Research Topic

Light Sheet Microscopy: from Technological Developments to Prospective Applications

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About this Research Topic

This Research Topic will constitute the Proceedings of the Light Sheet Workshop which will be held from March 19th to 20th 2018 at the Campus Biotech in Geneva, Switzerland. Registration is open until February 18th 2018 and preliminary program is available.

Light Sheet microscopy is a fluorescence ...

This Research Topic will constitute the Proceedings of the Light Sheet Workshop which will be held from March 19th to 20th 2018 at the Campus Biotech in Geneva, Switzerland. Registration is open until February 18th 2018 and preliminary program is available.

Light Sheet microscopy is a fluorescence imaging technique that allows to visualize whole organs or small organisms while preserving their physical integrity i.e. without the need to slice them prior imaging. Although the principle of operation of this technology was developed more than 100 years ago, it is only in the last fifteen years that the community of biologists has started to use such microscopes in a common way. Since that time, this type of microscopy has become a standalone field of research that has never been as active as it is now. Indeed, a broad international community of researchers, engineers, programmers, physicists and biologists contribute to the rapid evolution of this field. Light Sheet microscopy of clarified tissues is highly challenging in different ways. First, tissue clarification preserving the endogenous fluorescence of genetically encoded proteins or respecting the ability to immunolabel specific cellular populations remains problematic. Second, the imaging technology itself needs to be improved such that it becomes possible to image faster, with better axial and in-plane spatial resolution and with a higher sensitivity in thick tissues. Finally, this imaging modality is known to generate gigantic amounts of data (up to tens of Terabytes per experiment). There is, nowadays, no universal technique that is efficient enough to process, analyze and exploit this huge amount of digital data. It is therefore necessary to develop, together, some standards of imaging technologies and image analysis.

At the Wyss Center for Bio and Neuroengineering in Geneva, Switzerland, we seek to create an event around this imaging technology to be able to federate as many people as possible with different and complementary skills. Our goal is also to promote and strengthen a community that could interact, share and will contribute to tackle these challenges.

Publishing the Proceedings of the Light Sheet Microscopy workshop as a Research Topic will help us to share with the largest Light-Sheet community, the scientific content that will be exchanged during this event. Additionally, we welcome the work from members of the Light-Sheet microscopy community not attending the workshop. Indeed, we want to create a dynamic amongst greatest number of researchers by offering them the opportunity to share their own research and to disseminate it broadly.

All different kinds of papers are welcomed in this Research Topic including Original Research, Reviews, Protocols and methodological articles.


Keywords: Light Sheet Microscopy, Tissue Clarification, COLM, MesoSPIM, DISCO, Big Data management, Stitching, 3D registration


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