Cryopreservation has facilitated roles of biological research advancement through extending periods of cells storage. Though cryopreservation would render inactivation of cell metabolisms due to extremely low temperatures, cells enable to suffer insults during freezing and thawing processes. Among them, acting as damage factors, excessive generations of reactive oxygen species (ROS) and reactive nitrogen species (RNS) could injure basal functions and survival of cells.
As an adjunct to artificial assisted reproductive technologies, mammalian gamete and embryo cryopreservation has been widely used in medical, animal and agricultural sciences. However, compared to their fresh counterparts, the survival and development rates of gametes and embryos and their subsequent developmental competence and fertilizing capacity are significantly decreased after cryopreservation.
Oxidative and nitrosative stresses are common constraints during handling the in vitro mammalian gametes and embryos, which development is significantly affected by culture conditions; particularly by ROS and RNS, since their excess generation or absence could result in perturbation in the expression of key genes. Recent advancement in the development of protocols for sperm cryopreservation and in vitro maturation oocytes, fertilization, and embryo culture in Assisted reproduction techniques (ARTs) has provided the improved understanding of these processes. However, the search for developing a mutually beneficial procedure where ARTs could provide the optimal conditions for improved survival and development rates and clinical outcomes is crucial for forming efficient treatment strategies.
The study results of certain antioxidant agents’ applications are promising. However, according to the current body of evidence, it is necessary to carry out further researches, especially focusing on well-designed and larger randomized controlled studies, in purpose to minimize oxidative and nitrosative stress conditions during clinical ART setting.
The goal of this Research Topic is to highlight and collect cohesive research contributions related to the cryopreservation of mammalian gametes and embryos and the potential role of antioxidants in ameliorating oxidative and nitrosative stress, leading to improved development competence and fertilizing capacity.
This Special Issue aims to collate original research and review articles with a focus on the field of oocyte, embryo and sperm cryopreservation and their subsequent development both in vitro and in vivo.
· Influence of oxidative and nitrosative stress on gamete/embryo quality and the outcome of IVF/ICSI
· Oocyte cryopreservation and its impact on their in vitro maturation and development
· Ovary cryopreservation, sperm cryopreservation and embryo cryopreservation
· Implication of cryopreservation-inflicted oxidative stress in spermatogenesis, spermatogonial stem cells, mitosis, meiosis, and capacitation
· Role of antioxidants in ameliorating cryopreservation-inflicted damage in oocytes, embryos, and sperms
· Role of antioxidants in improving ART outcomes
Cryopreservation has facilitated roles of biological research advancement through extending periods of cells storage. Though cryopreservation would render inactivation of cell metabolisms due to extremely low temperatures, cells enable to suffer insults during freezing and thawing processes. Among them, acting as damage factors, excessive generations of reactive oxygen species (ROS) and reactive nitrogen species (RNS) could injure basal functions and survival of cells.
As an adjunct to artificial assisted reproductive technologies, mammalian gamete and embryo cryopreservation has been widely used in medical, animal and agricultural sciences. However, compared to their fresh counterparts, the survival and development rates of gametes and embryos and their subsequent developmental competence and fertilizing capacity are significantly decreased after cryopreservation.
Oxidative and nitrosative stresses are common constraints during handling the in vitro mammalian gametes and embryos, which development is significantly affected by culture conditions; particularly by ROS and RNS, since their excess generation or absence could result in perturbation in the expression of key genes. Recent advancement in the development of protocols for sperm cryopreservation and in vitro maturation oocytes, fertilization, and embryo culture in Assisted reproduction techniques (ARTs) has provided the improved understanding of these processes. However, the search for developing a mutually beneficial procedure where ARTs could provide the optimal conditions for improved survival and development rates and clinical outcomes is crucial for forming efficient treatment strategies.
The study results of certain antioxidant agents’ applications are promising. However, according to the current body of evidence, it is necessary to carry out further researches, especially focusing on well-designed and larger randomized controlled studies, in purpose to minimize oxidative and nitrosative stress conditions during clinical ART setting.
The goal of this Research Topic is to highlight and collect cohesive research contributions related to the cryopreservation of mammalian gametes and embryos and the potential role of antioxidants in ameliorating oxidative and nitrosative stress, leading to improved development competence and fertilizing capacity.
This Special Issue aims to collate original research and review articles with a focus on the field of oocyte, embryo and sperm cryopreservation and their subsequent development both in vitro and in vivo.
· Influence of oxidative and nitrosative stress on gamete/embryo quality and the outcome of IVF/ICSI
· Oocyte cryopreservation and its impact on their in vitro maturation and development
· Ovary cryopreservation, sperm cryopreservation and embryo cryopreservation
· Implication of cryopreservation-inflicted oxidative stress in spermatogenesis, spermatogonial stem cells, mitosis, meiosis, and capacitation
· Role of antioxidants in ameliorating cryopreservation-inflicted damage in oocytes, embryos, and sperms
· Role of antioxidants in improving ART outcomes
