Microbial cell factory (MCF) is about engineered microorganisms that can efficiently convert low-value substrates into valuable products. Due to the eco-friendly process of bioconversion, MCF has been considered a green biological manufacturing technology to substitute traditional chemical industry. In particular, the intrinsic metabolic routes for target chemical synthesis could be artificially designed, created, and rewired to maximum flux. For example, MCF can utilize one carbon compound (CO2, methanol, formate, and methane) as a sole substrate to produce complicated multi-carbon compounds, which indicates the attractive and impressive potential of carbon neutralization. Those metabolic routes should depend on cofactors (such as NAD(P)H and its precursor, FADH2, ATP, coenzyme A, and other cofactors) to drive the extension of carbon chains. However, inherent cofactor regeneration is low efficiency, and it needs engineered strategies to force the regeneration. Nevertheless, high regeneration speed also results in cofactor ratio imbalance that decreases target chemical accumulation and even cell death.
In this Research Topic, we would like to collect cofactor regenerating mechanisms and advanced cofactor regeneration technologies, including FDH (formate dehydrogenase)-dependent strategy, route compensation, photo/electron-driven strategy, to argue MCF bioproduction.
We welcome original research articles, perspectives, and reviews highlighting the recent progress in all aspects of cofactor regeneration technologies in MCF. Potential topics include but are not limited to the following:
1. Cofactor regeneration mechanisms of either wild-type or engineered MCF.
2. Biosensors or riboswitches for cofactor regeneration.
3. Cofactor engineering in MCF for valuable chemical synthesis.
4. Strategies for augmenting cofactor regenerating efficiency.
5. MCF for one-carbon compounds conversion.
6. Photo/electron-driven intracellular cofactor regeneration.
Microbial cell factory (MCF) is about engineered microorganisms that can efficiently convert low-value substrates into valuable products. Due to the eco-friendly process of bioconversion, MCF has been considered a green biological manufacturing technology to substitute traditional chemical industry. In particular, the intrinsic metabolic routes for target chemical synthesis could be artificially designed, created, and rewired to maximum flux. For example, MCF can utilize one carbon compound (CO2, methanol, formate, and methane) as a sole substrate to produce complicated multi-carbon compounds, which indicates the attractive and impressive potential of carbon neutralization. Those metabolic routes should depend on cofactors (such as NAD(P)H and its precursor, FADH2, ATP, coenzyme A, and other cofactors) to drive the extension of carbon chains. However, inherent cofactor regeneration is low efficiency, and it needs engineered strategies to force the regeneration. Nevertheless, high regeneration speed also results in cofactor ratio imbalance that decreases target chemical accumulation and even cell death.
In this Research Topic, we would like to collect cofactor regenerating mechanisms and advanced cofactor regeneration technologies, including FDH (formate dehydrogenase)-dependent strategy, route compensation, photo/electron-driven strategy, to argue MCF bioproduction.
We welcome original research articles, perspectives, and reviews highlighting the recent progress in all aspects of cofactor regeneration technologies in MCF. Potential topics include but are not limited to the following:
1. Cofactor regeneration mechanisms of either wild-type or engineered MCF.
2. Biosensors or riboswitches for cofactor regeneration.
3. Cofactor engineering in MCF for valuable chemical synthesis.
4. Strategies for augmenting cofactor regenerating efficiency.
5. MCF for one-carbon compounds conversion.
6. Photo/electron-driven intracellular cofactor regeneration.
