Abstract
The superior paraolivary nucleus (SPON), a prominent GABAergic center of the mammalian auditory brainstem, projects to the ipsilateral inferior colliculus (IC) and sends axons through the commissure of the IC (CoIC). Herein we demonstrate that the SPON is reciprocally connected with the recently discovered tectal longitudinal column (TLC). The TLC is a long and narrow structure that spans nearly the entire midbrain tectum longitudinally, immediately above the periaqueductal gray matter (PAG) and very close to the midline. Unilateral injections of biotinylated dextran into the SPON of the rat label abundant terminal fibers in the TLC of both sides, with an ipsilateral predominance. The SPON provides a dense innervation of the entire rostrocaudal extent of the ipsilateral TLC, and a relatively sparser innervation of the caudal and rostral portions of the contralateral TLC. SPON fibers reach the TLC by two routes: as collaterals of axons of the CoIC, and as axons that circumvent the ipsilateral IC before traveling in the deep layers of the superior colliculus (SC). The density of these projections identifies SPON as a significant source of input to the TLC. Other targets of the SPON discovered in this study include the deep layers of the SC and the PAG. The same experiments reveal numerous labeled cell bodies in the TLC, interspersed among the labeled SPON fibers. This observation suggests that the SPON is a significant target of TLC projections. The discovery of novel reciprocal connections between the SPON and the TLC opens unexpected avenues for investigation of sound processing in mammalian brainstem circuits.
Introduction
The tectal longitudinal column (TLC) spans the midbrain tectum longitudinally, immediately above the periaqueductal gray matter (PAG) and very close to the midline. In the rat, the TLC extends approximately 3.5 mm, from the caudal end of the commissure of the inferior colliculus (CoIC) to the rostral end of the commissure of the superior colliculus (CoSC), and occupies what has traditionally been considered the medial-most region of the deep superior colliculus (SC) and the medial-most region of the inferior colliculus (IC). Despite measuring only 250–350 μm in diameter, more than 11,500 neurons are packed within this long and narrow structure (Saldaña et al., 2007).
The caudal and rostral thirds of the TLC are crossed by the CoIC and by the CoSC (Saldaña et al., 2007), respectively, suggesting that these fiber tracts are a preferred pathway of entry for afferent inputs to the TLC. The CoIC contains mostly commissural axons of the IC, as well as axons from other sources or destined for other targets (reviewed by Saldaña and Merchán, ). These include axons from various subcollicular auditory nuclei, namely the sagulum and nuclei of the lateral lemniscus (González-Hernández et al., ; Henkel and Shneiderman ; Hutson et al., ; Bajo et al., ). Recently, it has been demonstrated that the superior paraolivary nucleus (SPON), a prominent cell group of the superior olivary complex (SOC), also projects axons across the CoIC (Saldaña et al., ). Thus, we set forth to investigate whether the SPON, which is likely the caudal-most auditory center sending projections across the CoIC, innervates the TLC. We chose to carry out this investigation in rats because the TLC has been best characterized in this species (Saldaña et al., 2007; Marshall et al., ; Aparicio et al., ); moreover, abundant information is available concerning the structure, connections and physiology of the rat SPON (Kulesza Jr. and Berrebi, ; Saldaña and Berrebi, ; Kulesza Jr. et al., , ; Kadner et al., ; Kadner and Berrebi, ; Saldaña et al., ).
Materials and Methods
Thirteen female albino rats (body weight 190–210 g) with clean ear canals and no sign of middle ear infection were used in this study. These specimens were also used for the study of SPON projections to the IC described in a previous article (Saldaña et al., ). All animals were cared for and used in compliance with European Union regulations concerning the use of animals in biomedical research, and the experimental procedures were approved and supervised by the Animal Care and Use Committee of the University of Salamanca. For surgical procedures, including the transcardial perfusion of fixatives, all animals were deeply anesthetized with a mixture of ketamine HCl (80 mg/kg body weight) and xylazine (6 mg/kg body weight) administered intramuscularly. Animal suffering was minimized by monitoring the depth of anesthesia often, carefully attending to physiological cues such as rate and depth of respiration and reflex activity. Supplemental doses of anesthetics were given as needed to maintain deep anesthesia throughout all procedures.
