25.3K
views
46
authors
6
articles
Editors
4
Impact
Loading...

Plant-microbe interactions are a phenomenal display of symbiotic/parasitic relationships between living organisms. Plant growth-promoting rhizobacteria (PGPR) are some of the most widely investigated plant-beneficial microbes due to their capabilities in stimulating plant growth and development and conferring protection to plants against biotic and abiotic stresses. As such, PGPR-mediated plant priming/induced systemic resistance (ISR) has become a hot topic among researchers, particularly with prospects of applications in sustainable agriculture. The current study applies untargeted ultra-high performance liquid chromatography-high-definition mass spectrometry (UHPLC-HDMS) to investigate PGPR-based metabolic reconfigurations in the metabolome of primed wheat plants against Puccinia striiformis f. sp. tricti (Pst). A seed bio-priming approach was adopted, where seeds were coated with two PGPR strains namely Bacillus subtilis and Paenibacillus alvei (T22) and grown under controlled conditions in a glasshouse. The plants were infected with Pst one-week post-germination, followed by weekly harvesting of leaf material. Subsequent metabolite extraction was carried out for analysis on a UHPLC-HDMS system for data acquisition. The data was chemometrically processed to reveal the underlying trends and data structures as well as potential signatory biomarkers for priming against Pst. Results showed notable metabolic reprogramming in primary and secondary metabolism, where the amino acid and organic acid content of primed-control, primed-challenged and non-primed-challenged plants were differentially reprogrammed. Similar trends were observed from the secondary metabolism, in which primed plants (particularly primed-challenged) showed an up-regulation of phenolic compounds (flavonoids, hydroxycinnamic acids-HCAs- and HCA amides) compared to the non-primed plants. The metabolomics-based semi-quantitative and qualitative assessment of the plant metabolomes revealed a time-dependent metabolic reprogramming in primed-challenged and primed-unchallenged plants, indicating the metabolic adaptations of the plants to stripe rust infection over time.

3,919 views
13 citations
Recommended Research Topics
Frontiers Logo

Frontiers in Plant Science

Cross-Frontier Communication: Phytohormone Functions at the Plant-Microbe Interface and beyond
Edited by Susanne Berger, Saskia C M Van Wees, Ole Nybroe, Dominik K Großkinsky
163.8K
views
43
authors
9
articles
Frontiers Logo

Frontiers in Plant Science

Lipid Metabolism and Membrane Structure in Plant Biotic Interactions
Edited by Fiona L Goggin, Jyoti Shah, Glenda E Gillaspy
20.9K
views
34
authors
6
articles
Frontiers Logo

Frontiers in Plant Science

The Potential of Omics for Understanding and Managing Plant Diseases
Edited by Pallem Chowdappa, Rajesh MK
18.3K
views
51
authors
7
articles
Frontiers Logo

Frontiers in Plant Science

Plant-parasitic Nematode and Plant Interaction under Abiotic and Biotic Stresses
Edited by Chen Jingsheng, Yanfeng Hu, Guiping Yan, Xiaoli Guo
16.4K
views
51
authors
7
articles
Frontiers Logo

Frontiers in Plant Science

Innovative Strategies for Enhancing Plant Resilience to Phytopathogenic Microbes
Edited by Yasser Nehela, Temoor Ahmed, Dirk Janssen
17.7K
views
97
authors
11
articles