Abstract
The expression pattern of Sonic Hedgehog (Shh) in the developing hypothalamus changes over time. Shh is initially expressed in the prechordal mesoderm and later in the hypothalamic neuroepithelium—first medially, and then in two off-medial domains. This dynamic expression suggests that Shh might regulate several aspects of hypothalamic development. To gain insight into them, lineage tracing, (conditional) gene inactivation in mouse, in ovo loss- and gain-of-function approaches in chick and analysis of Shh expression regulation have been employed. We will focus on mouse studies and refer to chick and fish when appropriate to clarify. These studies show that Shh-expressing neuroepithelial cells serve as a signaling center for neighboring precursors, and give rise to most of the basal hypothalamus (tuberal and mammillary regions). Shh signaling is initially essential for hypothalamic induction. Later, Shh signaling from the neuroepithelium controls specification of the lateral hypothalamic area and growth-patterning coordination in the basal hypothalamus. To further elucidate the role of Shh in hypothalamic development, it will be essential to understand how Shh regulates the downstream Gli transcription factors.
Expression pattern of Shh in the developing hypothalamus
Already the first studies characterizing mouse embryonic Sonic Hedgehog (Shh) expression showed that it is dynamic in the developing forebrain, a fact that immediately led to speculation as to the function of these domains (Echelard et al., ; Chang et al., ; Shimamura et al., ; Goodrich et al., ; Platt et al., ; Figures 1A–D). Based on these early studies and our own detailed hypothalamus expression analysis (Szabó et al., ; Alvarez-Bolado et al., ), we divide the expression of Shh in the mouse neural tube into distinct patterns (Figures 1A–D). Similar patterns are found in chick (Dale et al., ; Ohyama et al., , ; Placzek and Briscoe, ; Manning et al., ) and zebrafish (Barth and Wilson, ; Mathieu et al., ) embryos. Note that the dates in embryonic days (E) are approximate, and, for some events, differences of up to 1 day can be found between studies. The influence of Shh on the hypothalamus starts with the onset of Shh expression in the underlying head process (Figures 1A,E, early pattern) (Aoto et al., ) (chick HH stage [st] 4). This is accompanied by the almost immediate onset of Gli1 expression in the overlying neural ectoderm, starting at E7.5 (Figure 1E; Hui et al., ). Since Gli1 expression is diagnostic of Shh pathway activation (Goodrich et al., ; Marigo and Tabin, ; Marigo et al., ; Lee et al., ), Gli1 expression indicates that Shh signaling is involved in specifiying this neuroectodermal region. At E8.5, the neuroectodermal cells in the ventral midline start to express Shh (i.e., neuroepithelial Shh makes its appearance) (chick st 7–10; zebrafish 5 somites) while Gli1 expression is downregulated medially (Christ et al., ; Figure 1F). Later, Shh expression is downregulated in the ventral midline of the basal hypothalamus and Shh is expressed in two domains bilaterally to the midline (Figure 1G, late pattern) (chick st 15 and later; zebrafish 22–28 somites). We still lack detailed studies of Gli1 expression in the alar and basal hypothalamus at E9.5–E10.5. The scarce expression data found in the literature (Furimsky and Wallace, ; Aoto et al., ) are imprecise in terms of hypothalamic region as well as plane of section. In chick, the off-medial Shh-expressing cells migrate anteriorly from the diencephalic/mesencephalic junction and start to express Shh once they reach their final position in the hypothalamic primordium (Manning et al., ). It is important to note the expression of Shh is restricted to the ventricular zone, i.e., differentiated neurons or glia cells cease to express Shh. From here on, we will refer to the spatial-temporal classification of Shh expression described above to explain the results of lineage tracing and gene inactivation studies.
