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MINI REVIEW article

Front. Immunol., 26 December 2013
Sec. Inflammation
This article is part of the Research Topic Carbohydrates: the yet to be tasted sweet spot of immunity View all 10 articles

Heparanase and autoimmune diabetes

  • 1Diabetes/Transplantation Immunobiology Laboratory, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
  • 2Cancer and Vascular Biology Group, Department of Immunology, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia

Heparanase (Hpse) is the only known mammalian endo-β-d-glucuronidase that degrades the glycosaminoglycan heparan sulfate (HS), found attached to the core proteins of heparan sulfate proteoglycans (HSPGs). Hpse plays a homeostatic role in regulating the turnover of cell-associated HS and also degrades extracellular HS in basement membranes (BMs) and the extracellular matrix (ECM), where HSPGs function as a barrier to cell migration. Secreted Hpse is harnessed by leukocytes to facilitate their migration from the blood to sites of inflammation. In the non-obese diabetic (NOD) model of autoimmune Type 1 diabetes (T1D), Hpse is also used by insulitis leukocytes to solubilize the islet BM to enable intra-islet entry of leukocytes and to degrade intracellular HS, an essential component for the survival of insulin-producing islet beta cells. Treatment of pre-diabetic adult NOD mice with the Hpse inhibitor PI-88 significantly reduced the incidence of T1D by ~50% and preserved islet HS. Hpse therefore acts as a novel immune effector mechanism in T1D. Our studies have identified T1D as a Hpse-dependent disease and Hpse inhibitors as novel therapeutics for preventing T1D progression and possibly the development of T1D vascular complications.

Introduction

Heparanase (Hpse) is an endo-β-d-glucuronidase that degrades the glycosaminoglycan heparan sulfate (HS). Cloning studies have identified that catalytically active Hpse is encoded by a single mammalian gene (13). Hpse is initially produced as an inactive pre-proenzyme which undergoes post-translational processing to yield a 65 kDa proenzyme for secretion. Proteolytic cleavage of proheparanase by the cysteine protease cathepsin L leads to the formation of catalytically active Hpse, a heterodimer consisting of a 50 kDa (human) or 48 kDa (mouse) polypeptide non-covalently bound to a 8 kDa peptide (1, 2, 48). HS is a linear polysaccharide that consists of a repeating disaccharide composed of N-acetylated glucosamine (GlcNAc) and uronic acid [glucuronic acid (GlcA) or iduronic acid (IdoA)]. HS biosynthesis occurs in the Golgi compartment of cells, with the assembly of component sugar residues occurring directly onto the core proteins of heparan sulfate proteoglycans (HSPGs) (911). During the polymerization of HS chains, selected sugar residues are chemically modified by a suite of enzymes (N-deacetylase-N-sulfotranferase, C5 epimerase, and 2, 3, and 6-O-sulfotransferases), resulting in HS chains with regions that are highly sulfated and other regions of lower or no sulfation (10, 11). The sulfated regions of HS, in particular, bind to a vast array of bioactive ligands that include cytokines, chemokines, growth factors, adhesion molecules, lipases, and proteases (12, 13).

Typically, HSPGs are localized at the cell surface (e.g., syndecans 1–4, glypicans 1–6), in the extracellular matrix (ECM), in basement membranes (BMs) (e.g., perlecan, collagen type XVIII, and agrin) and have been identified in the nucleus of certain cells (14, 15). Secreted proheparanase rapidly interacts with cell surface HSPGs and the proheparanase-HSPG complex subsequently undergoes endocytosis. Similarly, Hpse can be internalized after binding to cell surface lipoprotein receptor-related proteins (LPRs) and mannose-6-phosphate receptors (MPRs) (16). Internalized proheparanase is cleaved by intracellular cathepsin L at acidic pH in late endosomes or lysosomes, to form catalytically active Hpse which can either degrade co-endocytosed HS, thereby regulating the turnover of cell-associated HS, or undergo storage within the lysosomes for subsequent secretion (6, 1721). Optimal Hpse-mediated cleavage of glycosidic bonds in HS occurs at pH 5.5–6.0 and typically at sites adjacent to N- or 6-O-sulfated glucosamine (16, 22), e.g., the linkage of glucuronic acid to 6-O-sulfated glucosamine (23). HS in BMs and ECM is degraded by Hpse secreted by platelets, endothelial cells, leukocytes, and metastasizing tumor cells (12). In these settings, Hpse activity can result from (i) activation of proheparanase bound to cell surface HSPG or to cation-independent MPRs (CIMPRs) by an extracellular source of cathepsin L, e.g., produced by macrophages (24, 25); (ii) cytokine-, fatty acid-, or nucleotide-stimulated release of an intracellular pool of catalytically active Hpse (2629) which may be subsequently captured by cell surface receptors such as CIMPRs (25); or (iii) platelet degranulation (30). This regulated release of Hpse in the local microenvironment limits the availability of Hpse activity, preserving the essential and diverse biological functions of HS.

