About this Research Topic
For the past 20 years, the development of genome editing technologies has been primarily focused on improving genome editing reagents and tailoring them to particular organisms and applications. These include work on easier-to-use, programable nucleases for higher efficiency and better specificity. The development of the genome editing technologies based on the prokaryotic CRISPR/Cas (clustered regularly interspaced short palindromic repeat and associated proteins) systems marked one of the most significant breakthroughs in recent years. Due to its simplicity, robust activity, versatility, and multiplexing capability, CRISPR/Cas has become the reagent of choice for genome editing in most organisms.
Moving forward, more effort will be made to develop tools and technologies for large-scale, high throughput, and more precise genome editing. In recent years, the high throughput CRISPR-based gene knock-out system has been reported for gene discovery and functional analysis in animal species. More precise genome editing tools, such as base-editing and prime-editing, have also been developed primarily for animal species. These technologies will undoubtedly also present great potential to expedite gene discovery and functional genomics in plants. In fact, the base-editing technology has already proven to be effective in a few plant species, and large-scale gene knock-out resources have also been initiated for several plants.
This Research Topic will focus on new genome editing technologies and resources to facilitate gene discovery and functional genomics in plants. We would welcome submissions in the form of reviews and original research on developing novel tools, technologies, and strategies for precision, multiplexing, or high throughput genome editing in plants. The areas of research topics include:
• Tools and resources to enable large-scale and high throughput genome editing in plants;
• Novel technologies to enable precise genome editing in plants;
• New tools and technologies to create and identify large-scale chromosomal and genome modifications other than simple gene knock-outs and knock-ins in plants;
• Plant genetic variation resources, such gene knock-out or cis-regulatory element mutants, created through genome editing.
Keywords: Genome-editing, CRISPR, functional genomics, gene discovery, plant
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