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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2018.00293

Zearalenone exposure enhanced the expression of tumorigenesis genes in donkey granulosa cells via the PTEN/PI3K/AKT signaling pathway

Guo-Liang Zhang1, 2, Jun-Lin Song3, Chuan-Liang Ji2, Yu-Long Feng2, Jie Yu2, Charles Martin Nyachoti4 and  Gong-She Yang1*
  • 1College of Animal Science and Technology, Northwest A&F University, China
  • 2National Engineering Research Center for Gelatin-based Traditional Chinese Medicine, China
  • 3Qingdao Agricultural University, China
  • 4Department of Soil Science, University of Manitoba, Canada

Zearalenone (ZEA) is a natural contaminant present in food and feed products. It cause reproductive disorders in domestic animals. In China, donkeys have high economic value and are at a high risk of exposure to ZEA. However, no information is available on the effects of ZEA-induced toxicity in donkeys. We used RNA-seq analysis to investigate the biological effects of ZEA on donkey granulosa cells (GCs). ZEA was administered at the concentration of 10 or 30 μM to GCs after 72 h of in vitro culture. The expression of tumorigenesis-associated genes in donkey GCs was drastically altered by exposure to 10 μM ZEA. Exposure to 10 or 30 μM ZEA considerably decreased the mRNA expression levels of PTEN, TGFβ, ATM, and CDK2 genes in GCs. ZEA exposure increased the expression of PI3K and AKT genes. Immunofluorescence, RT-qPCR, and Western blot analyses verified the gene expression patterns of ZEA-exposed GCs. Our results collectively demonstrate that ZEA exerts deleterious effects on the induction of ovarian-cancer-related genes via the PTEN/PI3K/AKT signaling pathway in donkey GCs in vitro.

Keywords: Donkey, Granulosa Cells, tumorigenesis, Gene Expression, RNA-Seq

Received: 08 Apr 2018; Accepted: 13 Jul 2018.

Edited by:

Pierre R. Bushel, National Institute of Environmental Health Sciences (NIEHS), United States

Reviewed by:

ClarLynda Williams-DeVane, North Carolina Central University, United States
Xu Wang, Auburn University, United States  

Copyright: © 2018 Zhang, Song, Ji, Feng, Yu, Nyachoti and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Gong-She Yang, College of Animal Science and Technology, Northwest A&F University, Xianyang, China,