Heparanase also exhibits non-enzymatic functions which impact on cell signaling, adhesion, and migration, as well as on gene expression. Such functions are generally expressed at neutral pH (3133). Interaction of Hpse with cell surface receptors on endothelial cells activates intracellular Akt, PI3K, and p38 kinase signaling to stimulate cell migration and Src kinase-mediated upregulation of vascular endothelial growth factor (VEGF) for angiogenesis (6, 18, 34). Hpse lacking catalytic enzyme activity has been shown to increase the expression of certain growth factors (35) and to facilitate cell binding to HS in the ECM and to endothelial cells in vitro (32).

Intra-nuclear Hpse modulates intra-nuclear HS/HSPGs and exerts direct effects on gene transcription. Transfer of Hpse to the nucleus occurs via Hsp90 in endothelial cells following fatty acid stimulation (29). Intra-nuclear Hpse decreases the level of the HSPG syndecan-1 in the nucleus of myeloma cells (14) and cleaves nuclear HS which in turn inhibits histone acetyltransferases (36). Recently, active Hpse has been reported to directly mediate epigenetic effects by regulating histone methylation, a process that directly influences the transcription of certain immune response genes involved in T-cell migration and function, e.g., IL-2 and IFN-γ (37). Hpse was also found to bind to the promoters of micro-RNAs involved in T-cell differentiation (37) and to influence the transcription of genes encoding enzymes involved in glucose metabolism (29). Such nuclear roles for Hpse, either with or without HS-degrading activity, would be expected to impact on T cells in inflammatory responses.

Heparanase and Inflammation

Heparan sulfate has several important biological functions which are regulated by Hpse in inflammation. An inflammatory response is generated when leukocytes are rapidly recruited from the blood to sites of tissue injury. In the early stages of inflammation, cell surface HS on cytokine-activated or inflamed endothelial cells functions in presenting lymphocyte-attractant chemokines to leukocytes in the vascular lumen (12, 38). The subsequent immobilization of the leukocytes (e.g., T cells) at the endothelial cell surface is enhanced by the binding of chemokine-activated integrins on the leukocytes to adhesion molecules such as ICAM-1 or VCAM-1 expressed on endothelial cells. Such interactions could potentially be facilitated by the binding of T cell-bound inactive Hpse to HS expressed on the surface of endothelial cells (12, 32, 33). The chemokine-binding role for endothelial cell surface HS may also function in establishing a chemokine gradient to direct leukocyte migration across the endothelium (12). Having crossed the blood vessel wall, most probably by passing between endothelial cells, inflammatory leukocytes employ degradative mechanisms to traverse the sub-endothelial BM. In fact BM HS, particularly associated with the HSPG perlecan, helps the BM to act as a barrier to leukocyte migration. This barrier property is attributed to the length of HS chains (up to 400 sugar residues) and to their intrinsic capacity to interact with other BM matrix proteins, forming a cell-impenetrable scaffold (12). To overcome this obstacle, leukocytes including T cells (39, 40), nearby endothelial cells (26) and possibly platelets (40) produce Hpse to degrade BM HS and proteases to destroy BM matrix proteins. The disassembly of the BM matrix components aids the passage of leukocytes across the BM and their entry into the surrounding tissue. Similarly, Hpse is released by inflammatory leukocytes to solubilize HS in the ECM of underlying tissues and to aid their navigation to sites of inflammation (12). During the course of the degradation of extracellular HS, HS-bound cytokines and chemokines can be liberated into the local microenvironment, potentially augmenting cell recruitment and exacerbating the inflammatory response (12).

The role for Hpse as a “path-maker” required by migrating leukocytes is of particular significance for T cell-mediated autoimmune diseases. Indeed, Hpse activity represents a prime target for anti-inflammatory drug development. Experimental autoimmune encephalitis (EAE; an experimental model of multiple sclerosis) in rats was largely prevented by in vivo treatment with sulfated polysaccharides. This effect was attributed to the inhibition of Hpse produced by activated T cells, which in turn blocked the solubilization of the sub-endothelial BM (41, 42). In a delayed-type hypersensitivity (DTH) experimental model of inflammation, inhibition of endothelial cell-derived Hpse prevented the degradation of sub-endothelial BM HS and leukocyte migration (27). Hpse, possibly produced by inflammatory cells in rheumatoid arthritis in humans, may release cytokines and/or chemokines from degraded HS in the ECM of rheumatoid joints, promoting joint destruction (43). In ulcerative colitis and Crohn’s disease, which represent chronic inflammatory disorders, Hpse is preferentially produced by inflamed gut epithelial cells to drive a local circuit of inflammation (24, 44). A role for Hpse has therefore been established in a broad range of inflammatory conditions.

Heparanase and the Pathogenesis of Type 1 Diabetes

Type 1 diabetes is an autoimmune disease which has been extensively studied in non-obese diabetic (NOD) mice, a recognized preclinical model of T1D in humans. During T1D, the insulin-producing beta cells in the islets of Langerhans in the pancreas are selectively destroyed by a T cell-mediated autoimmune response (45). The priming of autoreactive T cells to their cognate beta cell-specific autoantigens most probably occurs in the draining pancreatic lymph nodes, possibly as a consequence of both the abnormal responsiveness of effector T cells and inadequate control by regulatory T cells (46). Histological studies of NOD female mice at an early age (~6–7 weeks) revealed that leukocytes initially accumulate around the periphery of the islets, forming foci of non-destructive inflammation (insulitis). In adult pre-diabetic mice, the insulitis advances to a destructive phenotype, with peri-islet inflammatory leukocytes invading the islet cell mass (45). However, this pathology does not occur as a synchronized process throughout the pancreas, and the proportion of affected islets as well as the severity of leukocyte invasion progressively increases with time. Clinical symptoms of T1D are observed in 60–80% of female NOD mice from ~100 days of age or older, and are characterized by blood glucose levels exceeding >20 mmol/L (hyperglycemia).

