Impact Factor 4.300
2017 JCR, Clarivate Analytics 2018

The world's most-cited Neurosciences journals

Methods ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Cell. Neurosci. | doi: 10.3389/fncel.2019.00247

Generation of oligodendrocyte progenitor cells from mouse bone marrow cells

 Yuan Zhang1,  Xin-Yu Lu1,  Giacomo Casella2, Jing Tian3, Ze-Qing Ye1, Ting Yang1, Juan-Juan Han1, Ling-Yu Jia3, Abdolmohamad Rostami2 and  Xing Li1*
  • 1Shaanxi Normal University, China
  • 2Neurology, Thomas Jefferson University, United States
  • 3College of Life Sciences, Shaanxi Normal University, China

Oligodendrocyte progenitor cells (OPCs) are a subtype of glial cells responsible for myelin regeneration. Oligodendrocytes (OLGs) originate from OPCs and are the myelinating cells in the central nervous system (CNS). OLGs play an important role in the context of lesions in which myelin loss occurs. Even though many protocols for isolating OPCs have been published, their cellular yield remains a limit for clinical application. The protocol proposed here is novel and has practical value; in fact, OPCs can be generated from a source of autologous cells without gene manipulation. Our method represents a rapid, and high-efficiency differentiation protocol for generating mouse OLGs from bone marrow-derived cells using growth-factor defined media. With this protocol, it is possible to obtain mature OLGs in 7-8 weeks. Within 2-3 weeks from bone marrow isolation, after neurospheres formed, the cells differentiate into Nestin+ Sox2+ neural stem cells (NSCs), around 30 days. OPCs specific markers start to be expressed around day 38, followed by RIP+O4+ around day 42. CNPase+ mature OLGs are finally obtained around 7-8 weeks. Further, bone marrow-derived OPCs exhibited therapeutic effect in shiverer (shi) mice, promoting myelin regeneration and reducing the tremor. Here, we propose a method by which OLGs can be generated starting from bone marrow cells and have similar abilities to subventricular zone (SVZ)-derived cells. This protocol significantly decreases the timing and costs of the OLGs differentiation within 2 months of culture.

Keywords: oligodendrocytes, neurospheres, Autologous cells, oligodendrocyte progenitor cells, Bone Marrow

Received: 14 Dec 2018; Accepted: 17 May 2019.

Edited by:

Paulo Henrique Rosado-de-Castro, D'Or Institute for Research and Education (IDOR), Brazil

Reviewed by:

Enrica Boda, University of Turin, Italy
Jorge Matias-Guiu, Complutense University of Madrid, Spain  

Copyright: © 2019 Zhang, Lu, Casella, Tian, Ye, Yang, Han, Jia, Rostami and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Xing Li, Shaanxi Normal University, Xi'an, China, xingli_xian@126.com