DNA organization is of primary importance in any living cell, to conserve genetic information, achieve growth and pass it on to the next generation. DNA is organized at different levels, from local, DNA supercoiling and temporary short loops for gene regulation, to larger Chromosomal Interaction Domains (CIDs), and finally macrodomains, spanning a quarter of the chromosome. All these levels involve the intervention of a wide range of proteins like DNA topoisomerases, structural maintenance of chromosomes (SMCs) or nucleoid-associated proteins (NAPs). Understanding their concerted actions is of prime importance to understand how life can thrive in such a variety of environments.
Understanding how a cell organizes its DNA in assemblies that must be at once stable yet sufficiently dynamic to allow essential DNA-templated processes to occur is a key challenge. Recent advances in high-throughput and genome-wide techniques have generated a wealth of new data and helped new concepts and hypotheses to emerge. The goal of this research topic is to bring together new results and analyses from several disciplines including biochemistry, cell biology, microscopy, Hi-C/3C, ChIP and modeling that are now carried out on a variety of organisms to give an integrated view of DNA architecture and associated cellular processes. This topic will focus on Bacteria and Archaea, but comparisons with eukaryotes will be highly informative.
This research topic focuses on studies that investigate and discuss:
· Chromosome architecture
· DNA topology
· Chromosome organizing proteins
· Chromosome partitioning and segregation
· Modeling of DNA behaviour
DNA organization is of primary importance in any living cell, to conserve genetic information, achieve growth and pass it on to the next generation. DNA is organized at different levels, from local, DNA supercoiling and temporary short loops for gene regulation, to larger Chromosomal Interaction Domains (CIDs), and finally macrodomains, spanning a quarter of the chromosome. All these levels involve the intervention of a wide range of proteins like DNA topoisomerases, structural maintenance of chromosomes (SMCs) or nucleoid-associated proteins (NAPs). Understanding their concerted actions is of prime importance to understand how life can thrive in such a variety of environments.
Understanding how a cell organizes its DNA in assemblies that must be at once stable yet sufficiently dynamic to allow essential DNA-templated processes to occur is a key challenge. Recent advances in high-throughput and genome-wide techniques have generated a wealth of new data and helped new concepts and hypotheses to emerge. The goal of this research topic is to bring together new results and analyses from several disciplines including biochemistry, cell biology, microscopy, Hi-C/3C, ChIP and modeling that are now carried out on a variety of organisms to give an integrated view of DNA architecture and associated cellular processes. This topic will focus on Bacteria and Archaea, but comparisons with eukaryotes will be highly informative.
This research topic focuses on studies that investigate and discuss:
· Chromosome architecture
· DNA topology
· Chromosome organizing proteins
· Chromosome partitioning and segregation
· Modeling of DNA behaviour