Figure 1
Lineage of Shh-expressing and -responding progenitors in the hypothalamus
Shh-expressing cells in the ventral midline (floor plate) of the spinal cord and hindbrain appear to function solely as organizers, i.e., they induce and pattern neighboring precursors but do not contribute progeny to the developing neural tissue (Joksimovic et al., ). In contrast, Shh-expressing cells in the mesencephalic floor plate give rise to neurons (Joksimovic et al., ; Blaess et al., ). To investigate whether Shh-expressing cells contribute to the hypothalamus and whether precursors in the medial vs. lateral Shh-expressing domain give rise to different hypothalamic regions, we used the Shh-CreER mouse line in combination with Cre-inducible reporters for an inducible genetic fate mapping approach (Alvarez-Bolado et al., ; Figures 1F,G). We mapped the distribution of fate-mapped cells in late embryonic and adult brains after tamoxifen administration (TM) (to activate Cre and induce reporter gene expression) at selected stages between E7.5 and E12.5. Based on these data, we were able to characterize the changing lineages that are derived from Shh-expressing cells. The timing of these changes is largely consistent with the early, middle and late patterns of Shh expression. We found that initially (TM at E7.5, corresponding to early pattern), very few cells are derived from Shh-expressing precursors, all of them localized to the posterior hypothalamus. With TM at E8.5 (corresponding to middle pattern), the progeny of Shh-expressing precursors contribute to the mammillary and tuberal regions. At later stages (TM at E9.5, E10.5, E11.5 or E12.5; corresponding to late pattern), Shh-expressing precursors give rise to cells in the preoptic area and in the posterior hypothalamic anlage, but to only few cells in the mammillary nucleus and ventral midline of the tuberal region. Precursors labeled with TM at E12.5 generate relatively few labeled cells and most of these have a morphology indicative of astrocytes. Interestingly, Shh-expressing precursors do not contribute any progeny to the anterior hypothalamic region at any of the labeling stages.
The telencephalic domain of Shh expression corresponds to the preoptic area and the medial ganglionic emminence. Shh-expressing precursors in the medial ganglionic eminence give rise to the globus pallidus (Flandin et al., ; Nóbrega-Pereira et al., ) while the Shh-expressing domain in the preoptic area generates septal neurons (Wei et al., 2012) and preoptic astrocytes (Alvarez-Bolado et al., ). Lineage tracing of the lateral Shh-expressing precursor domain in the chick show that one of the neuronal populations generated from this domain are hypothalamic dopaminergic neurons (Ohyama et al., ).
A detailed characterization of the lineage of Shh-responding (Gli1-expressing) cells in the hypothalamus is lacking. Aoto et al. () used genetic inducible fate mapping with a Gli1CreER line to investigate the lineage of Gli1-expression in craniofacial tissues, but the authors also provide some results on the tuberal hypothalamus. Gli1-expressing precursors labeled with TM at E8.5 give rise to progeny in the mediolateral tuberal hypothalamus (except for the midline). At later stages (TM at E9.5 and E11.5), Gli1-expressing precursors do not contribute to the tuberal hypothalamus. More anterior or posterior areas of the hypothalamus or the fate of Gli1-expressing cells before E8.5 or after E11.5 have not been assessed in this study.
Phenotypes of mouse mutants lacking Shh expression in the forebrain/Function of Shh signaling in the development of the basal hypothalamus
The phenotype of the Shh knock-out mouse mutant was published in 1996 (Chiang et al., ) and famously showed the loss of the ventral portion of the neural tube. The hypothesis that Shh was a key ventralizer, initially based on the Shh expression domain, was confirmed. The loss of ventral midline structures leads to a particularly severe forebrain phenotype: Shh knock-out mice have a single fused telencephalic vesicle and a single fused optic cup (holoprosencephaly) (Chiang et al., ). Studies in chicken uncovered a key role of Shh (together with other determinants like bone morphogenetic proteins (BMPs)) secreted by the prechordal plate in hypothalamic induction and specifically in the specification of the hypothalamic ventral midline (Dale et al., ; Pera and Kessel, ).The dissection between the effects of Shh secreted by the axial mesoderm in the mouse however (notochord and prechordal plate; non-neural Shh) or by the neural tube itself (neural Shh) started more than a decade later. Analysis of Shh knock-out embryos and embryos chimeric for Shh knock-out cells showed that Shh signaling is non-cell autonomously required to maintain the prechordal mesoderm. In the absence of Shh, but also after lesions of the prechordal mesoderm, the forebrain midline does not develop (Aoto et al., ), indicating that Shh signaling from the prechordal mesoderm is essential to induce forebrain midline structures. To investigate the role of Shh secreted from the hypothalamic primordium, several conditional knock-out mouse models were established. Using a Shh floxed allele (Dassule et al., ; Lewis et al., ) and a Foxb1-Cre mouse line (Zhao et al., 2007), which drives Cre expression in the forebrain neuroepithelium starting around E8.0, Szabó et al. () generated a neuroepithelial specific inactivation of Shh in the hypothalamic primordium. Analysis of these conditional Shh mutants showed that neural Shh is required for the specification of the lateral hypothalamus and in particular of the hypocretin/orexin neurons. The medial portion of the basal hypothalamus (tuberal and mammillary regions) was severely reduced in size and showed specification defects (Szabó et al., ).
