrate cutting-edge studies utilizing multi-omics approaches, including genomics, transcriptomics, proteomics, metabolomics, and computational biology, to decode the complex mechanisms underlying biofilm resilience. By understanding these intricate networks, the research seeks to develop novel and effective strategies for biofilm disruption and control. The primary objectives include identifying essential genes and regulatory networks, profiling gene expression, and exploring the roles of non-coding RNAs, proteomics, and metabolomics in biofilm resilience. Additionally, the research will focus on innovative disruption methods and the application of advanced technologies to manage biofilms across diverse environments.To gather further insights into the complex mechanisms of biofilm resilience and control, we welcome articles addressing, but not limited to, the following themes:- Genomic, transcriptomic, proteomic, and metabolomic insights into biofilm resilience, including essential genes, regulatory networks, and metabolic pathways.- Structural biology approaches to study and control biofilm formation, including high-resolution imaging and computational modeling.- Computational and systems biology of biofilms, focusing on environmental effects, ecological interactions, and the development of novel antimicrobial drugs and biofilm-resistant materials.- Synthetic biology and genetic engineering techniques, such as CRISPR and gene knock-out strategies, to enhance biofilm control.
Biofilm and quorum sensing are pivotal in enhancing microbial pathogenicity and resilience, posing significant challenges in treating life-threatening human diseases. Biofilms, which are structured bacterial communities encased in an extracellular matrix, provide a protective shield for bacteria against the host immune system and antibiotics, leading to persistent and difficult-to-treat infections. Quorum sensing, a cell-to-cell communication mechanism, regulates biofilm formation by coordinating gene expression based on population density, thereby increasing biofilm production, virulence factors, and antibiotic resistance. These biofilm-forming bacteria are often associated with chronic wounds, urinary tract infections, and lung infections, resulting in increased morbidity, prolonged treatment durations, and elevated healthcare costs. Despite advances in understanding quorum sensing circuits, there remains a critical need for therapeutic strategies to effectively combat biofilm-associated infections. Recent studies have begun to unravel the complex mechanisms of biofilm resilience, yet significant gaps remain in translating these findings into practical disruption strategies.This research topic aims to integrate cutting-edge studies utilizing multi-omics approaches, including genomics, transcriptomics, proteomics, metabolomics, and computational biology, to decode the complex mechanisms underlying biofilm resilience. By understanding these intricate networks, the research seeks to develop novel and effective strategies for biofilm disruption and control. The primary objectives include identifying essential genes and regulatory networks, profiling gene expression, and exploring the roles of non-coding RNAs, proteomics, and metabolomics in biofilm resilience. Additionally, the research will focus on innovative disruption methods and the application of advanced technologies to manage biofilms across diverse environments.To gather further insights into the complex mechanisms of biofilm resilience and control, we welcome articles addressing, but not limited to, the following themes:- Genomic, transcriptomic, proteomic, and metabolomic insights into biofilm resilience, including essential genes, regulatory networks, and metabolic pathways.- Structural biology approaches to study and control biofilm formation, including high-resolution imaging and computational modeling.- Computational and systems biology of biofilms, focusing on environmental effects, ecological interactions, and the development of novel antimicrobial drugs and biofilm-resistant materials.- Synthetic biology and genetic engineering techniques, such as CRISPR and gene knock-out strategies, to enhance biofilm control.