Glass micropipettes loaded with the neuroanatomical tracer biotinylated dextran amine (BDA, 10,000 MW, Molecular Probes, Eugene, OR, USA; 10% in 0.1 M sodium phosphate buffer, pH 7.4) were lowered into the SPON of deeply anesthetized rats using stereotaxic coordinates (Paxinos and Watson, ). To avoid damage to the prominent transverse sinus, the pipettes were lowered into the brain via a dorsocaudal to ventrorostral approach, so that their trajectory formed a 16° angle with the coronal plane. The tracer was delivered by iontophoresis using a pulsed 5 μA DC positive current (7 s on/7 s off) for 5–15 min. The current was then stopped and the pipette left in place for an additional 15–20 min prior to withdrawal in order to minimize leakage of the tracer along the injection tract.
Following 7–10 days survival, the rats were anesthetized deeply and their brains fixed by transcardial perfusion of buffered 4% formaldehyde (prepared from freshly depolymerized paraformaldehyde) and 0.1% glutaraldehyde. After cryoprotection in 30% sucrose in phosphate buffer, the brains were cut coronally on a freezing microtome at a thickness of 40 μm. To visualize the tracer, the sections were first processed by the avidin–biotin–peroxidase complex procedure following the manufacturer's specifications (ABC, Vectastain, Vector Labs, Burlingame, CA, USA), and then by standard histochemistry for peroxidase, with or without heavy-metal intensification (Vetter et al., 1993). For cytoarchitectural reference, every fourth section was counterstained with cresyl violet.
Sections were photographed at high resolution using a Zeiss Axioskop 40 microscope equipped with a Zeiss AxioCam MRc 5 digital camera and 2.5× (NA 0.075), 5× (NA 0.15), 10× (NA 0.30), 20× (NA 0.50), and 40× (NA 0.75) plan semi-apochromatic objective lenses. Image brightness and contrast were adjusted with Adobe Photoshop software (Adobe Systems Incorporated, San Jose, CA, USA), and the illustrations were arranged into plates using Canvas software (ACD Systems of America, Inc., Miami, FL, USA).
To generate the drawings of Figure 2, the sections were first photographed at high resolution with the 5× objective lens. At this magnification, several micrographs were needed to photograph every section. These photographs were then arranged and fitted using Adobe Photoshop software to create a large mosaic image of the section. The resulting digital image was imported into Canvas software. To increase the resolution of the final image, a new layer was created over the digital image and each labeled fiber contained within the original micrograph was redrawn digitally using Canvas’ freehand drawing tool. This digital procedure allowed us to subsequently adjust the thickness of the lines. The new digital layer, without the underlying micrograph, was finally saved as a TIFF file.
A similar procedure was used to produce the plots showing the distribution of presumed labeled synaptic boutons in Figures 6 and 7. To convey a clear impression of synaptic bouton density, each plot of Figure 7 was subsequently transferred to a Photoshop document and blurred using a Gaussian filter with a 20-pixel square matrix.
Results
The present results are based on 13 cases with single injections of BDA into the SPON. In 12 cases, the injection sites were localized within the SPON, without encroaching upon neighboring structures (e.g., Figure 1A). In the other case (case 99014), the injection site encroached upon the medial superior olive. The mediolateral diameter of the center of the injection sites ranged from 150 to 380 μm. Figure 1B shows schematically the injection site of seven representative cases.
Figure 1
Projection from the SPON to the TLC
Labeled SPON axons, easily recognizable by their large caliber (>1.2 μm), exit the injection site and travel rostrally, dorsally, and laterally to join the medial-most aspect of the ipsilateral lateral lemniscus, through which they ascend toward the midbrain tectum without entering the nuclei of the lateral lemniscus. Most SPON axons then penetrate into the IC, where they create dense terminal fields in all IC subdivisions. Some SPON axons that reach the dorsal cortex of the IC extend dorsomedially and cross the CoIC, to innervate the dorsal cortex of the contralateral IC. The distribution of labeled fibers in the nuclei of the lateral lemniscus and the IC following injections of BDA into the SPON has been described previously (Saldaña et al., ) and will therefore not be considered further in this account.