In view of the established role for Hpse in leukocyte migration in other experimental models of inflammation (see above), we predicted that during T1D development, Hpse produced by islet beta cell-specific autoreactive T cells, inflammatory leukocytes, and possibly endothelial cells in the pancreatic vasculature, would be required to degrade HS in the sub-vascular endothelial BM. Thereafter, Hpse-mediated degradation of HS in the underlying pancreatic ECM would allow the inflammatory cells to migrate to individual islets and destroy the islet beta cells. Since T1D development is a chronic disease process, we furthermore speculated that there would be an on-going need for this degradative activity. Our studies identified, however, that the requirement for Hpse extended far beyond the enzymatic activity necessary for leukocyte migration and the establishment of chronic inflammation. Indeed we discovered a critical role for Hpse at the level of the islets themselves. This local involvement of Hpse stemmed from the exceptionally high levels of HS normally associated with the islets in situ (8). Initially we confirmed the presence of a BM at the islet periphery (i.e., peri-islet BM) and revealed the HSPG perlecan as a previously unrecognized constituent (47). This HS +ve islet BM was predicted to act as a barrier to invading cells, analogous to the sub-endothelial BM. On further investigation of the distribution of HS in normal mouse islets in situ, we found that HS was expressed not only in the islet BM but at extraordinarily high levels throughout the islet cell mass (8). Immunohistochemical studies demonstrated that insulitis mononuclear cells (MNCs) in NOD mice strongly expressed cell surface Hpse (Figure 1; Figure 2, Stage 1). Furthermore, Western blotting analyses showed that the insulitis leukocytes expressed high levels of catalytically active Hpse at the time of diabetes onset in NOD mice, in contrast to the expression of inactive Hpse by peri-islet leukocytes in young pre-diabetic mice (8). Intra-islet infiltration by insulitis MNCs correlated with disruption of the islet BM (Figure 2, Stage 2), loss of the islet BM matrix proteins including the HSPG perlecan (47), progressive loss of intra-islet HS (Figure 2, Stage 3) and beta cell death (Figure 2, Stage 4) (8). Our studies have strongly indicated that such processes are mediated by catalytically active Hpse (Figure 2, Stages 2–4). This newly unveiled role for Hpse has more recently been validated using a transgenic mouse model of acute T1D and adoptively transferred Hpse-knockout effector T cells (unpublished data).

FIGURE 1
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Figure 1. Autoimmune T1D in NOD/Lt mice is characterized by cell surface expression of heparanase on insulitis leukocytes. Histology (A) and immunohistochemistry (B) of a pancreatic islet in a NOD/Lt female mouse at the time of T1D onset shows islet infiltration by destructive heparanase (Hpse)-expressing insulitis leukocytes (B) localized particularly at the insulitis-islet interface, a process which leads to loss of beta cell HS and beta cell death. (A) hematoxylin and eosin; (B) HP130 anti-heparanase mAb. Ins, insulitis mononuclear cells; DI, damaged islet tissue; Hpse, heparanase.

FIGURE 2
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Figure 2. Diagram showing a pancreatic islet and four stages of T1D disease driven by heparanase and loss of intra-islet HS. HS (intense blue color) is shown in normal beta cells and in intact peri-islet BM. In Stage 1 of the disease process, non-destructive insulitis mononuclear cells produce heparanase (red dots). Onset of destructive insulitis occurs when heparanase becomes catalytically active and degrades HS in the islet BM (Stage 2). Damage to the islet BM barrier allows activated autoreactive T cells to enter the islet cell mass where the local production of heparanase leads to degradation of intracellular HS in islet beta cells (paling blue color; Stage 3). Progression of HS depletion throughout the islet beta cell population results in increased beta cell death (palest blue color in “shriveled” beta cells; Stage 4), loss of insulin production and ultimately the development of T1D. MNC, mononuclear cell; BM, basement membrane; Hpse, heparanase.

In vitro studies of beta cells isolated from normal mouse islets revealed both the unique intracellular localization of HS and its function in maintaining the viability of beta cells (8). Loss of intracellular HS correlated with beta cell death and conversely, the restoration of intracellular HS after culture of the beta cells with HS mimetics, correlated with beta cell survival. HS replacement not only preserved beta cell viability but rendered the beta cells resistant to oxidative damage induced by treatment with hydrogen peroxide [a source of reactive oxygen species (ROS)] (8). Collectively these findings led us to speculate that the intrinsic role of intracellular beta cell HS in situ in the pancreas is to protect the beta cells from the physiological levels of ROS generated as a consequence of their high metabolic and biosynthetic activity (8). Furthermore, we reasoned that such a mechanism could compensate for the low levels of free radical scavenger enzymes in beta cells (48).