Nkx2-1 is a specific marker of the hypothalamic primordium starting to be expressed at E8.0 (Shimamura et al., ). Using a Nkx2-1-Cre mouse line (Xu et al., 2008) to inactivate Shh, Shimogori et al. () generated a second conditional mutant mouse model lacking Shh expression in the hypothalamic primordium. The phenotype of these mutants confirmed the requirement of neural Shh for normal hypothalamic growth and for the specification of the basal portion; alterations in lateral hypothalamic markers were also found. In addition, the developmental transcriptome of the mouse hypothalamus was analyzed with microarrays and was used to generate a genomic atlas resource for the study of hypothalamus development (Shimogori et al., ).
Zhao et al. (2012) inactivated Shh in the hypothalamic neuroepithelium using the SBE2-Cre mouse line. SBE2 is a hypothalamus-specific upstream regulatory element of Shh (Jeong et al., ). The results of the mutant analysis confirmed previous results (Szabó et al., ; Shimogori et al., ). Additionally, the study demonstrated that deficiency in neural Shh causes septo-optic dysplasia, a congenital brain anomaly that leads to defects in the pituitary, the optic nerve, and the midline of the forebrain.
The Shh-Gli code in the development of the hypothalamus
Shh signaling is transduced by the two transmembrane receptors Patched (Ptch) and Smoothened and the Gli zinc finger transcription factors (Gli1-3). In the presence of Shh, Gli2 acts as a strong activator in the pathway. Gli3 acts primarily as a repressor of Shh target genes; in presence of Shh the Gli3 repressor function is attenuated and Gli3 can even function as a weak activator. Gli1 (Figures 1E–G, right panels, pink) contributes to the activation of the pathway, but it is only expressed in cells in which Gli2 (or Gli3) activator is already present and, as described above, can be used as a readout for the pathway (Fuccillo et al., ). In the spinal cord, midbrain and telencephalon, the dissection of the relative contribution of Gli activator and repressor function downstream of Shh have given considerable insight into the role of Shh signaling in the specification of these regions. The analysis of this Shh-Gli code in the hypothalamus is still rudimentary. Although Shh is essential for maintenance of prechordal plate and induction of ventral midline (Aoto et al., ), analysis of Gli2 null mutants indicates that Shh expression in the hypothalamus is independent of Shh signaling from underlying prechordal mesoderm (Matise et al., ). In Gli2 knock-out mutants, the bilateral Shh expression domain normally observed after E9.5 (Figure 1G) is fused to a single midline domain in the basal hypothalamus at E11.5, indicating that Gli2-mediated Shh signaling is required for the induction of the ventral midline, but not for the induction of Shh expression in the tuberal hypothalamic neuroepithelium per se (Park et al., ). Nkx2-1 expression is reduced and shifted medially in these mutants. Additional inactivation of Gli1 on the Gli2 knock-out background results in a more severe phenotype; the Nkx2-1 and Shh expression domains are completely missing and the size of the tuberal hypothalamic primordium is severely reduced. Gli1 appears to be primarily important for the partial rescue of Gli2 loss-of-function: mice in which Gli1 is inactivated and which are heterozygous for Gli2 do not have an obvious phenotype in the tuberal hypothalamus (Park et al., ).