Those SPON axons destined to innervate the TLC do so by two different routes. The first route is taken by some of the axons traveling in the CoIC (Figures 2A,B, 3A,B, and 4A). These fibers give off one or more collaterals within the ipsilateral TLC and/or, less frequently, within the contralateral TLC. Occasionally individual labeled SPON fibers give off collaterals to both the ipsilateral and contralateral TLCs (Figure 4A). All of these collaterals are usually thin and tend to run either vertically within the CoIC or rostrally, so that the latter course caudorostrally within the TLC.
Figure 2
Figure 3
Figure 4
The second, more prominent pathway of entry into the TLC is formed by SPON axons that circumvent the ipsilateral IC ventromedially or ventrorostrally to reach the SC. These fibers then run dorsomedially through the deep layers of the SC to reach the ipsilateral TLC, which they enter at different rostrocaudal levels (Figures 2C–F and 3C–F). Upon entering the TLC by this route, SPON axons usually turn caudally and/or rostrally to span the nucleus longitudinally. Most of these axons seem to end within the ipsilateral TLC. However, some of them, particularly those traveling in the CoSC, give off collaterals for the ipsilateral TLC before crossing the midline to innervate the contralateral TLC or even proceeding into the contralateral SC (Figures 2F and 3F). The two routes taken by SPON axons that innervate the TLC are depicted schematically in Figure 5.
Figure 5
It is evident from these observations that the projections from SPON to the ipsilateral and contralateral TLC are asymmetric. SPON fibers innervate densely the entire rostrocaudal length of the ipsilateral TLC, but only the caudal and rostral ends of the contralateral TLC (Figures 2 and 3).
The caudal third of the ipsilateral TLC is innervated preferentially by collaterals of axons that travel in the CoIC (Figures 2A, B, 3A, B, 4A, and 5), whereas the central and the rostral thirds of the nucleus are innervated mostly by axons that run through the deep layers of the SC (Figures 2C–F, 3C–F, 4B, and 5). The density of terminal SPON fibers is maximal in the central rostrocaudal third and decreases toward the caudal and the rostral ends of the TLC (Figures 3 and 6).
Figure 6
The caudal and the rostral portions of the contralateral TLC receive relatively sparse projections from the SPON by way of collaterals of axons that travel in the CoIC (Figures 3A,B) and the CoSC (Figure 3F), respectively. At every rostrocaudal level, the projection from SPON to the contralateral TLC is considerably less dense than the uncrossed projection (Figure 6).
The terminal branches of SPON axons within the TLC are variously oriented, although vertically oriented terminal fibers predominate at caudal levels on both sides (Figures 3A′ and 4A). Despite these varying orientations, the morphology of SPON axons is essentially similar on the two sides and remains rather constant at all rostrocaudal levels. Many terminal branches give off multiple short collaterals with one to three swellings, whereas others end in beaded branches bearing numerous small (<1.5 μm) en passant swellings and a terminal bouton (Figure 4B); all these varicosities and boutons are presumed to represent synaptic specializations.
Topography of projections from the SPON to the TLC
Despite the fact that the projections from the SPON to the IC are clearly topographic and presumably tonotopic (Saldaña et al., ), no topographic organization is readily apparent in the projections from the SPON to the TLC. In all cases, regardless of the location of the injection site within SPON, labeled terminal fibers are widely distributed along the rostrocaudal and mediolateral axes of the TLC (Figure 3). The distribution of terminal fibers along the dorsoventral axis shows variations among different sections in a given case (for instance, in Figures 3B,E the labeled terminal fibers cover the entire dorsoventral diameter of the TLC, whereas in Figures 3C,D terminal fibers are concentrated in the ventral half of the nucleus; see also Figure 6).
Despite the above considerations, a subtle dorsoventral topography is appreciated at caudal levels. The terminal fields of SPON fibers tend to shift their dorsoventral position within the TLC as a function of the position of the injection site along the tonotopic (i.e., mediolateral) axis of the SPON (Figures 7A1–A3,B1–B3). Thus, in cases with tracer deposits in the medial (high characteristic frequency) region of the SPON, labeled terminal fibers are distributed mostly throughout the ventral half of the TLC; conversely, after injections in more lateral (lower characteristic frequency) regions of the SPON, labeled terminal fibers predominate dorsally within the TLC. This slight topographic tendency observed at caudal levels becomes gradually more difficult to discern rostrally (Figures 7A4,A5,B4,B5).
