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Review ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2019.01153

Coordinated Regulation of Rsd and RMF for Simultaneous Hibernation of Transcription Apparatus and Translation Machinery in Stationary-phase Escherichia coli

 Hideji Yoshida1*, Akira Wada2,  Tomohiro Shimada3, Yasushi Maki1 and  Akira ISHIHAMA4*
  • 1Osaka Medical College, Japan
  • 2Yoshida Biological Laboratory, Japan
  • 3Faculty of Agriculture, Meiji University, Japan
  • 4Research Center for Micro-Nano Technology, Hosei University, Japan

Transcription and translation in growing phase of Escherichia coli, the best-studied model prokaryote, are coupled and regulated in coordinate fashion. Accordingly, the growth rate-dependent control of the synthesis of RNA polymerase (RNAP) core enzyme (the core component of transcription apparatus) and ribosomes (the core component of translation machinery) is tightly coordinated to keep the relative level of transcription apparatus and translation machinery constant for effective and efficient utilization of resources and energy. Upon entry into the stationary phase, transcription apparatus is modulated by replacing RNAP core-associated sigma (promoter recognition subunit) from growth-related RpoD to stationary phase-specific RpoS. The anti-sigma factor Rsd participates for the efficient replacement of sigma, and the unused RpoD is stored silent as Rsd-RpoD complex. On the other hand, functional 70S ribosome is transformed into inactive 100S dimer by two regulators, RMF (ribosome modulation factor) and HPF (hibernation promoting factor). In this review article, we overview how we found these factors and what we know about the molecular mechanisms for silencing transcription apparatus and translation machinery by these factors. In addition, we provide our recent findings of PS-TF (promoter-specific transcription factor) screening of the transcription factors involved in regulation of the rsd and rmf genes. Results altogether indicate the coordinated regulation of Rsd and RMF for simultaneous hibernation of transcription apparatus and translation machinery.

Keywords: RNA polymerase sigma factor, anti-sigma factor (Rsd), ribosome; ribosome modulation factor (RMF), Hibernation, Stationary phase, Escherichia coli K-12

Received: 20 Jul 2019; Accepted: 22 Oct 2019.

Copyright: © 2019 Yoshida, Wada, Shimada, Maki and ISHIHAMA. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Hideji Yoshida, Osaka Medical College, Takatsuki, Japan, yhide@osaka-med.ac.jp
Prof. Akira ISHIHAMA, Research Center for Micro-Nano Technology, Hosei University, Tokyo, 184-0003, Japan, aishiham@hosei.ac.jp