Together, our in vivo and in vitro studies identified multiple roles for Hpse in T1D, namely promoting the migration of leukocytes from pancreatic blood vessels (i.e., across the sub-endothelial BM and through the pancreatic ECM), aiding the passage of leukocytes across the islet BM and depleting islet beta cells of the intracellular HS needed for their survival (Figure 2). In support of this new paradigm, in vivo treatment of pre-diabetic adult NOD female mice with the Hpse inhibitor/HS mimetic, PI-88, for 180 days significantly delayed T1D onset and reduced the incidence of diabetes by ~50% (8). Compared to saline-treated control NOD mice, PI-88 treatment significantly increased the proportion of pancreatic islets that were intact, significantly reduced the proportion of islets that showed destructive insulitis and better preserved the HS content of the islets (8). This hallmark study has therefore unveiled T1D disease to be largely Hpse-dependent. The extraordinarily high HS content of the beta cells, which is essential for their survival, renders them particularly vulnerable to Hpse-mediated damage. The localization of HS in the islet BM, which by convention acts as a barrier to impede leukocyte infiltration, has also been confirmed in normal human islets (49). Our studies suggest that intracellular HS maintains beta cell survival at least in part, by acting as a “free radical sink,” protecting the beta cells against harmful chemical species generated endogenously. Our findings, which we have subsequently validated in in vitro studies of human islets and beta cells (unpublished data), therefore highlight Hpse inhibitors as a new class of therapeutic that can potentially be used to prevent T1D progression in humans.

Heparanase and Diabetic Complications

The current treatment for T1D is exogenous insulin therapy. While insulin therapy keeps diabetic individuals reasonably healthy, precise control of blood glucose levels invariably fails to be achieved. As a consequence, macrovascular and microvascular diseases develop, resulting in heart disease, nephropathy, retinopathy, and neuropathy. There is compelling evidence that diabetic vascular complications are associated with the accumulation of advanced glycation end products (AGEs) (50). Recent studies have also suggested an important role for Hpse in the development of diabetic nephropathy, a complication pertinent to both Type 1 and Type 2 diabetes. Underpinning this role, involvement of Hpse in proteinuric renal disease has also been established in experimental models of Adriamycin-induced nephropathy and passive Heymann nephritis (22, 51, 52). Diabetic nephropathy is characterized by an increase in the permeability of the glomerular BM (GBM), leading to proteinuria, as well as by tubular and interstitial fibrosis (22, 53). Hyperglycemia has been reported to regulate and, in fact, increase Hpse expression in renal epithelial cells in vitro (54). This finding is consistent with the increased expression of Hpse in renal glomeruli (e.g., glomerular podocytes) in human diabetic nephropathy, with significant increased levels of Hpse in the urine of diabetic patients and with a selective decrease in the expression of GBM HS (5558). Nephropathy resulting from long-term streptozotocin-induced T1D was demonstrated in wildtype mice but not in Hpse-knockout mice (59), supporting a role for Hpse in this condition. The mechanism by which Hpse potentiates diabetic proteinuria may involve altered interactions between glomerular cells and HS-depleted GBM, the release of bioactive molecules from degraded HS, or intracellular signaling in the glomerular cells (22). Hpse is also strongly expressed in renal tubules in diabetic nephropathy and may contribute to tubular fibrosis via effects on FGF-2-signaling (53, 54, 58). An essential role for Hpse in modulating renal tubular morphology was confirmed in diabetic Hpse-knockout mice, which unlike diabetic wildtype controls, were free of histological evidence of tubular fibrosis (59). In vitro studies have implicated albumin overload and AGEs, rather than high glucose, in stimulating Hpse expression via the PI3K/Akt pathway in tubular endothelial cells, and in the subsequent loss of cell surface HS (60). Inhibition of both Hpse activity and the expression of tubular fibrosis markers in vitro by sulodexide furthermore highlights Hpse as a potential therapeutic target for preventing diabetic renal complications (53, 56).

Heparanase may also play a role in diabetic retinopathy. Increased Hpse expression has been demonstrated in high glucose-treated human retinal endothelial cells and in the retinal vascular endothelium of streptozotocin-induced diabetic rats. In vitro, increased levels of active Hpse correlated with enhanced levels of VEGF, a critical angiogenic growth factor required for neovascularization. Upregulation of VEGF in retinal endothelial cells in vitro and in the retina of diabetic rats was inhibited by PI-88, supporting a regulatory role for Hpse, possibly by Src activation (61). At the level of leukocyte adhesion to rat retinal endothelium, an early marker of diabetic retinopathy, Hpse inhibition correlated with the decreased expression of the adhesion molecule ICAM-1 as well as VEGF, properties that implicate Hpse in both the arrest of leukocytes in the retinal vasculature and the associated local vascular dysfunction (62). Parallel studies have further demonstrated a more general role for Hpse in high glucose-induced vascular injury and have provided suggestive evidence for a role for Hpse in the pathogenesis of diabetic atherosclerosis (6365).