Figure 7
Other targets of SPON projections
Superior paraolivary nucleus neurons send projections to other targets besides the IC and the TLC. For example, even though many SPON axons that circumvent the IC appear to traverse the deep layers of the SC without branching, others give off collaterals that innervate the SC or the PAG (Figures 2, 3, 4B, and 8).
Figure 8
In the SC ipsilateral to the injection site, labeled terminal fibers seem to concentrate in the caudal, ventral, and lateral region of the deep layers, not far from the rostral pole of the IC (Figures 8A,B). A few labeled collaterals ascend radially toward the intermediate and superficial layers, where terminal labeling is very sparse (Figures 8A,C). Very few labeled fibers are observed in layers of the ipsilateral SC more superficial than the intermediate gray layer (Figures 2 and 8).
Terminal labeling in the PAG ipsilateral to the injection site is sparse and widely distributed throughout the lateral, dorsolateral, and dorsomedial columns (Figures 2, 3, and 8A,D). In all cases, a few labeled fibers enter the dorsomedial column of the PAG from the overlying TLC (Figure 4B).
Terminal labeling is observed also in the SC and PAG contralateral to the injection site (Figures 2 and 3). These crossed projections are considerably less dense, however, than their ipsilateral counterparts.
Lastly, we also noted labeled terminal axons in the medial geniculate body ipsilateral to the injection site (Figure 2F). This novel projection from the SPON will be described in detail in a separate account.
Retrogradely labeled neurons in the TLC
In most of our cases, retrogradely labeled cell bodies are found in the ipsilateral TLC (Figure 9), whereas neurons labeled in the contralateral TLC are very scarce (Figure 9E). The number of retrogradely labeled neurons varied from case to case and is apparently not related to the position of the injection site within the SPON. It is noteworthy that such retrogradely labeled neurons are always located within the territory occupied by SPON labeled terminal fibers (Figure 9), suggesting that the SPON and the TLC are reciprocally connected.
Figure 9
Most labeled TLC neurons display a punctate reaction product restricted to the cell body and, sometimes, the proximal dendritic segments; their axons are not labeled (Figures 9A–E). In the rare instances of neurons with diffuse labeling whose axons are labeled for hundreds of micrometers, no axonal collaterals are observed within the TLC (Figure 9F), suggesting that tectobulbar TLC neurons do not possess local recurrent collaterals.
Whereas close appositions between anterogradely labeled axonal swellings of SPON fibers and labeled cell bodies or dendrites in the TLC are occasionally observed, most axonal swellings are seen at a distance from retrogradely labeled neurons (Figure 9). This result suggests that SPON fibers preferentially target TLC neurons that do not innervate the SPON. However, we cannot rule out the possibility that SPON axons also innervate distal-most portions of the dendrites of tectobulbar TLC neurons that remained unlabeled in our experiments.
Discussion
Our experiments have disclosed hitherto unknown projections from the SPON to the TLC of the rat, which constitute a major, novel auditory pathway through the lower brainstem to the midbrain. The SPON targets the entire ipsilateral TLC and, less densely, the caudal and rostral regions of the contralateral TLC. Our study also demonstrates that the SPON and the TLC are reciprocally connected, as the TLC contains abundant neurons that project back to the ipsilateral SPON.
Technical considerations
In all our cases with injections of BDA into the SPON, numerous fibers were labeled in both the ipsilateral and contralateral TLC. This tracer is capable of giving rise to so-called collateral transport, whereby it labels anterogradely the collaterals of the axons through which it is transported retrogradely (i.e., Merchán et al., ; Warr et al., 1997; Doucet and Ryugo, ; Saldaña et al., ). Therefore, in principle, axons labeled with BDA may originate from neurons at the injection site (genuine anterograde transport) or may belong to neurons that innervate or cross the injection site (collateral transport). In our cases, numerous neurons were labeled in the ipsilateral medial nucleus of the trapezoid body (MNTB), and to a lesser extent in the contralateral ventral cochlear nucleus and ipsilateral lateral nucleus of the trapezoid body (see also Saldaña et al., ). It is very unlikely, however, that the axons labeled in the TLC originated from these nuclei for two reasons: first, because labeled neurons are never found in any of these brainstem nuclei following deposits of the sensitive retrograde tracer FluoroGold into the paramedian region of the midbrain tectum that include large portions of the TLC within the injection site; and second, because labeled fibers are never found in the TLC following injections of BDA into the MNTB or the cochlear nuclei (unpublished observations).
