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Review ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2018.00562

Generation of 2′,3′-cyclic phosphate-containing RNAs as a hidden layer of the transcriptome

Megumi Shigematsu1, Takuya Kawamura1 and  Yohei Kirino1*
  • 1Thomas Jefferson University, United States

Cellular RNA molecules contain phosphate or hydroxyl ends. A 2′,3′-cyclic phosphate (cP) is one of the 3′-terminal forms of RNAs mainly generated from RNA cleavage by ribonucleases. Although transcriptome profiling using RNA-seq has become a ubiquitous tool in biological and medical research, cP-containing RNAs (cPRs) form a hidden transcriptome layer, which is infrequently recognized and characterized, because standard RNA-seq is unable to capture them. Despite cPRs’ invisibility in RNA-seq data, increasing evidence indicates that they are not accumulated simply as non-functional degradation products; rather, they have physiological roles in various biological processes, designating them as noteworthy functional molecules. This review summarizes our current knowledge of cPR biogenesis pathways and their catalytic enzymatic activities, discusses how the cPR generation affects biological processes, and explores future directions to further investigate cPR biology.

Keywords: 2′,3′-cyclic phosphate (cP), cP-containing RNA (cPR), cP-RNA-seq, Ribonuclease, tRNA half, Non-coding RNA (ncRNA), angiogenin (ANG)

Received: 28 Sep 2018; Accepted: 06 Nov 2018.

Edited by:

Akio Kanai, Institute for Advanced Biosciences, Keio University, Japan

Reviewed by:

Gota Kawai, Chiba Institute of Technology, Japan
Kiong Ho, Faculty of Medicine, University of Tsukuba, Japan
Stefan Weitzer, Medical University of Vienna, Austria  

Copyright: © 2018 Shigematsu, Kawamura and Kirino. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Yohei Kirino, Thomas Jefferson University, Philadelphia, 19107, Pennsylvania, United States, Yohei.Kirino@jefferson.edu