Concluding Remarks

The surprising contribution of Hpse to the pathogenesis of T1D in NOD mice, together with the reported involvement of Hpse in the development of vascular complications of diabetes, highlight the potential application of safe and effective Hpse inhibitors for T1D rescue and treatment. Our studies strongly suggest that therapeutic intervention with dual activity Hpse inhibitors/HS mimetics at early stages of the disease could not only prevent the progression of T1D but potentially also fortify the HS content of any remaining beta cells. By preserving the viability and function of residual insulin-producing beta cells, physiological control of glycemia could be maintained without the need for exogenous insulin therapy. Additional benefits of this therapeutic approach would likely extend to preventing the secondary vascular complications of diabetes. Moreover, the studies reviewed here also support the potential treatment of established T1D with Hpse inhibitors to arrest the progression of diabetic vascular diseases, including nephropathy and retinopathy.

Conflict of Interest Statement

Each author is a shareholder in the start-up biotechnology company Beta Therapeutics Pty. Ltd., established to evaluate the therapeutic efficacy of dual activity HS mimetics/heparanase inhibitors in preventing the progression of T1D in humans.

Acknowledgments

This work was supported by a National Health and Medical Research Council of Australia (NHMRC)/Juvenile Diabetes Research Foundation (JDRF) Special Program Grant in Type 1 Diabetes (#418138), a NHMRC Project Grant (#1043284), and a research grant from the Roche Organ Transplantation Research Foundation (ROTRF)/JDRF (#477554991). We thank Anne Prins for histology, Sarah Popp for immunohistochemistry and Russell Kightley Media for graphic design work.

References

1. Hulett MD, Freeman C, Hamdorf BJ, Baker RT, Harris MJ, Parish CR. Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis. Nat Med (1999) 5(7):803–9. doi:10.1038/10525

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

2. Vlodavsky I, Friedmann Y, Elkin M, Aingorn H, Atzmon R, Ishai-Michaeli R, et al. Mammalian heparanase: gene cloning, expression and function in tumor progression and metastasis. Nat Med (1999) 5(7):793–802. doi:10.1038/10518

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

3. Kussie PH, Hulmes JD, Ludwig DL, Patel S, Navarro EC, Seddon AP, et al. Cloning and functional expression of a human heparanase gene. Biochem Biophys Res Commun (1999) 261(1):183–7. doi:10.1006/bbrc.1999.0962

CrossRef Full Text

4. Abboud-Jarrous G, Rangini-Guetta Z, Aingorn H, Atzmon R, Elgavish S, Peretz T, et al. Site-directed mutagenesis, proteolytic cleavage, and activation of human proheparanase. J Biol Chem (2005) 280(14):13568–75. doi:10.1074/jbc.M413370200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

5. Fairbanks MB, Mildner AM, Leone JW, Cavey GS, Mathews WR, Drong RF, et al. Processing of the human heparanase precursor and evidence that the active enzyme is a heterodimer. J Biol Chem (1999) 274(42):29587–90. doi:10.1074/jbc.274.42.29587

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

6. Ilan N, Elkin M, Vlodavsky I. Regulation, function and clinical significance of heparanase in cancer metastasis and angiogenesis. Int J Biochem Cell Biol (2006) 38(12):2018–39. doi:10.1016/j.biocel.2006.06.004

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

7. Miao HQ, Navarro E, Patel S, Sargent D, Koo H, Wan H, et al. Cloning, expression, and purification of mouse heparanase. Protein Expr Purif (2002) 26(3):425–31. doi:10.1016/S1046-5928(02)00558-2

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

8. Ziolkowski AF, Popp SK, Freeman C, Parish CR, Simeonovic CJ. Heparan sulfate and heparanase play key roles in mouse beta cell survival and autoimmune diabetes. J Clin Invest (2012) 122(1):132–41. doi:10.1172/JCI46177

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

9. Esko JD, Selleck SB. Order out of chaos: assembly of ligand binding sites in heparan sulfate. Annu Rev Biochem (2002) 71:435–71. doi:10.1146/annurev.biochem.71.110601.135458

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

10. Kreuger J, Kjellen L. Heparan sulfate biosynthesis: regulation and variability. J Histochem Cytochem (2012) 60(12):898–907. doi:10.1369/0022155412464972

CrossRef Full Text

11. Multhaupt HA, Couchman JR. Heparan sulfate biosynthesis: methods for investigation of the heparanosome. J Histochem Cytochem (2012) 60(12):908–15. doi:10.1369/0022155412460056

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

12. Parish CR. The role of heparan sulphate in inflammation. Nat Rev Immunol (2006) 6(9):633–43. doi:10.1038/nri1918

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

13. Sarrazin S, Lamanna WC, Esko JD. Heparan sulfate proteoglycans. Cold Spring Harb Perspect Biol (2011) 3:a004952. doi:10.1101/cshperspect.a004952

CrossRef Full Text

14. Chen LG, Sanderson RD. Heparanase regulates levels of syndecan-1 in the nucleus. PLoS One (2009) 4(3):e4947. doi:10.1371/journal.pone.0004947

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

15. Richardson TP, Trinkaus-Randall V, Nugent MA. Regulation of heparan sulfate proteoglycan nuclear localization by fibronectin. J Cell Sci (2001) 114(Pt 9):1613–23.

