Tumor Microenvironment: The Functions and Underlying Mechanisms of Cellular and Non-cellular Components During Cancer Progression and Metastasis

22.9K
views
51
authors
7
articles
Editors
3
Impact
Loading...
Original Research
14 November 2023
3D tumor spheroids derived from HEY and PEO1 cells show a reduction of intracellular LIP. (A) Western blot of FtH1, CD71, and FPN in HEY and PEO1 cells (3D vs. 2D). γ-TUB was used as loading control. Flow cytometry analysis of LIP (B) and ROS (C) amounts quantified by using CA-AM and CM-H2DCFDA respectively, in HEY and PEO1 cells cultured in both 2D, and 3D condition. (D) PI flow cytometric analysis of HEY and PEO1 cells (3D vs. 2D); % of dead cells (PI positive) are reported in each plot. Representative images of their derived 3D tumor spheroids (Scale bar: 100 μm; Magnification: ×20) and relative histogram of the % of dead cells are reported on the right. qRT-PCR of stemness (E) and EMT (F) markers in HEY and PEO1 cells (3D vs. 2D). Results are presented as mean ± SD from three independent experiments. *p-value <0.05; **p-value <0.01; ns: not significant.
3,741 views
13 citations
Article Cover Image
Review
16 May 2023
Lipid metabolism and tumor immunotherapy
Yue Wang
4 more and 
Huazhong Cai

In recent years, the relationship between lipid metabolism and tumour immunotherapy has been thoroughly investigated. An increasing number of studies have shown that abnormal gene expression and ectopic levels of metabolites related to fatty acid synthesis or fatty acid oxidation affect tumour metastasis, recurrence, and drug resistance. Tumour immunotherapy that aims to promote an antitumour immune response has greatly improved the outcomes for tumour patients. However, lipid metabolism reprogramming in tumour cells or tumour microenvironment-infiltrating immune cells can influence the antitumour response of immune cells and induce tumor cell immune evasion. The recent increase in the prevalence of obesity-related cancers has drawn attention to the fact that obesity increases fatty acid oxidation in cancer cells and suppresses the activation of immune cells, thereby weakening antitumour immunity. This article reviews the changes in lipid metabolism in cells in the tumour microenvironment and describes the relationship between lipid metabolism reprogramming in multiple cell types and tumour immunotherapy.

4,829 views
14 citations