Evidence that Gli3 plays a role in hypothalamic development comes from a human malformation syndrome. Pallister-Hall syndrome is associated with several malformations including polydactyly, imperforated anus and hypothalamic hamartomas (a non-cancerous tumor in the tuberal hypothalamus), which develop typically during early gestation (33–41 days) (Clarren et al., ; Hall et al., ). The underlying mutation in the Gli3 gene results in the production of a truncated form of Gli3 protein acting as a constitutive repressor (Meyer and Roelink, ) indicating that the precise regulation of Gli3 repressor levels is essential for normal hypothalamic development in humans. However, mice generated to have this mutation in Gli3 do not develop hypothalamic hamartomas, even though they have most of the other malformations characteristic of the Pallister-Hall syndrome (Böse et al., ). Evidence that Shh-signaling mediated regulation of Gli3 repressor levels is essential for the induction of ventral forebrain structures comes from the analysis of Shh knock-out and Shh/Gli3 double mutant mice: in addition to the loss of ventral midline structures in Shh knock-out mice (Nkx2-1 positive) ventrolateral domains (Dlx2 and Gsh2 positive), are severely reduced. If Gli3 is inactivated in addition to Shh, all three markers are expressed in their normal ventral domains (Rallu et al., ). Whether defects in hypothalamic development in Shh knock-out mice can be rescued by removal of Gli3 has not been investigated. The effects of Gli3 loss-of-function on hypothalamic development in mouse have not been assessed directly, but histological sections through the diencephalon point to the absence of a severe phenotype in Gli3 null mutants (Theil et al., ). In chick, the antagonistic functions of Gli3 activator and repressor have been analyzed by means of their effect on Pax7 expression in the developing hypothalamus. Pax7 is expressed in the lateral hypothalamus, but only at stage 30. Gli3 repressor induces Pax7 expression, while Gli activator inhibits Pax7 expression. Gli3 expression is upregulated by BMP7 and in addition to Gli3 repressor, activation of BMP signaling is necessary for the induction of the Pax7 positive cell fate (Ohyama et al., ).
Neural Shh in the preoptic area and alar hypothalamus
The preoptic area is classically assigned to the hypothalamus, according to its adult functionality, but embryologically it develops from the telencephalon (Puelles et al., ). Both this region and the classical “anterior” (or postoptic) region belong to the alar portion of the forebrain. Shh is expressed in this region of the neural tube as an isolated domain corresponding to the medial ganglionic eminence and preoptic area. These regions generate neurons that migrate to other areas of the telencephalon (Flandin et al., ; Nóbrega-Pereira et al., ; Wei et al., 2012) as well as local preoptic astrocytes (Alvarez-Bolado et al., ). The neural-Shh-specific mutants mentioned above do not show strong phenotypes in the anterior portions of the hypothalamus beyond reduced growth and mild patterning defects (Szabó et al., ; Shimogori et al., ) and the phenotype of these anterior regions has not been investigated in Gli mutant mice (Theil et al., ; Park et al., ). Evidence for an important role of non-neural Shh in the development of the preoptic area was provided by a recent study that identified Lrp2, a member of the LDL receptor gene family, as a component of the Shh signaling pathway important for the induction of the preoptic ventral midline (Christ et al., ). Lrp2 is expressed broadly in the forebrain starting at E7.5, but expression gets restricted to the ventral midline by E9.5. Lrp2 sequesters secreted Shh protein and forms a complex with Ptch that regulates internalization and intracellular trafficking of Shh. In absence of Lrp2, the ventral tissue in the preoptic region fails to respond to Shh secreted from the prechordal mesoderm and medial Shh expression is not induced. Shh expression is shifted to two off-medial domains and Bmp4 and Gli3 are expressed in the ventral midline of the preoptic area in E10.5 Lrp2 knock-out mice. While Shh signaling is obviously required for the development of the preoptic area and alar hypothalamus (see also Pabst et al., ; Rallu et al., ,), we consider that the precise requirements for neural Shh in these regions have been insufficiently analyzed.
Regulation of Shh expression in the hypothalamus
The dynamic expression pattern of Shh during hypothalamic development raises the question on how this pattern is regulated. The transcription factor Six3 regulates Shh expression directly, and haploinsufficiency in Six3 results in the loss of Shh expression in the ventral midline, but only in the alar portion of the hypothalamus (Geng et al., ; Jeong et al., ; Figures 2A,B). Similarly, Sox2 has been shown to bind directly to the SBE2 enhancer and Sox2 and Sox3 have dose dependent effects on Shh expression in the midline of the alar hypothalamus (Zhao et al., 2012). Whether there is a similar transcription factor code required to induce Shh expression in the ventral midline of the basal hypothalamus is unknown, but the mechanisms for the later downregulation of Shh expression in the midline have been investigated. In chick, BMPs act upstream of the transcription factor Tbx2 to inhibit Shh expression in the ventral midline of the basal hypothalamus, allowing this region to acquire its fate as a precursor area (Manning et al., ). In zebrafish, Wnt inhibition induces hypothalamic cell fate (Kapsimali et al., ) and in vitro experiments indicate that, in chick, BMPs act probably by antagonizing Wnt signaling in this region (Manning et al., ). A transcriptional mechanism that integrates Shh and Wnt signaling to regulate ventral specification has been suggested before in the spinal cord (Lei et al., ). In mouse, Tbx3 appears to be required to downregulate Shh in the ventral midline and both Tbx2 and Tbx3 have the ability to suppress Shh expression by sequestering the transcription factor Sox2 away from the SBE2 forebrain enhancer (Trowe et al., 2013).