Pubmed Abstract | Pubmed Full Text

16. Vreys V, David G. Mammalian heparanase: what is the message? J Cell Mol Med (2007) 11(3):427–52. doi:10.1111/j.1582-4934.2007.00039.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

17. Abboud-Jarrous G, Atzmon R, Peretz T, Palermo C, Gadea BB, Joyce JA, et al. Cathepsin L is responsible for processing and activation of proheparanase through multiple cleavages of a linker segment. J Biol Chem (2008) 283(26):18167–76. doi:10.1074/jbc.M801327200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

18. Gingis-Velitski S, Zetser A, Kaplan V, Ben-Zaken O, Cohen E, Levy-Adam F, et al. Heparanase uptake is mediated by cell membrane heparan sulfate proteoglycans. J Biol Chem (2004) 279(42):44084–92. doi:10.1074/jbc.M402131200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

19. Goldshmidt O, Nadav L, Aingorn H, Irit C, Feinstein N, Ilan N, et al. Human heparanase is localized within lysosomes in a stable form. Exp Cell Res (2002) 281(1):50–62. doi:10.1006/excr.2002.5651

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

20. Nadav L, Eldor A, Yacoby-Zeevi O, Zamir E, Pecker I, Ilan N, et al. Activation, processing and trafficking of extracellular heparanase by primary human fibroblasts. J Cell Sci (2002) 115(Pt 10):2179–87.

Pubmed Abstract | Pubmed Full Text

21. Zetser A, Levy-Adam F, Kaplan V, Gingis-Velitski S, Bashenko Y, Schubert S, et al. Processing and activation of latent heparanase occurs in lysosomes. J Cell Sci (2004) 117(Pt 11):2249–58. doi:10.1242/jcs.01068

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

22. van den Hoven MJ, Rops AL, Vlodavsky I, Levidiotis V, Berden JH, van der Vlag J. Heparanase in glomerular diseases. Kidney Int (2007) 72(5):543–8. doi:10.1038/sj.ki.5002337

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

23. Peterson S, Liu J. Deciphering mode of action of heparanase using structurally defined oligosaccharides. J Biol Chem (2012) 287(41):34836–43. doi:10.1074/jbc.M112.390161

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

24. Lerner I, Hermano E, Zcharia E, Rodkin D, Bulvik R, Doviner V, et al. Heparanase powers a chronic inflammatory circuit that promotes colitis-associated tumorigenesis in mice. J Clin Invest (2011) 121(5):1709–21. doi:10.1172/JCI43792

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

25. Wood RJ, Hulett MD. Cell surface-expressed cation-independent mannose 6-phosphate receptor (CD222) binds enzymatically active heparanase independently of mannose 6-phosphate to promote extracellular matrix degradation. J Biol Chem (2008) 283(7):4165–76. doi:10.1074/jbc.M708723200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

26. Chen G, Wang D, Vikramadithyan R, Yagyu H, Saxena U, Pillarisetti S, et al. Inflammatory cytokines and fatty acids regulate endothelial cell heparanase expression. Biochemistry (Mosc) (2004) 43(17):4971–7. doi:10.1021/bi0356552

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

27. Edovitsky E, Lerner I, Zcharia E, Peretz T, Vlodavsky I, Elkin M. Role of endothelial heparanase in delayed-type hypersensitivity. Blood (2006) 107(9):3609–16. doi:10.1182/blood-2005-08-3301

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

28. Shafat I, Vlodavsky I, Ilan N. Characterization of mechanisms involved in secretion of active heparanase. J Biol Chem (2006) 281(33):23804–11. doi:10.1074/jbc.M602762200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

29. Wang F, Wang Y, Zhang DH, Puthanveetil P, Johnson JD, Rodrigues B. Fatty acid-induced nuclear translocation of heparanase uncouples glucose metabolism in endothelial cells. Arterioscler Thromb Vasc Biol (2012) 32(2):406–U568. doi:10.1161/Atvbaha.111.240770

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

30. Freeman C, Parish CR. Human platelet heparanase: purification, characterization and catalytic activity. Biochem J (1998) 330(Pt 3):1341–50.

Pubmed Abstract | Pubmed Full Text

31. Gilat D, Hershkoviz R, Goldkorn I, Cahalon L, Korner G, Vlodavsky I, et al. Molecular behavior adapts to context: heparanase functions as an extracellular matrix-degrading enzyme or as a T cell adhesion molecule, depending on the local pH. J Exp Med (1995) 181(5):1929–34. doi:10.1084/jem.181.5.1929

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

32. Goldshmidt O, Zcharia E, Cohen M, Aingorn H, Cohen I, Nadav L, et al. Heparanase mediates cell adhesion independent of its enzymatic activity. FASEB J (2003) 17(9):1015–25. doi:10.1096/fj.02-0773com

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

33. Sotnikov I, Hershkoviz R, Grabovsky V, Ilan N, Cahalon L, Vlodavsky I, et al. Enzymatically quiescent heparanase augments T cell interactions with VCAM-1 and extracellular matrix components under versatile dynamic contexts. J Immunol (2004) 172(9):5185–93.

