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Front. Physiol. | doi: 10.3389/fphys.2018.00118

Behavioral, Morphological and Gene Expression Changes Induced by 60Co-γ Ray Irradiation in Bactrocera tau (Walker)

 Jun Cai1, Hongxia Yang2, Song Shi1,  Guohua Zhong1 and  Xin Yi1*
  • 1South China Agricultural University, China
  • 2Guangdong Entry-Exit Inspection and Quarantine Technology Center, China

The sterile insect technique (SIT) may reduce pest populations by allowing sufficient amount of irradiation-induced sterile males to mate with wild females whilst maintaining mating ability comparable to wild males. Although the SIT methods are well understood, the optimal sterilizing dose and processing development stage for application vary among species. To ensure effective pest control programs, effects of irradiation on physiology, behavior, and gene function in the target species should be defined, however, little is known about irradiation effects in Bactrocera tau. Here, the effects of irradiation on rates of fecundity, egg hatch, eclosion, mating competitiveness, flight capability, morphology of reproductive organs, and yolk protein (YP) gene expression were studied. The results showed that rates of female fecundity and egg hatch decreased significantly (51 ±19 to 0.06 ±0.06 and 98.90 ±1.01 to 0, respectively) when pupae were treated with > 150 Gy irradiation. Flight capability and mating competitiveness were not significantly influenced at doses < 250 Gy. Ovaries and fallopian tubes became smaller after irradiation, but there was no change in testes size. Finally, we found that expression of the YP gene was up-regulated by irradiation at 30 and 45 days post-emergence, but the mechanisms were unclear. Our study provides information on the determination of the optimal irradiation sterilizing dose in B. tau, and the effects of irradiation on physiology, morphology and gene expression that will facilitate an understanding of sub-lethal impacts of the SIT and expand its use to the control of other species.

Keywords: Bactrocera tau, sterile insect technique, Optimal dose, yolk polypeptides, Morphological changes

Received: 07 Nov 2017; Accepted: 05 Feb 2018.

Edited by:

Bin Tang, Hangzhou Normal University, China

Reviewed by:

Xiaoling Tan, Chinese Academy of Agricultural Sciences, China
Muhammad Rizwan Ul Haq, King Faisal University, Saudi Arabia
Genevieve Lanouette, Université de Montréal, Canada  

Copyright: © 2018 Cai, Yang, Shi, Zhong and Yi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Xin Yi, South China Agricultural University, Guangzhou, China, yixin423@126.com