Research Topic

Biophysical Target Engagement Assays in Chemical Biology and Pharmacological Research

About this Research Topic

Biophysical methods for the detection and quantification of binding between small molecules or biologicals and their target are fundamental to chemical biology and pharmacological research. Target engagement assays can be utilized for target deconvolution and validation, establishing structure-activity relationships, probe discovery, and understanding the mode of action of prospective therapeutics.


Different biophysical methods such as isothermal titration calorimetry, differential scanning fluorimetry, surface plasmon resonance, biolayer interferometry and microscale thermophoresis have been established to measure target-ligand interactions using purified target molecules. Despite their various advantages each method also has different limitations, including high material consumption, sensitivity limits, low throughput or impacted performance when working with complex matrices or crude material. Moreover, the binding observed between a ligand and a target in a cell-free system does not always translate to a similar interaction within the intracellular environment. Therefore, new techniques have emerged in recent years to quantify the binding of small molecules and biologicals to targets within living cells.


The goal of the current Research Topic is to introduce novel approaches to monitor and measure target engagement, and present new technological and methodological advances to break through the limitations of established methods in cell free and living systems.


The scope of this Research Topic is to cover the application of well established, recent, and novel biophysical target engagement technologies in chemical biology and pharmacological research. We welcome all available article types, including original research articles, brief research reports, and reviews covering, but not limited to:
• Target engagement in cells
• Cell free assays
• Target deconvolution
• Mode of action studies
• Labelled chemical probes


Keywords: molecular interaction, target engagement, structure-activity relationship, mode of action, binding kinetics, cellular assay


Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.

Biophysical methods for the detection and quantification of binding between small molecules or biologicals and their target are fundamental to chemical biology and pharmacological research. Target engagement assays can be utilized for target deconvolution and validation, establishing structure-activity relationships, probe discovery, and understanding the mode of action of prospective therapeutics.


Different biophysical methods such as isothermal titration calorimetry, differential scanning fluorimetry, surface plasmon resonance, biolayer interferometry and microscale thermophoresis have been established to measure target-ligand interactions using purified target molecules. Despite their various advantages each method also has different limitations, including high material consumption, sensitivity limits, low throughput or impacted performance when working with complex matrices or crude material. Moreover, the binding observed between a ligand and a target in a cell-free system does not always translate to a similar interaction within the intracellular environment. Therefore, new techniques have emerged in recent years to quantify the binding of small molecules and biologicals to targets within living cells.


The goal of the current Research Topic is to introduce novel approaches to monitor and measure target engagement, and present new technological and methodological advances to break through the limitations of established methods in cell free and living systems.


The scope of this Research Topic is to cover the application of well established, recent, and novel biophysical target engagement technologies in chemical biology and pharmacological research. We welcome all available article types, including original research articles, brief research reports, and reviews covering, but not limited to:
• Target engagement in cells
• Cell free assays
• Target deconvolution
• Mode of action studies
• Labelled chemical probes


Keywords: molecular interaction, target engagement, structure-activity relationship, mode of action, binding kinetics, cellular assay


Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.

About Frontiers Research Topics

With their unique mixes of varied contributions from Original Research to Review Articles, Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author.

Topic Editors

Loading..

Submission Deadlines

01 October 2021 Abstract
01 February 2022 Manuscript

Participating Journals

Manuscripts can be submitted to this Research Topic via the following journals:

Loading..

Topic Editors

Loading..

Submission Deadlines

01 October 2021 Abstract
01 February 2022 Manuscript

Participating Journals

Manuscripts can be submitted to this Research Topic via the following journals:

Loading..
Loading..

total views article views article downloads topic views

}
 
Top countries
Top referring sites
Loading..