Research Topic

Technical Advances in Cryo-Electron Microscopy

About this Research Topic

Technical advances in cryo-electron microscopy (cryo-EM) are revolutionizing structural biology, as samples that could previously only be studied by X-ray crystallography or nuclear magnetic resonance (NMR) can now be structurally analyzed to comparable resolutions by cryo-EM. Two main EM branches have developed that span a broad range of specimen sizes: single particle cryo-EM and subtomogram average for high-resolution protein structures, and tomography for localization of complexes and organelles in cellular structures.

As the field is developing quickly, the scientific community that benefits from cryo-EM is also expanding. To face the high equipment costs and still satisfy the data requirements of the growing community, new facilities for data collection, which also offer skilled support, are continuously being opened or upgraded. Additionally, software packages for image processing are experiencing fast development with new and faster algorithms being developed for image analysis. EM centers are building up IT infrastructures to deal with the huge influx of microscopy data and to optimize their use.

To date, many reviews have been written about the “resolution-revolution” under which near-atomic structures are obtained on a regular basis by cryo-EM, and the main technological advances that have made this revolution possible. In turn, less information is available about its practical aspects, such as sample and grid optimization, facility organization for efficient data collection and sample storage, setups for storage and data processing, or special image processing tricks required for difficult cases. We therefore welcome our colleagues to submit original research papers, reviews, perspectives, methods, or technology reports with a specific “practical” cut, that will help enrich the existing EM community as well as newcomers.


Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.

Technical advances in cryo-electron microscopy (cryo-EM) are revolutionizing structural biology, as samples that could previously only be studied by X-ray crystallography or nuclear magnetic resonance (NMR) can now be structurally analyzed to comparable resolutions by cryo-EM. Two main EM branches have developed that span a broad range of specimen sizes: single particle cryo-EM and subtomogram average for high-resolution protein structures, and tomography for localization of complexes and organelles in cellular structures.

As the field is developing quickly, the scientific community that benefits from cryo-EM is also expanding. To face the high equipment costs and still satisfy the data requirements of the growing community, new facilities for data collection, which also offer skilled support, are continuously being opened or upgraded. Additionally, software packages for image processing are experiencing fast development with new and faster algorithms being developed for image analysis. EM centers are building up IT infrastructures to deal with the huge influx of microscopy data and to optimize their use.

To date, many reviews have been written about the “resolution-revolution” under which near-atomic structures are obtained on a regular basis by cryo-EM, and the main technological advances that have made this revolution possible. In turn, less information is available about its practical aspects, such as sample and grid optimization, facility organization for efficient data collection and sample storage, setups for storage and data processing, or special image processing tricks required for difficult cases. We therefore welcome our colleagues to submit original research papers, reviews, perspectives, methods, or technology reports with a specific “practical” cut, that will help enrich the existing EM community as well as newcomers.


Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.

About Frontiers Research Topics

With their unique mixes of varied contributions from Original Research to Review Articles, Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author.

Topic Editors

Loading..

Submission Deadlines

04 February 2018 Manuscript

Participating Journals

Manuscripts can be submitted to this Research Topic via the following journals:

Loading..

Topic Editors

Loading..

Submission Deadlines

04 February 2018 Manuscript

Participating Journals

Manuscripts can be submitted to this Research Topic via the following journals:

Loading..
Loading..

total views article views article downloads topic views

}
 
Top countries
Top referring sites
Loading..

Comments

Loading..

Add a comment

Add comment
Back to top