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Front. Neural Circuits | doi: 10.3389/fncir.2019.00021

rSK1 in rat neurons: a controller of membrane rSK2?

  • 1University of Queensland, Australia
  • 2Queensland Brain Institute, University of Queensland, Australia

In mammalian neurons, small conductance calcium activated potassium channels (SK channels) are activated by calcium influx and contribute to the afterhyperpolarisation that follows action potentials. Three types of SK channel, SK1, SK2 and SK3 are recognised and encoded by separate genes that are widely expressed in overlapping distributions in the mammalian brain. Expression of the rat genes, rSK2 and rSK3 generates functional ion channels that traffic to the membrane as homomeric and heteromeric complexes. However, rSK1 is not trafficked to the plasma membrane, appears not to form functional channels, and the role of rSK1 in neurons is not clear. Here, we show that rSK1 co-assembles with rSK2. rSK1 is not trafficked to the membrane but is retained in a cytoplasmic compartment. When rSK2 is also present, heteromeric rSK1-rSK2 channels are also retained in the cytosolic compartment, reducing the total SK channel content on the plasma membrane. Thus, rSK1 appears to act as chaperone for rSK2 channels and expression of rSK1 may control the level of functional SK current in rat neurons.

Keywords: Spike frequency adaptation, potassium channel, afterhyperpolarization, excitability, calcium activated K+ channels (KCa1-KCa5

Received: 09 Jul 2018; Accepted: 11 Mar 2019.

Edited by:

George Augustine, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore

Reviewed by:

Robert Brenner, The University of Texas Health Science Center at San Antonio, United States
Paul F. Kramer, National Institute of Neurological Disorders and Stroke (NINDS), United States  

Copyright: © 2019 Sah. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Pankaj Sah, University of Queensland, Brisbane, Australia, pankaj.sah@uq.edu.au