About this Research Topic
Effective implementation of CRISPR has been pursued by delivery of a variety of combinations to target cells, including the complex of Cas9 protein and double-stranded crRNA/tracrRNA (known as ribonucleoproteins, or RNP), mRNA that expresses Cas9 in the target cell and double-stranded crRNA/tracrRNA, and a plasmid containing all the required nucleic acids together (including Cas9 nuclease expression cassette and a guide RNA cloning cassette). However, regardless of the specific approach, delivering the required CRISPR components into target cells faces many challenges that are shared with previously studied RNAi approach to protein silencing. An added complication is the delivery of multiple components with different physicochemical features. Recent promising results with CRISPR/Cas9 approach in a small population of patients with hereditary ATTR can open the doors to more effective clinical applications. A better understanding of different CRISPR/Cas9 approaches and challenges of effective delivery of CRISPR/Cas9 tools will enhance our chances of taking advantage of this powerful tool in translational and clinical research.
This collection aims to attract submissions focused (but not limited on) the following topics:
Different approaches to delivering CRISPR/Cas9 components
Recent accomplishments in CRISPR/Cas9 technology in animal models
Challenges for in vivo delivery of CRISPR/Cas9
CRISPR/Cas9 gene silencing vs. RNA interference
Synthetic vectors for delivery of CRISPR components
Viral vectors for delivery of CRISPR components
Note: Dr. Giedrius Gasiunas is an employee and co-founder of CasZyme.
Keywords: CRISPR/Cas9, Delivery, Vectors, Cancer, in vitro, Animal studies
Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.