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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Physiol. | doi: 10.3389/fphys.2019.01223

Identification and Expression Analyses of Olfactory Gene Families in the Rice Grasshopper, Oxya chinensis, from Antennal Transcriptomes

  • 1Guang Zhou City Key Laboratory of Subtropical Fruit Tree Outbreak Control, Zhongkai University of Agriculture and Engineering, China

The rice grasshopper Oxya chinensis is an important agricultural pest of rice and other gramineous plants. Chemosensory genes are crucial factors in direct interactions with odorants in the olfactory process. Here we identified genes encoding 18 odorant binding proteins (OBPs), 13 chemosensory proteins (CSPs), 94 odorant receptors (ORs), 12 ionotropic receptors (IRs), and two sensory neuron membrane proteins (SNMPs) from O. chinensis using an transcriptomic approach. Semi-quantitative RT-PCR assays revealed that six OBP-encoding genes (OchiOBP4, 5, 8, 9, 10 and 14), one CSP gene (OchiOBP10) and two IR genes (OchiIR28 and 29) were exclusively expressed in antennae, suggesting their roles in olfaction. Real-time quantitative PCR analyses revealed that genes expressed exclusively or predominantly in antennae also displayed significant differences in expression levels between males and females. Among the differentially expressed genes, 17 OR-encoding genes, one CSP- and one SNMP-gene showed female-biased expression, suggesting that they may be involved in some female-specific behaviors such as seeking oviposition site; whereas the three remaining OR-encoding genes showed male-biased expression, indicating their possible roles in sensing female sex pheromones. Our results laid a solid foundation for future studies to reveal olfactory mechanisms as well as designing strategies for controlling this rice pest.

Keywords: Antennal transcriptome, olfactory gene, IDENTIFICATION, expression analysis, Oxya chinensis

Received: 27 Jul 2019; Accepted: 09 Sep 2019.

Copyright: © 2019 Cui:, Kang, Wu and Lin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Mx. Zhonzhen Wu, Zhongkai University of Agriculture and Engineering, Guang Zhou City Key Laboratory of Subtropical Fruit Tree Outbreak Control, Guangzhou, China, zhongzhen_wu@163.com
Prof. Jintian Lin, Zhongkai University of Agriculture and Engineering, Guang Zhou City Key Laboratory of Subtropical Fruit Tree Outbreak Control, Guangzhou, China, linjtian@163.com