Figure 2
Conclusion
Ever since the first Shh knockout mouse (Chiang et al., ), our knowledge of the precise roles of Shh, prechordal or neuroepithelial, on hypothalamic specification and growth has steadily increased. Experimental approaches in chick and the phenotypical analysis of a variety of zebrafish and mouse mutants have demonstrated that prechordal Shh is necessary for midline specification, while neuroepithelial Shh plays a role in hypothalamic growth and in the specification of the lateral hypothalamic area. In addition to the functional insights, lineage tracing of neuroepithelial Shh in the hypothalamus has provided further important clues on hypothalamic development. Areas still in need of work are among others the specific Shh-Gli code for the different hypothalamic regions as well as elucidating the role of Shh on the alar hypothalamus (anterior hypothalamic region) and the preoptic area (a telencephalic area functionally included in the adult hypothalamus).
Statements
Conflict of interest
The Guest Associate Editor Valery Grinevich declares that, despite being affiliated to the same institution as authors Roberta Haddad-Tóvolli, Xunlei Zhou and Gonzalo Álvarez-Bolado, the review process was handled objectively and no conflict of interest exists. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
1
Alvarez-BoladoG.PaulF. A.BlaessS. (2012). Sonic hedgehog lineage in the mouse hypothalamus: from progenitor domains to hypothalamic regions. Neural Dev.7:4. 10.1186/1749-8104-7-4
2
AotoK.ShikataY.ImaiH.MatsumaruD.TokunagaT.ShiodaS.et al. (2009). Mouse Shh is required for prechordal plate maintenance during brain and craniofacial morphogenesis. Dev. Biol.327, 106–120. 10.1016/j.ydbio.2008.11.022
3
BarthK. A.WilsonS. W. (1995). Expression of zebrafish nk2.2 is influenced by sonic hedgehog/vertebrate hedgehog-1 and demarcates a zone of neuronal differentiation in the embryonic forebrain. Development121, 1755–1768.
4
BlaessS.BodeaG. O.KabanovaA.ChanetS.MugnieryE.DerouicheA.et al. (2011). Temporal-spatial changes in Sonic hedgehog expression and signaling reveal different potentials of ventral mesencephalic progenitors to populate distinct ventral midbrain nuclei. Neural Dev.6:29. 10.1186/1749-8104-6-29
5
BöseJ.GrotewoldL.RütherU. (2002). Pallister-Hall syndrome phenotype in mice mutant for Gli3. Hum. Mol. Genet.11, 1129–1135. 10.1093/hmg/11.9.1129
6
ChangD. T.LópezA.von KesslerD. P.ChiangC.SimandlB. K.ZhaoR.et al. (1994). Products, genetic linkage and limb patterning activity of a murine hedgehog gene. Development120, 3339–3353.
7
ChiangC.LitingtungY.LeeE.YoungK. E.CordenJ. L.WestphalH.et al. (1996). Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function. Nature383, 407–413. 10.1038/383407a0
8
ChristA.ChristaA.KurE.LioubinskiO.BachmannS.WillnowT. E.et al. (2012). LRP2 is an auxiliary SHH receptor required to condition the forebrain ventral midline for inductive signals. Dev. Cell22, 268–278. 10.1016/j.devcel.2011.11.023
9
ClarrenS. K.AlvordE. C.Jr.HallJ. G. (1980). Congenital hypothalamic hamartoblastoma, hypopituitarism, imperforate anus and postaxial polydactyly–a new syndrome? Part II: neuropathological considerations. Am. J. Med. Genet.7, 75–83. 10.1002/ajmg.1320070111
10
DaleJ. K.VesqueC.LintsT. J.SampathT. K.FurleyA.DoddJ.et al. (1997). Cooperation of BMP7 and SHH in the induction of forebrain ventral midline cells by prechordal mesoderm. Cell90, 257–269. 10.1016/s0092-8674(00)80334-7
11
DassuleH. R.LewisP.BeiM.MaasR.McMahonA. P. (2000). Sonic hedgehog regulates growth and morphogenesis of the tooth. Development127, 4775–4785.
