Innate Immunity Programming and Memory in Resolving and Non-Resolving Inflammation

Editorial

11 February 2020

Editorial: Innate Immunity Programming and Memory in Resolving and Non-Resolving Inflammation

Liwu Li

Charles McCall

and

Xiaoyu Hu

3,141 views

4 citations

Editors

Liwu Li

Virginia Tech

Charles E McCall

Wake Forest Baptist Medical Center

Xiaoyu Hu

Tsinghua University

Impact

Original Research

30 April 2018

Bacillus Calmette–Guérin-Induced Trained Immunity Is Not Protective for Experimental Influenza A/Anhui/1/2013 (H7N9) Infection in Mice

L. Charlotte J. de Bree

, 10 more and

John Dulos

12,353 views

32 citations

IL-17A, IL-17F, CXCL8, and IL-1ra mRNA expression levels in human neutrophils activated by a variety of stimuli. Human neutrophils were cultured at 5 × 106/ml for up to 20 h with (A) 100 U/ml IFNγ and/or 100 ng/ml LPS; (B) 1,000 U/ml IFNα and/or 5 µM R848; (C) 10 ng/ml GM-CSF or 100 nM fMLF; (D) 1,000 U/ml G-CSF or 5 ng/ml TNFα. IL-17A, IL-17F, CXCL8, and IL-1ra mRNA expression was evaluated by reverse transcription quantitative real-time PCR (RT-qPCR) and data depicted as mean normalized expression (MNE) units after GAPDH mRNA normalization. The experiments depicted in each panels (A–D) are representative of at least three ones with similar results. Error bars stand for SEs calculated from triplicate qPCR reactions.

Original Research

17 April 2018

A Reappraisal on the Potential Ability of Human Neutrophils to Express and Produce IL-17 Family Members In Vitro: Failure to Reproducibly Detect It

Nicola Tamassia

, 10 more and

Marco A. Cassatella

8,763 views

48 citations

Depleting sirtuin 4 (SIRT4) hampers recovery of PDC inhibition by increasing PDK1 expression during physiological resolution of acute inflammatory response. Control and SIRT4 knockdown or control and SIRT4 knockout THP-1 cells were stimulated with 1 µg/ml lipopolysaccharide (LPS). (A) PDC decarboxylase activity was evaluated by commercial colorimetric assay kit (N = 3). (B) PDK1 mRNA transcription was measured using real-time RT-PCR. PDK1 protein levels (C) and PDC protein levels and S232 phosphorylated PDC E1α levels (D) were shown by immunoblotting. Bar graphs depict mean values ± SE of N = 3. PDC, pyruvate dehydrogenase complex, PDK1, pyruvate dehydrogenase kinase 1, GAPDH, glyceraldehyde 3-phosphate dehydrogenase, KD, knockdown, KO, knockout.

Original Research

09 March 2018

Mitochondrial Sirtuin 4 Resolves Immune Tolerance in Monocytes by Rebalancing Glycolysis and Glucose Oxidation Homeostasis

Jie Tao

, 3 more and

Tie Fu Liu

8,279 views

67 citations

Changes in Sonic hedgehog (SHH) expression by bone marrow cells following systemic E. coli infection. Saline: i.v. injection of saline; E. coli: i.v. injection of E. coli; MCF, mean channel fluorescent intensity. Data are presented as mean ± SEM. N = 5. Bars with different letters in each panel are statically different (p < 0.05).

Original Research

26 February 2018

Sonic Hedgehog Signaling Regulates Hematopoietic Stem/Progenitor Cell Activation during the Granulopoietic Response to Systemic Bacterial Infection

Xin Shi

, 4 more and

Ping Zhang

4,096 views

15 citations

Comparison of H3K4me3-related GO-terms in β-trained monocytes and monocytes of SLE patients.

Review

20 February 2018

The Potential Role of Trained Immunity in Autoimmune and Autoinflammatory Disorders

Rob J. W. Arts

, 1 more and

Mihai G. Netea

19,039 views

146 citations

MRictor−/− macrophages have increased levels of inflammatory gene expression in response to lipopolysaccharide (LPS) and a low dose of Akt inhibitor increases the LPS-induced expression of inflammatory genes in Rictorfl/fl but not in MRaptor−/− macrophages. (A–D) Peritoneal macrophages were incubated with media alone (control) or together with LPS (20 ng/ml) for 5 h and the gene-expression levels were measured by real-time polymerase chain reaction. Note MRictor−/− macrophages have increased pro-inflammatory and decreased of interleukin-10 gene expression. Graphs represent data (mean ± SEM) obtained from the same numbers (n = 3 per group) of mice (*p < 0.05 and **p < 0.001 compared to Rictorfl/fl cells treated with LPS by t-test). (E–J) Peritoneal macrophages were isolated from Rictorfl/fl and MRaptor−/− mice and 2 days later were treated with media (Control), with the Akt inhibitor IV (31 μM, Inh) or LPS (20 ng/ml) alone or together with the inhibitor (LPS + Inh) for 5 h at 37°C and the gene-expression levels were measured by real-time polymerase chain reaction. Note a low dose of the Akt inhibitor increases LPS-mediated inflammatory gene expression but loss of Raptor reverses the increase. Graphs represent data (mean ± SEM) obtained from the same numbers (n = 3 per group) of mice (*p < 0.05 and **p < 0.001 compared to WT cells treated with LPS by t-test test).

Original Research

13 February 2018

Loss of Rictor in Monocyte/Macrophages Suppresses Their Proliferation and Viability Reducing Atherosclerosis in LDLR Null Mice

Vladimir R. Babaev

, 4 more and

MacRae F. Linton

5,049 views

19 citations

Review

05 February 2018

A Role for the Krebs Cycle Intermediate Citrate in Metabolic Reprogramming in Innate Immunity and Inflammation

Niamh C. Williams

and

Luke A. J. O’Neill

Metabolism in immune cells is no longer thought of as merely a process for adenosine triphosphate (ATP) production, biosynthesis, and catabolism. The reprogramming of metabolic pathways upon activation is also for the production of metabolites that can act as immune signaling molecules. Activated dendritic cells (DCs) and macrophages have an altered Krebs cycle, one consequence of which is the accumulation of both citrate and succinate. Citrate is exported from the mitochondria via the mitochondrial citrate- carrier. Cytosolic metabolism of citrate to acetyl-coenzyme A (acetyl-CoA) is important for both fatty-acid synthesis and protein acetylation, both of which have been linked to macrophage and DC activation. Citrate-derived itaconate has a direct antibacterial effect and also has been shown to act as an anti-inflammatory agent, inhibiting succinate dehydrogenase. These findings identify citrate as an important metabolite for macrophage and DC effector function.

125,086 views

458 citations