Pubmed Abstract | Pubmed Full Text

34. Zetser A, Bashenko Y, Edovitsky E, Levy-Adam F, Vlodavsky I, Ilan N. Heparanase induces vascular endothelial growth factor expression: correlation with p38 phosphorylation levels and Src activation. Cancer Res (2006) 66(3):1455–63. doi:10.1158/0008-5472.CAN-05-1811

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

35. Ramani VC, Yang Y, Ren Y, Nan L, Sanderson RD. Heparanase plays a dual role in driving hepatocyte growth factor (HGF) signaling by enhancing HGF expression and activity. J Biol Chem (2011) 286(8):6490–9. doi:10.1074/jbc.M110.183277

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

36. Buczek-Thomas JA, Hsia E, Rich CB, Foster JA, Nugent MA. Inhibition of histone acetyltransferase by glycosaminoglycans. J Cell Biochem (2008) 105(1):108–20. doi:10.1002/Jcb.21803

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

37. He YQ, Sutcliffe EL, Bunting KL, Li J, Goodall KJ, Poon IK, et al. The endoglycosidase heparanase enters the nucleus of T lymphocytes and modulates H3 methylation at actively transcribed genes via the interplay with key chromatin modifying enzymes. Transcription (2012) 3(3):130–45. doi:10.4161/trns.19998

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

38. Weber KS, von Hundelshausen P, Clark-Lewis I, Weber PC, Weber C. Differential immobilization and hierarchical involvement of chemokines in monocyte arrest and transmigration on inflamed endothelium in shear flow. Eur J Immunol (1999) 29(2):700–12. doi:10.1002/(SICI)1521-4141(199902)29:02<700::AID-IMMU700>3.0.CO;2-1

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

39. Benhamron S, Nechushtan H, Verbovetski I, Krispin A, Abboud-Jarrous G, Zcharia E, et al. Translocation of active heparanase to cell surface regulates degradation of extracellular matrix heparan sulfate upon transmigration of mature monocyte-derived dendritic cells. J Immunol (2006) 176(11):6417–24.

Pubmed Abstract | Pubmed Full Text

40. Vlodavsky I, Eldor A, Haimovitz-Friedman A, Matzner Y, Ishai-Michaeli R, Lider O, et al. Expression of heparanase by platelets and circulating cells of the immune system: possible involvement in diapedesis and extravasation. Invasion Metastasis (1992) 12(2):112–27.

Pubmed Abstract | Pubmed Full Text

41. Hershkoviz R, Mor F, Miao HQ, Vlodavsky I, Lider O. Differential effects of polysulfated polysaccharide on experimental encephalomyelitis, proliferation of autoimmune T cells, and inhibition of heparanase activity. J Autoimmun (1995) 8(5):741–50. doi:10.1006/jaut.1995.0055

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

42. Parish CR, Hindmarsh EJ, Bartlett MR, Staykova MA, Cowden WB, Willenborg DO. Treatment of central nervous system inflammation with inhibitors of basement membrane degradation. Immunol Cell Biol (1998) 76(1):104–13. doi:10.1046/J.1440-1711.1998.00722.X

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

43. Li RW, Freeman C, Yu D, Hindmarsh EJ, Tymms KE, Parish CR, et al. Dramatic regulation of heparanase activity and angiogenesis gene expression in synovium from patients with rheumatoid arthritis. Arthritis Rheum (2008) 58(6):1590–600. doi:10.1002/Art.23489

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

44. Waterman M, Ben-Izhak O, Eliakim R, Groisman G, Vlodavsky I, Ilan N. Heparanase upregulation by colonic epithelium in inflammatory bowel disease. Mod Pathol (2007) 20(1):8–14. doi:10.1038/Modpathol.3800710

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

45. Solomon M, Sarvetnick N. The pathogenesis of diabetes in the NOD mouse. Adv Immunol (2004) 84:239–64. doi:10.1016/s0065-2776(04)84007-0

CrossRef Full Text

46. D’Alise AM, Auyeung V, Feuerer M, Nishio J, Fontenot J, Benoist C, et al. The defect in T-cell regulation in NOD mice is an effect on the T-cell effectors. Proc Natl Acad Sci U S A (2008) 105(50):19857–62. doi:10.1073/pnas.0810713105

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

47. Irving-Rodgers HF, Ziolkowski AF, Parish CR, Sado Y, Ninomiya Y, Simeonovic CJ, et al. Molecular composition of the peri-islet basement membrane in NOD mice: a barrier against destructive insulitis. Diabetologia (2008) 51(9):1680–8. doi:10.1007/S00125-008-1085-X

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

48. Tiedge M, Lortz S, Drinkgern J, Lenzen S. Relation between antioxidant enzyme gene expression and antioxidative defense status of insulin-producing cells. Diabetes (1997) 46(11):1733–42. doi:10.2337/diab.46.11.1733

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

49. Korpos E, Kadri N, Kappelhoff R, Wegner J, Overall CM, Weber E, et al. The peri-islet basement membrane, a barrier to infiltrating leukocytes in type 1 diabetes in mouse and human. Diabetes (2013) 62(2):531–42. doi:10.2337/db12-0432

CrossRef Full Text

50. Forbes JM, Cooper ME. Mechanisms of diabetic complications. Physiol Rev (2013) 93(1):137–88. doi:10.1152/Physrev.00045.2011

CrossRef Full Text

51. Kramer A, van den Hoven M, Rops A, Wijnhoven T, van den Heuvel L, Lensen J, et al. Induction of glomerular heparanase expression in rats with adriamycin nephropathy is regulated by reactive oxygen species and the renin-angiotensin system. J Am Soc Nephrol (2006) 17(9):2513–20. doi:10.1681/Asn.2006020184

