About this Research Topic
The goal of this Research Topic is to provide a succinct and perspicuous guide to the different techniques deployed for the directed evolution of enzymes. Besides illustrating the usage of various state-of-the-art methodologies, aimed to increase the thermo-/ph-stability, activity, and enantiomeric affinity of an enzyme, the collection will provide an updated record of basic principles involved in designing a directed evolution experiment and the associated industrial and/or commercial applications.
This Research Topic welcomes submissions that exemplarily discuss various steps of the enzyme design approaches, taking into account industrial applications and viewpoints of end-users, and will cover themes such as, but not limited to:
• Screening methodologies to identify the most effective target enzymes
• Protein engineering techniques to functionally improve the active site
• Design techniques for tuning the active site against natural/non-natural substrate(s) to improve the productivity
• Overexpression of the target enzymes using the customized experimental methods
• Refining the reaction mechanism and bioprocesses to overcome rate-limiting steps
Keywords: Directed evolution, enzyme design, protein engineering, rational design, bioproduction, catalytic improvement, sequence space, screening and selection
Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.