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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2018.00552

Expanded Somatic Mutation Spectrum of MED12 Gene in Uterine Leiomyomas of Saudi Arabian Women

Ghada M. Ajabnoor1, Nesma A. Mohammed1,  Babajan Banganapalli1, Layla S. Abdullah1, Ola N. Bondagji1, Nisma Mansouri1, Nora N. Al-Sahly1,  Venkatesh Vaidyanathan2, Nabeel Bondagji1,  Ramu Elango1 and  Noor A. Shaik1*
  • 1King Abdulaziz University, Saudi Arabia
  • 2University of Auckland, New Zealand

MED12, a subunit of mediator complex genes is known to harbor genetic mutations, mostly in exon 2, causal to the genesis of uterine leiomyomas among Caucasian, African American and Asian women. However, the precise relationship between genetic mutations versus protein or disease phenotype is not well explained. Therefore, we sought to replicate the MED12 mutation frequency in leiomyomas of Saudi Arabian women, who represents ethnically and culturally distinct population. We performed molecular screening of MED12 gene (in 308 chromosomes belonging to 154 uterine biopsies), analyzed the genotype-disease phenotype correlations and determined the biophysical characteristics of mutated protein through diverse computational approaches. We discovered that >44% (34/77) leiomyomas of Arab women carry a spectrum of MED12 mutations (30 missense, 1 splice site and 3 indels). In addition to known codon 44, we observed novel somatic mutations in codons 36, 38 and 55. Majority of MED12 mutated tumors 27/30; 90%) showed only one type of genetic change, asserting their deleterious effect on protein function. An interesting inverse correlation between tumor size and LH is observed when tumor is positive to MED12 mutation (p<0.05). Our computational investigations suggest that amino acid substitution mutations in exon-2 region of MED12 might contribute to potential alterations in phenotype as well as the stability of MED12 protein. Our study, being the first one from Arab world, confirms the previous findings that somatic MED12 mutations are critical to development and progression of uterine leiomyomas irrespective of the ethnic background. We recommend that mutation screening, particularly codon 44 of MED12 can assist in molecular diagnostics of uterine leiomyomas in majority of the patients.

Keywords: Cancer, Exome, Uterine leiomyomas (UL), Arab Women, MED12

Received: 21 Jul 2018; Accepted: 29 Oct 2018.

Edited by:

Haranatha R. Potteti, University of Illinois at Chicago, United States

Reviewed by:

Sambasivan Venkatasubramanian, University of Washington, United States
R.Krishna Kishore, University of Agricultural Sciences, Bangalore, India  

Copyright: © 2018 Ajabnoor, Mohammed, Banganapalli, Abdullah, Bondagji, Mansouri, Al-Sahly, Vaidyanathan, Bondagji, Elango and Shaik. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Noor A. Shaik, King Abdulaziz University, Jeddah, Saudi Arabia, noorahmadh@gmail.com