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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Neural Circuits | doi: 10.3389/fncir.2019.00074

Visualization of brain activity in a neuropathic pain model using quantitative activity-dependent manganese magnetic resonance imaging

 Chihiro Inami1, Hiroki Tanihira2,  Satomi Kikuta3, Osamu Ogasawara1, Kazuya Sobue1,  Kazuhiko Kume1,  Makoto Osanai2 and  Masahiro Ohsawa1*
  • 1Nagoya City University, Japan
  • 2Tohoku University, Japan
  • 3Kyoto University, Japan

Human brain imaging studies have revealed several regions that are activated in patients with chronic pain. In rodent brains, functional changes due to chronic pain have not been fully elucidated, as brain imaging techniques such as functional magnetic resonance imaging and positron emission tomography require the use of anesthesia to suppress movement. Consequently, conclusions derived from existing imaging studies in rodents may not accurately reflect brain activity under awake conditions. In this study, we used quantitative activation-induced manganese-enhanced magnetic resonance imaging to directly capture the previous brain activity of awake mice. We also observed and quantified the brain activity of the spared nerve injury (SNI) neuropathic pain model during awake conditions. SNI-operated mice exhibited a robust decrease of mechanical nociceptive threshold 14 days after nerve injury. Imaging on SNI-operated mice revealed increased neural activity in the limbic system and secondary somatosensory, sensory-motor, piriform, and insular cortex. We present the first study demonstrating a direct measurement of awake neural activity in a neuropathic pain mouse model.

Keywords: neuropathic pain, MRI, Manganase, Somatosensory abnormality, emotion

Received: 12 Nov 2018; Accepted: 31 Oct 2019.

Copyright: © 2019 Inami, Tanihira, Kikuta, Ogasawara, Sobue, Kume, Osanai and Ohsawa. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Masahiro Ohsawa, Nagoya City University, Nagoya, Japan, ohsawa@phar.nagoya-cu.ac.jp