In many of our cases, numerous neurons were retrogradely labeled within the TLC, but their axons were not visible (Figure 9). Moreover, the few retrogradely labeled neurons whose axons were labeled did not give off local collaterals (Figure 9F). Furthermore, labeled axons were systematically observed in the contralateral TLC, where retrogradely labeled neurons were extremely scarce. Finally, labeled axons in the TLC were observed in all cases with BDA injection into the SPON, including those that did not exhibit retrogradely labeled TLC neurons. Our interpretation, therefore, is that all BDA-labeled fibers in the TLC originate from the SPON.
The SPON to TLC projection
Our results demonstrate that the SPON sends direct projections to the TLC. These projections are predominantly ipsilateral, and seem to be dense. Given that the number of SPON neurons is relatively modest (approximately 2,500 neurons; Kulesza Jr. et al., ), the density of the projections revealed in this study indicates that the TLC is a main target of the SPON.
It has been known for decades that the main target of SPON projections is the ipsilateral IC (for references, see Saldaña et al., ). Given that virtually all SPON neurons participate in this projection (Saldaña and Berrebi, ), it is highly probable that SPON neurons that innervate the TLC also contribute to the innervation of the IC.
Recently, electrophysiological studies have demonstrated that, unlike neurons in the gerbil SPON, which exhibit considerable heterogeneity in their response properties (Behrend et al., ; Dehmel et al., ), neurons of the rat SPON are remarkably homogeneous. These cells are driven by input from the contralateral ear and strongly suppressed during acoustic stimulation by tonically active glycinergic inhibition that originates in the adjacent MNTB (Banks and Smith, ; Sommer et al., 1993; Smith et al., 1998; Kulesza Jr. et al., ; Kadner et al., ; Kadner and Berrebi, ). When the acoustic stimulus is terminated, SPON neurons are released from this inhibition and fire brief trains of discharges which constitute their characteristic offset response (Kulesza Jr. et al., , ; Kadner and Berrebi, ). Because these offset spikes are triggered by episodes of low stimulus energy, i.e., gaps in ongoing stimuli and the troughs of amplitude modulated tones, Kadner and Berrebi () proposed that the functional relevance of the brainstem MNTB/SPON circuit lies mainly in the encoding of stimulus discontinuities. The precisely timed output of the SPON is then relayed to its synaptic targets in the midbrain, including the TLC.
To understand the contribution of SPON projections to TLC function will require knowledge of the neuroactive substances released by SPON axons and the receptors expressed on the membranes of TLC neurons. Nothing is currently known regarding the latter issue, but several studies indicate that SPON neurons utilize GABA as their neurotransmitter (Mugnaini and Oertel, ; González-Hernández et al., ; Kulesza Jr. and Berrebi, ), so it is reasonable to assume that the projections described in this study are inhibitory. In the only detailed study to record the sound-evoked responses of TLC neurons, Marshall et al. () reported no evidence for tonotopy in the nucleus, relatively broad frequency tuning and very limited sensitivity to amplitude modulation, even within the range of modulation frequencies that trigger highly synchronous action potentials from SPON neurons. Thus, it is difficult at this time to reconcile the physiological response properties of TLC neurons with the precisely timed offset spiking activity of SPON neurons. However, one must keep in mind that TLC neurons integrate their synaptic inputs from multiple sources, only a few of which are identified at this time, namely the auditory cortex (Morest and Oliver, ; Saldaña et al., ), the IC (Morest and Oliver, ; Saldaña and Merchán, , ; Aparicio et al., ) and the SPON, as shown herein.
Projection from the TLC to the spon
Based on the relatively high response thresholds and broad frequency tuning, as well as long first spike latencies, a prevalence for sustained firing patterns and poor phase-locking of TLC neurons, Marshall et al. () concluded that the nucleus functions mainly in the descending modulation of lower auditory centers. This notion is supported by the fact that a large proportion of TLC neurons are immunolabeled by antisera directed against the inhibitory neurotransmitter GABA (our own unpublished immunohistochemical results) or its synthetic enzyme glutamic acid decarboxylase (Mugnaini and Oertel, ).
