Development and Application of Novel Genome Engineering Tools in Microbial Biotechnology

235.9K

views

124

authors

22

articles

Development and Application of Novel Genome Engineering Tools in Microbial Biotechnology

235.9K

views

124

authors

22

articles

Editorial

07 December 2020

Editorial: Development and Application of Novel Genome Engineering Tools in Microbial Biotechnology

Jiazhang Lian

,

Yi Wang

,

Yunzi Luo

and

Chun Li

3,597 views

0 citations

Editors

4

Zhejiang University

University of California, Davis

Tianjin University

Tsinghua University

Impact

Fetching...

About

Over the past few years, novel genome engineering tools, i.e. ZFN,TALEN, CRISPR, MAGE and TRMR, have emerged and revolutionized our ability to modify microorganisms for both fundamental studies and biotechnological applications. More specifically, recent advances in genome engineering tools have enabled the assembly of multiple and/or large DNA fragments with high efficiency and fidelity, the optimization of biosynthetic pathways in a combinatorial and high throughput manner, and the evolution of microbial cells at an unprecedented speed and scale. Consequently, microorganisms have been extensively engineered to elucidate the microbial cellular machinery, to produce renewable fuels and chemicals at high titer, yield, and productivity, or to discover novel natural products as potential drugs.

This Research Topic focuses on the recent development and application of novel genome engineering tools, particularly the CRISPR systems, for both model (i.e. E. coli and S. cerevisiae) and non-model (i.e. P. pastoris and Clostridium species) microorganisms .Therefore, the proposed Research Topic is aimed to collect original research articles, short communications, reviews, mini-reviews, methods and perspectives at the leading edge of the microbial genome engineering field. As previously discussed, it is hoped that further advancements in this field of research will enable:

1. Efficient assembly and editing of big gene clusters and discovery of novel secondary metabolites (natural products)
2. Assembly and integration of multi-gene biosynthetic pathways
3. Multiplex and combinatorial genome engineering tools for the optimization of microbial cell factories
4. Genome-scale engineering for functional genomics and evolution of complex phenotypes

Over the past few years, novel genome engineering tools, i.e. ZFN,TALEN, CRISPR, MAGE and TRMR, have emerged and revolutionized our ability to modify microorganisms for both fundamental studies and biotechnological applications. More specifically, recent advances in genome engineering tools have enabled the assembly of multiple and/or large DNA fragments with high efficiency and fidelity, the optimization of biosynthetic pathways in a combinatorial and high throughput manner, and the evolution of microbial cells at an unprecedented speed and scale. Consequently, microorganisms have been extensively engineered to elucidate the microbial cellular machinery, to produce renewable fuels and chemicals at high titer, yield, and productivity, or to discover novel natural products as potential drugs.

This Research Topic focuses on the recent development and application of novel genome engineering tools, particularly the CRISPR systems, for both model (i.e. E. coli and S. cerevisiae) and non-model (i.e. P. pastoris and Clostridium species) microorganisms .Therefore, the proposed Research Topic is aimed to collect original research articles, short communications, reviews, mini-reviews, methods and perspectives at the leading edge of the microbial genome engineering field. As previously discussed, it is hoped that further advancements in this field of research will enable:

1. Efficient assembly and editing of big gene clusters and discovery of novel secondary metabolites (natural products)
2. Assembly and integration of multi-gene biosynthetic pathways
3. Multiplex and combinatorial genome engineering tools for the optimization of microbial cell factories
4. Genome-scale engineering for functional genomics and evolution of complex phenotypes

Download ebook

Share

Editors

Zhejiang University

University of California, Davis

Tianjin University

Tsinghua University

Impact

235,906 Total views

179,557 Article views

51,785 Article downloads

4,564 Topic views

Published In

Journal Thumbnail

Frontiers in Bioengineering and Biotechnology

Synthetic Biology

About Frontiers Research Topics

With their unique mixes of varied contributions from Original Research to Review Articles, Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author.

Suggest a topic