Recent years have seen incredible integration of technology in biology. Understanding and translation of human cellular biology is benefiting from technological breakthroughs in 3D bioprinting and organoidogenesis paired with advanced cell and tissue analytics. The tissues generated better recapitulate in vivo cell-systems in vitro, with high-throughput and automated molecular and cellular screening, demonstrated by RNA sequencing and quantitative whole mount high-content imaging, seamlessly embedded within laboratory workflows for foundational research and clinical practice. A range of bioengineering technologies, such as advanced biomaterials and microfluidics multiplexed with systems for transcriptomics and proteomics, are further driving this innovation through better, controlled, and scrutinised tissue building, inclusive of spatially organised extracellular matrix and more relevant multicellular phenotypes.
In tissue engineering, the preservation of cell-cell and cell-extracellular matrix contacts within a 3D environment is fundamental for better recapitulating native tissues. For example, tailoring the extracellular micro-environment via mechanical, topographical, and molecular factors allows guidance and support of cell behavior. Although such improvements in tissue and organ modeling have enabled new opportunities for drug screening and disease modeling, there remains a need for new strategies to further innovate tissues in terms of size and complexity and more widely implement advanced platforms for cell and tissue phenotyping.
The scope of this Research Topic is to collect works that highlight the most recent advances for developing high-fidelity in vitro human tissue and organ models as well as advanced technologies for their analysis. As a multifaceted topic, sub-categories of interest include, but are not limited to, advanced printer systems and other platforms for constructing, controlling and analysing the spatiotemporal arrangement of cellular phenotypes within high-level assemblies, extending to enablers of functional extracellular soluble and structural microenvironments. Complex in vitro cell/tissue/organ systems include multicellular organoids, microtissues, and organotypic cultures, and methodologies to produce the extracellular environment may include natural and synthetic biomaterials.
Image: 3D rendering analysis of a 3D bioprinted vascularized cardiac spheroid, courtesy of Dr Carmine Gentile, UTS.
Recent years have seen incredible integration of technology in biology. Understanding and translation of human cellular biology is benefiting from technological breakthroughs in 3D bioprinting and organoidogenesis paired with advanced cell and tissue analytics. The tissues generated better recapitulate in vivo cell-systems in vitro, with high-throughput and automated molecular and cellular screening, demonstrated by RNA sequencing and quantitative whole mount high-content imaging, seamlessly embedded within laboratory workflows for foundational research and clinical practice. A range of bioengineering technologies, such as advanced biomaterials and microfluidics multiplexed with systems for transcriptomics and proteomics, are further driving this innovation through better, controlled, and scrutinised tissue building, inclusive of spatially organised extracellular matrix and more relevant multicellular phenotypes.
In tissue engineering, the preservation of cell-cell and cell-extracellular matrix contacts within a 3D environment is fundamental for better recapitulating native tissues. For example, tailoring the extracellular micro-environment via mechanical, topographical, and molecular factors allows guidance and support of cell behavior. Although such improvements in tissue and organ modeling have enabled new opportunities for drug screening and disease modeling, there remains a need for new strategies to further innovate tissues in terms of size and complexity and more widely implement advanced platforms for cell and tissue phenotyping.
The scope of this Research Topic is to collect works that highlight the most recent advances for developing high-fidelity in vitro human tissue and organ models as well as advanced technologies for their analysis. As a multifaceted topic, sub-categories of interest include, but are not limited to, advanced printer systems and other platforms for constructing, controlling and analysing the spatiotemporal arrangement of cellular phenotypes within high-level assemblies, extending to enablers of functional extracellular soluble and structural microenvironments. Complex in vitro cell/tissue/organ systems include multicellular organoids, microtissues, and organotypic cultures, and methodologies to produce the extracellular environment may include natural and synthetic biomaterials.
Image: 3D rendering analysis of a 3D bioprinted vascularized cardiac spheroid, courtesy of Dr Carmine Gentile, UTS.