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

52. Levidiotis V, Freeman C, Tikellis C, Cooper ME, Power DA. Heparanase is involved in the pathogenesis of proteinuria as a result of glomerulonephritis. J Am Soc Nephrol (2004) 15(1):68–78. doi:10.1097/01.Asn.0000103229.25389.40

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

53. Masola V, Onisto M, Zaza G, Lupo A, Gambaro G. A new mechanism of action of sulodexide in diabetic nephropathy: inhibits heparanase-1 and prevents FGF-2-induced renal epithelial-mesenchymal transition. J Transl Med (2012) 10:213. doi:10.1186/1479-5876-10-213

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

54. Maxhimer JB, Somenek M, Rao G, Pesce CE, Baldwin D, Gattuso P, et al. Heparanase-1 gene expression and regulation by high glucose in renal epithelial cells – a potential role in the pathogenesis of proteinuria in diabetic patients. Diabetes (2005) 54(7):2172–8. doi:10.2337/Diabetes.54.7.2172

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

55. Rops AL, van den Hoven MJ, Veldman BA, Salemink S, Vervoort G, Elving LD, et al. Urinary heparanase activity in patients with Type 1 and Type 2 diabetes. Nephrol Dial Transplant (2012) 27(7):2853–61. doi:10.1093/ndt/gfr732

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

56. Shafat I, Ilan N, Zoabi S, Vlodavsky I, Nakhoul F. Heparanase levels are elevated in the urine and plasma of type 2 diabetes patients and associate with blood glucose levels. PLoS One (2011) 6(2):e17312. doi:10.1371/journal.pone.0017312

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

57. van den Hoven MJ, Rops AL, Bakker MA, Aten J, Rutjes N, Roestenberg P, et al. Increased expression of heparanase in overt diabetic nephropathy. Kidney Int (2006) 70(12):2100–8. doi:10.1038/sj.ki.5001985

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

58. Wijnhoven TJ, van den Hoven MJ, Ding H, van Kuppevelt TH, van der Vlag J, Berden JH, et al. Heparanase induces a differential loss of heparan sulphate domains in overt diabetic nephropathy. Diabetologia (2008) 51(2):372–82. doi:10.1007/s00125-007-0879-6

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

59. Gil N, Goldberg R, Neuman T, Garsen M, Zcharia E, Rubinstein AM, et al. Heparanase is essential for the development of diabetic nephropathy in mice. Diabetes (2012) 61(1):208–16. doi:10.2337/db11-1024

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

60. Masola V, Gambaro G, Tibaldi E, Onisto M, Abaterusso C, Lupo A. Regulation of heparanase by albumin and advanced glycation end products in proximal tubular cells. Biochim Biophys Acta (2011) 1813(8):1475–82. doi:10.1016/J.Bbamcr.2011.05.004

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

61. Ma PP, Luo Y, Zhu XB, Li T, Hu J, Tang SB. Retinal heparanase expression in streptozotocin-induced diabetic rats. Can J Ophthalmol (2010) 45(1):46–51. doi:10.3129/I09-200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

62. Ma PP, Luo Y, Zhu XB, Ma HJ, Hu J, Tang SB. Phosphomannopentaose sulfate (PI-88) inhibits retinal leukostasis in diabetic rat. Biochem Biophys Res Commun (2009) 380(2):402–6. doi:10.1016/J.Bbrc.2009.01.092

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

63. Han JY, Mandal AK, Hiebert LM. Endothelial cell injury by high glucose and heparanase is prevented by insulin, heparin and basic fibroblast growth factor. Cardiovasc Diabetol (2005) 4: doi:10.1186/1475-2840-4-12

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

64. Han JY, Woytowich AE, Mandal AK, Hiebert LM. Heparanase upregulation in high glucose-treated endothelial cells is prevented by insulin and heparin. Exp Biol Med (2007) 232(7):927–34.

Pubmed Abstract | Pubmed Full Text

65. Rao G, Ding HG, Huang W, Le D, Maxhimer JB, Oosterhof A, et al. Reactive oxygen species mediate high glucose-induced heparanase-1 production and heparan sulphate proteoglycan degradation in human and rat endothelial cells: a potential role in the pathogenesis of atherosclerosis. Diabetologia (2011) 54(6):1527–38. doi:10.1007/S00125-011-2110-Z

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Keywords: heparanase, heparan sulfate, islet, diabetes, inflammation, vascular complications

Citation: Simeonovic CJ, Ziolkowski AF, Wu Z, Choong FJ, Freeman C and Parish CR (2013) Heparanase and autoimmune diabetes. Front. Immunol. 4:471. doi: 10.3389/fimmu.2013.00471

Received: 11 October 2013; Accepted: 05 December 2013;
Published online: 26 December 2013.

Edited by:

Deirdre Coombe, Curtin University, Australia

Reviewed by:

Fulvio D’Acquisto, Queen Mary University of London, UK
Michael Elkin, Hadassah Hebrew University Hospital, Israel

Copyright: © 2013 Simeonovic, Ziolkowski, Wu, Choong, Freeman and Parish. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Charmaine J. Simeonovic, Diabetes/Transplantation Immunobiology Laboratory, Department of Immunology, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT 2601, Australia e-mail: charmaine.simeonovic@anu.edu.au

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