12
EchelardY.EpsteinD. J.St-JacquesB.ShenL.MohlerJ.McMahonJ. A.et al. (1993). Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity. Cell75, 1417–1430. 10.1016/0092-8674(93)90627-3
13
FlandinP.KimuraS.RubensteinJ. L. (2010). The progenitor zone of the ventral medial ganglionic eminence requires Nkx2–1 to generate most of the globus pallidus but few neocortical interneurons. J. Neurosci.30, 2812–2823. 10.1523/JNEUROSCI.4228-09.2010
14
FuccilloM.JoynerA. L.FishellG. (2006). Morphogen to mitogen: the multiple roles of hedgehog signalling in vertebrate neural development. Nat. Rev. Neurosci.7, 772–783. 10.1038/nrn1990
15
FurimskyM.WallaceV. A. (2006). Complementary Gli activity mediates early patterning of the mouse visual system. Dev. Dyn.235, 594–605. 10.1002/dvdy.20658
16
GengX.SpeirsC.LagutinO.InbalA.LiuW.Solnica-KrezelL.et al. (2008). Haploinsufficiency of Six3 fails to activate Sonic hedgehog expression in the ventral forebrain and causes holoprosencephaly. Dev. Cell15, 236–247. 10.1016/j.devcel.2008.07.003
17
GoodrichL. V.JohnsonR. L.MilenkovicL.McMahonJ. A.ScottM. P. (1996). Conservation of the hedgehog/patched signaling pathway from flies to mice: induction of a mouse patched gene by hedgehog. Genes Dev.10, 301–312. 10.1101/gad.10.3.301
18
HallJ. G.PallisterP. D.ClarrenS. K.BeckwithJ. B.WiglesworthF. W.FraserF. C.et al. (1980). Congenital hypothalamic hamartoblastoma, hypopituitarism, imperforate anus and postaxial polydactyly–a new syndrome? Part I: clinical, causal and pathogenetic considerations. Am. J. Med. Genet.7, 47–74. 10.1002/ajmg.1320070110
19
HuiC. C.SlusarskiD.PlattK. A.HolmgrenR.JoynerA. L. (1994). Expression of three mouse homologs of the Drosophila segment polarity gene cubitus interruptus, Gli, Gli-2 and Gli-3, in ectoderm- and mesoderm-derived tissues suggests multiple roles during postimplantation development. Dev. Biol.162, 402–413. 10.1006/dbio.1994.1097
20
JeongY.El-JaickK.RoesslerE.MuenkeM.EpsteinD. J. (2006). A functional screen for sonic hedgehog regulatory elements across a 1 Mb interval identifies long-range ventral forebrain enhancers. Development133, 761–772. 10.1242/dev.02239
21
JeongY.LeskowF. C.El-JaickK.RoesslerE.MuenkeM.YocumA.et al. (2008). Regulation of a remote Shh forebrain enhancer by the Six3 homeoprotein. Nat. Genet.40, 1348–1353. 10.1038/ng.230
22
JoksimovicM.AndereggA.RoyA.CampochiaroL.YunB.KittappaR.et al. (2009). Spatiotemporally separable Shh domains in the midbrain define distinct dopaminergic progenitor pools. Proc. Natl. Acad. Sci. U S A106, 19185–19190. 10.1073/pnas.0904285106
23
KapsimaliM.CaneparoL.HouartC.WilsonS. W. (2004). Inhibition of Wnt/Axin/beta-catenin pathway activity promotes ventral CNS midline tissue to adopt hypothalamic rather than floorplate identity. Development131, 5923–5933. 10.1242/dev.01453
24
LeeJ.PlattK. A.CensulloP.Ruiz i AltabaA. (1997). Gli1 is a target of Sonic hedgehog that induces ventral neural tube development. Development124, 2537–2552.
