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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2019.00149

The neuronal Gclc overexpression in Drosophila melanogaster reverses aging-related transcriptomic changes in thorax

  • 1Radiation ecology, Institute of Biology, Komi Scientific Center (RAS), Russia
  • 2Engelhardt Institute of Molecular Biology (RAS), Russia
  • 3Moscow Institute of Physics and Technology, Russia
  • 4Insilico Medicine, Inc., United States

Some effects of aging in animals are tissue-specific. In D. melanogaster neuronal overexpression of Gclc increases lifespan and improves certain physiological parameters associated with health benefits such as locomotor activity, circadian rhythmicity and stress resistance. Our previous transcriptomic analyses of Drosophila heads, that are primarily composed of neuronal tissue, revealed the significant changes in expression levels of genes involved in aging-related signaling pathways (Jak-STAT, MAPK, FOXO, Notch, mTOR, TGF-beta), translation, protein processing in endoplasmic reticulum, proteasomal degradation, glycolysis, oxidative phosphorylation, apoptosis, regulation of circadian rhythms, differentiation of neurons, synaptic plasticity and transmission. One of the major diseases of aging is sarcopenia. The purpose of the present study was to investigate the effects of the neuronal Gclc overexpression on gene expression levels in imago thorax, which is primarily composed of muscles. А total of 58 genes were found to be differentially expressed between thoraces of control and Gclc overexpressing flies. Among the functional categories of differentially expressed genes that were overrepresented were Drug metabolism, Metabolism of xenobiotics by cytochrome P450, Glutathione metabolism, Starch and sucrose metabolism, Neuroactive ligand-receptor interaction, One carbon pool by folate, vesicle, Cdc73/Paf1 complex.

Keywords: GCLC (glutamate-cysteine ligase catalytic subunit, Glutathione, Lifespan - Longevity, Gene Expression, Drosophila melanogaster

Received: 25 Jun 2018; Accepted: 12 Feb 2019.

Edited by:

Elena G. Pasyukova, Institute of Molecular Genetics (RAS), Russia

Reviewed by:

Kyung-Jin Min, Inha University, South Korea
John Tower, University of Southern California, United States  

Copyright: © 2019 Moskalev, Guvatova, Shaposhnikov, Lashmanova, Proshkina, Koval, Zhavoronkov, Krasnov and Kudryavtseva. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Alexey Moskalev, Institute of Biology, Komi Scientific Center (RAS), Radiation ecology, Syktyvkar, 167982, Russia, amoskalev@list.ru