The present demonstration of TLC neurons retrogradely labeled from SPON injection sites confirms the existence of a descending projection and extends and refines previous studies that reported neurons labeled in the TLC following large deposits of retrograde tracers into the SOC (Faye-Lund, ; Mulders and Robertson, ; Saldaña et al., 2007). Thus, the SPON becomes the first identified target of TLC neurons.
It is also noteworthy that the TLC is the only documented source of descending inputs to the SPON of the rat, as this nucleus seems to be spared by other descending projections to the SOC (but see Thompson and Thompson, 1993; Coomes and Schofield, , for results in the guinea pig). Indeed, descending IC fibers target preferentially the ventral nucleus of the trapezoid body (Faye-Lund, ; Caicedo and Herbert, ; Vetter et al., 1993; Mulders and Robertson, ). Likewise, the projections from the auditory cerebral cortex to the SOC terminate mainly in the ventral nucleus of the trapezoid body and, to a lesser extent, in the lateral superior olive and the so-called dorsal ribbon of the SOC (Feliciano et al., ; Mulders and Robertson, ).
Conclusion
The present tract-tracing investigation revealed two hitherto unknown connections in the rat auditory system: ascending projections from the SPON to the TLC and descending projections from the TLC to the SPON. Both the TLC and SPON have been identified in postmortem human brainstem (Saldaña et al., 2007; Kulesza Jr., ; Kulesza Jr. et al., ), leading us to believe that the circuits discovered in the rat may also be present in humans. In any event, given our current level of understanding of the function of either nucleus, it is not possible at this time to formulate specific testable hypotheses of the biological significance of these reciprocal circuits. Electrophysiological recordings from the TLC while reversibly inactivating the SPON, or vice versa, would provide considerable insight into the impact of these reciprocal projections on auditory processing in the brainstem.
Author Contribution
Enrique Saldaña designed the experiments and supervised the project. Antonio Viñuela and M.-Auxiliadora Aparicio performed the experiments and prepared the illustrations. Antonio Viñuela, M.-Auxiliadora Aparicio, Albert S. Berrebi, and Enrique Saldaña analyzed and interpreted the results. Enrique Saldaña and Albert S. Berrebi wrote the manuscript. Antonio Viñuela and M.-Auxiliadora Aparicio contributed equally to the work.
Statements
Acknowledgments
This work was supported by the Spanish Ministries of Education and Science and Innovation grants PB95-1129, BFI2000/1358, BFU2004-05909, and BFU2008-04197 (to Enrique Saldaña), by the Junta de Castilla y León grants SA15/97, SA097/01, SA007C05, GR221, and Biomedicina 2009 (to Enrique Saldaña) and by the National Institute on Deafness and Other Communication Disorders Grant RO1 DC-002266 (to Albert S. Berrebi). The authors are thankful to Dr. Verónica Fuentes-Santamaría, who performed many of the tracing experiments.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Summary
Keywords
auditory brainstem, commissure of the inferior colliculus, superior colliculus, periaqueductal gray matter, superior olivary complex, biotinylated dextran amine, tract-tracing
Citation
Viñuela A, Aparicio M-A, Berrebi AS and Saldaña E (2011) Connections of the Superior Paraolivary Nucleus of the Rat: II. Reciprocal Connections with the Tectal Longitudinal Column. Front. Neuroanat. 5:1. doi: 10.3389/fnana.2011.00001
Received
23 July 2010
Accepted
05 January 2011
Published
22 February 2011
Volume
5 - 2011
Edited by
Enrico Mugnaini, Northwestern University, USA
Reviewed by
Marina Bentivoglio, University of Verona, Italy; Douglas L. Oliver, University of Connecticut Health Center, USA; Donata Oertel, University of Wisconsin, USA; Joe Adams, Massachusetts Eye and Ear Infirmary, USA
Copyright
© 2011 Viñuela, Aparicio, Berrebi and Saldaña.
This is an open-access article subject to an exclusive license agreement between the authors and Frontiers Media SA, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.
*Correspondence: Enrique Saldaña, Laboratory for the Neurobiology of Hearing, Neuroscience Institute of Castilla y León, University of Salamanca, Salamanca 37007, Spain.e-mail: saldana@usal.es
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.