25
LeiQ.JeongY.MisraK.LiS.ZelmanA. K.EpsteinD. J.et al. (2006). Wnt signaling inhibitors regulate the transcriptional response to morphogenetic Shh-Gli signaling in the neural tube. Dev. Cell11, 325–337. 10.1016/j.devcel.2006.06.013
26
LewisP. M.Gritli-LindeA.SmeyneR.KottmannA.McMahonA. P. (2004). Sonic hedgehog signaling is required for expansion of granule neuron precursors and patterning of the mouse cerebellum. Dev. Biol.270, 393–410. 10.1016/j.ydbio.2004.03.007
27
ManningL.OhyamaK.SaegerB.HatanoO.WilsonS. A.LoganM.et al. (2006). Regional morphogenesis in the hypothalamus: a BMP-Tbx2 pathway coordinates fate and proliferation through Shh downregulation. Dev. Cell11, 873–885. 10.1016/j.devcel.2006.09.021
28
MarigoV.JohnsonR. L.VortkampA.TabinC. J. (1996). Sonic hedgehog differentially regulates expression of GLI and GLI3 during limb development. Dev. Biol.180, 273–283. 10.1006/dbio.1996.0300
29
MarigoV.TabinC. J. (1996). Regulation of patched by sonic hedgehog in the developing neural tube. Proc. Natl. Acad. Sci. U S A93, 9346–9351. 10.1073/pnas.93.18.9346
30
MathieuJ.BarthA.RosaF. M.WilsonS. W.PeyriérasN. (2002). Distinct and cooperative roles for Nodal and hedgehog signals during hypothalamic development. Development129, 3055–3065.
31
MatiseM. P.EpsteinD. J.ParkH. L.PlattK. A.JoynerA. L. (1998). Gli2 is required for induction of floor plate and adjacent cells, but not most ventral neurons in the mouse central nervous system. Development125, 2759–2770.
32
MeyerN. P.RoelinkH. (2003). The amino-terminal region of Gli3 antagonizes the Shh response and acts in dorsoventral fate specification in the developing spinal cord. Dev. Biol.257, 343–355. 10.1016/s0012-1606(03)00065-4
33
Nóbrega-PereiraS.GelmanD.BartoliniG.PlaR.PieraniA.MarínO. (2010). Origin and molecular specification of globus pallidus neurons. J. Neurosci.30, 2824–2834. 10.1523/JNEUROSCI.4023-09.2010
34
OhyamaK.DasR.PlaczekM. (2008). Temporal progression of hypothalamic patterning by a dual action of BMP. Development135, 3325–3331. 10.1242/dev.027078
35
OhyamaK.EllisP.KimuraS.PlaczekM. (2005). Directed differentiation of neural cells to hypothalamic dopaminergic neurons. Development132, 5185–5197. 10.1242/dev.02094
36
OhyamaK.IkedaE.KawamuraK.MaedaN.NodaM. (2004). Receptor-like protein tyrosine phosphatase zeta/RPTP beta is expressed on tangentially aligned neurons in early mouse neocortex. Brain Res. Dev. Brain Res.148, 121–127. 10.1016/j.devbrainres.2003.10.012
37
PabstO.HerbrandH.TakumaN.ArnoldH. H. (2000). NKX2 gene expression in neuroectoderm but not in mesendodermally derived structures depends on sonic hedgehog in mouse embryos. Dev. Genes Evol.210, 47–50. 10.1007/pl00008188
38
ParkH. L.BaiC.PlattK. A.MatiseM. P.BeeghlyA.HuiC. C.et al. (2000). Mouse Gli1 mutants are viable but have defects in SHH signaling in combination with a Gli2 mutation. Development127, 1593–1605.
39
PeraE. M.KesselM. (1997). Patterning of the chick forebrain anlage by the prechordal plate. Development124, 4153–4162.
40
PlaczekM.BriscoeJ. (2005). The floor plate: multiple cells, multiple signals. Nat. Rev. Neurosci.6, 230–240. 10.1038/nrn1628
41
PlattK. A.MichaudJ.JoynerA. L. (1997). Expression of the mouse Gli and Ptc genes is adjacent to embryonic sources of hedgehog signals suggesting a conservation of pathways between flies and mice. Mech. Dev.62, 121–135. 10.1016/s0925-4773(96)00648-x
42
PuellesL.Martinez-de-la-TorreM.BardetS.RubensteinJ. L. R. (2012). “Hypothalamus,” in The Mouse Nervous System, eds WatsonC.PaxinosG.PuellesL. (San Diego: Elsevier-Academic Press), 221–312.
