Research Topic

Utilization of Protoplasts to Facilitate Gene-Editing in Plants

About this Research Topic

CRISPR/Cas-mediated editing provides strategies for precise modifications that range from single base pair changes to insertion of DNA fragments into plant genomes. The methods of genetic engineering, including DNA uptake into protoplasts, play a critical role in CRISPR/Cas-mediated gene editing in plants. Protoplast methodologies have experienced a renaissance with the advent of gene editing. Although utilization of protoplasts was once thought to be a methodology of the past, they are now routinely utilized for applications such as testing efficiencies of gRNA designs, DNA-free gene editing, prime editing, and homology-directed repair. The reason for this resurgence of protoplasts is because the delivery of CRISPR/Cas components for several editing approaches and recovery of modified plant lines is not as efficient by other genetic engineering methods such as Agrobacterium or biolistics.

The purpose of this Research Topic is to present how protoplasts are helping to facilitate various CRISPR/Cas-mediated gene-editing approaches in plants. By highlighting the latest research on protoplast technology for various species in addition to their utilization for gene editing, our intent is to provide readers with information to support their efforts with CRISPR/Cas approaches. Protoplasts are playing an important role in testing gRNA designs, DNA-free gene editing, base editing, and prime editing. While isolation of protoplasts can be readily achieved, the more technologically challenging part is determining the optimal conditions for their survival and sustained division for the generation of callus and regeneration of modified plants. Therefore, this Research Topic will include reports for protoplast methodologies along with those exploiting them to achieve gene editing.

This Research Topic welcomes Reviews, Mini-Reviews, Original Research, and Method articles on:

• The history of protoplast technology.
• Protoplast methodologies isolation and recovery of plants.
• Transformation by direct DNA uptake.
• CRISPR/Cas-mediated editing that utilizes protoplasts.
• Protoplast methodology for plant regeneration of various species.
• Transformation by direct DNA uptake.
• Determining gRNA efficiency design by utilizing protoplasts
• Editing approaches that rely on direct DNA uptake into protoplasts for recovery of modified plant lines. Editing approaches can include but are not limited to: DNA-free, homology-directed repair, allele/DNA swapping and insertion, prime editing, base editing.
Van Eck is on the Benson Hill scientific advisory board and a consultant for Ohalo Genetics


Keywords: Cas-gRNA ribonucleoproteins, direct DNA uptake, DNA-free, genome editing, prime editing


Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.

CRISPR/Cas-mediated editing provides strategies for precise modifications that range from single base pair changes to insertion of DNA fragments into plant genomes. The methods of genetic engineering, including DNA uptake into protoplasts, play a critical role in CRISPR/Cas-mediated gene editing in plants. Protoplast methodologies have experienced a renaissance with the advent of gene editing. Although utilization of protoplasts was once thought to be a methodology of the past, they are now routinely utilized for applications such as testing efficiencies of gRNA designs, DNA-free gene editing, prime editing, and homology-directed repair. The reason for this resurgence of protoplasts is because the delivery of CRISPR/Cas components for several editing approaches and recovery of modified plant lines is not as efficient by other genetic engineering methods such as Agrobacterium or biolistics.

The purpose of this Research Topic is to present how protoplasts are helping to facilitate various CRISPR/Cas-mediated gene-editing approaches in plants. By highlighting the latest research on protoplast technology for various species in addition to their utilization for gene editing, our intent is to provide readers with information to support their efforts with CRISPR/Cas approaches. Protoplasts are playing an important role in testing gRNA designs, DNA-free gene editing, base editing, and prime editing. While isolation of protoplasts can be readily achieved, the more technologically challenging part is determining the optimal conditions for their survival and sustained division for the generation of callus and regeneration of modified plants. Therefore, this Research Topic will include reports for protoplast methodologies along with those exploiting them to achieve gene editing.

This Research Topic welcomes Reviews, Mini-Reviews, Original Research, and Method articles on:

• The history of protoplast technology.
• Protoplast methodologies isolation and recovery of plants.
• Transformation by direct DNA uptake.
• CRISPR/Cas-mediated editing that utilizes protoplasts.
• Protoplast methodology for plant regeneration of various species.
• Transformation by direct DNA uptake.
• Determining gRNA efficiency design by utilizing protoplasts
• Editing approaches that rely on direct DNA uptake into protoplasts for recovery of modified plant lines. Editing approaches can include but are not limited to: DNA-free, homology-directed repair, allele/DNA swapping and insertion, prime editing, base editing.
Van Eck is on the Benson Hill scientific advisory board and a consultant for Ohalo Genetics


Keywords: Cas-gRNA ribonucleoproteins, direct DNA uptake, DNA-free, genome editing, prime editing


Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.

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Submission Deadlines

05 January 2021 Abstract
05 May 2021 Manuscript

Participating Journals

Manuscripts can be submitted to this Research Topic via the following journals:

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Topic Editors

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Submission Deadlines

05 January 2021 Abstract
05 May 2021 Manuscript

Participating Journals

Manuscripts can be submitted to this Research Topic via the following journals:

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