About this Research Topic
In recent years, we have observed a revolution in taxonomy and diagnosis of human diseases due to the advent of the next-generation sequencing technology. Long lists of potential disease contributors have been accumulated, which provides an unprecedented opportunity to impact on the outcome of many human diseases, albeit requiring a more in-depth analysis of genotype-to-phenotype relationships. Correlation does not imply causation and specific molecular factors need to be modeled thoroughly in order to dissect out mechanisms that can be then targeted for effective therapies. Weighing individual contributions in complex multigenic diseases can be difficult in whole-animal models. The next-generation of models, including organoids and iPSCs, represents the new frontier of cancer modeling that offers dynamic platforms for fit-for purpose perturbation analysis in order to link specific molecular events to the development of the disease.
This Research Topic on “Modeling Human Diseases Using Next-Generation Cell Culture Systems” aims at delivering a high-level overview on methodologies and experimental approaches based on the use of organoid and iPSC culture systems to understand mechanisms of disease, including cancer. These breakthrough technologies are relatively new and there is an opportunity to refine methodologies and define robust metrics for appropriate use of the models, which will certainly enhance our understanding of human diseases. In order to attain that objective, we aim at collecting original researches or reviews from young and well-established scientists around the globe.
Keywords: Disease Model, Organoid, iPSC, Cell Culture, Model Systems