43
RalluM.CorbinJ. G.FishellG. (2002a). Parsing the prosencephalon. Nat. Rev. Neurosci.3, 943–951. 10.1038/nrn989
44
RalluM.MacholdR.GaianoN.CorbinJ. G.McMahonA. P.FishellG. (2002b). Dorsoventral patterning is established in the telencephalon of mutants lacking both Gli3 and hedgehog signaling. Development129, 4963–4974.
45
ShimamuraK.HartiganD. J.MartinezS.PuellesL.RubensteinJ. L. (1995). Longitudinal organization of the anterior neural plate and neural tube. Development121, 3923–3933.
46
ShimogoriT.LeeD. A.Miranda-AnguloA.YangY.WangH.JiangL.et al. (2010). A genomic atlas of mouse hypothalamic development. Nat. Neurosci.13, 767–775. 10.1038/nn.2545
47
SzabóN. E.ZhaoT.CankayaM.TheilT.ZhouX.Alvarez-BoladoG. (2009). Role of neuroepithelial Sonic hedgehog in hypothalamic patterning. J. Neurosci.29, 6989–7002. 10.1523/jneurosci.1089-09.2009
48
TheilT.Alvarez-BoladoG.WalterA.RütherU. (1999). Gli3 is required for Emx gene expression during dorsal telencephalon development. Development126, 3561–3571.
49
TroweM. O.ZhaoL.WeissA. C.ChristoffelsV.EpsteinD. J.KispertA. (2013). Inhibition of Sox2-dependent activation of Shh in the ventral diencephalon by Tbx3 is required for formation of the neurohypophysis. Development140, 2299–2309. 10.1242/dev.094524
50
WeiB.HuangZ.HeS.SunC.YouY.LiuF.et al. (2012). The onion skin-like organization of the septum arises from multiple embryonic origins to form multiple adult neuronal fates. Neuroscience222, 110–123. 10.1016/j.neuroscience.2012.07.016
51
XuQ.TamM.AndersonS. A. (2008). Fate mapping Nkx2.1-lineage cells in the mouse telencephalon. J. Comp. Neurol.506, 16–29. 10.1002/cne.21529
52
ZhaoL.ZevallosS. E.RizzotiK.JeongY.Lovell-BadgeR.EpsteinD. J. (2012). Disruption of SoxB1-dependent Sonic hedgehog expression in the hypothalamus causes septo-optic dysplasia. Dev. Cell22, 585–596. 10.1016/j.devcel.2011.12.023
53
ZhaoT.ZhouX.SzabóN.LeitgesM.Alvarez-BoladoG. (2007). Foxb1-driven Cre expression in somites and the neuroepithelium of diencephalon, brainstem and spinal cord. Genesis45, 781–787. 10.1002/dvg.20356
Summary
Keywords
chick, development, Gli, hypothalamus, lineage, mouse, phenotype, Shh
Citation
Blaess S, Szabó N, Haddad-Tóvolli R, Zhou X and Álvarez-Bolado G (2015) Sonic hedgehog signaling in the development of the mouse hypothalamus. Front. Neuroanat. 8:156. doi: 10.3389/fnana.2014.00156
Received
31 October 2014
Accepted
02 December 2014
Published
06 January 2015
Volume
8 - 2014
Edited by
Valery Grinevich, German Cancer Research Center DKFZ and University of Heidelberg, Germany
Reviewed by
Sara Ferrando, University of Genoa, Italy; Clemens Martin Kiecker, King’s College London, UK
Copyright
© 2015 Blaess, Szabó, Haddad-Tóvolli, Zhou and Álvarez-Bolado.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Sandra Blaess, Neurodevelopmental Genetics, Institute of Reconstructive Neurobiology, University of Bonn, Sigmund-Freud-Straße 25, D-53127 Bonn, Germany e-mail: sandra.blaess@uni-bonn.de; Gonzalo Álvarez-Bolado, Department of Medical Cell Biology, Institute of Anatomy and Cell Biology, University of Heidelberg, Im Neuenheimer Feld 307, D-69120 Heidelberg, Germany e-mail: alvarez@ana.uni-heidelberg.de
This article was submitted to the journal Frontiers in Neuroanatomy.
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