Original Research ARTICLE
Downregulation of Long Non-Coding RNA FALEC Inhibits Gastric Cancer Cell Migration and Invasion Through Impairing ECM1 Expression by Exerting its Enhancer-like Function
- 1Bengbu Medical College, China
- 2Nanjing Maternity and Child Health Care Hospital, China
- 3School of Public Health, Bengbu Medical College, China
- 4First Affiliated Hospital of Bengbu Medical College, China
- 5Wannan Medical College, China
Long non-coding RNAs (lncRNAs) have been shown to play important roles in many human diseases. However, their functions and mechanisms in tumorigenesis and development remain largely unknown. Here, we demonstrated that focally amplified long non-coding RNA in epithelial cancer (FALEC) was upregulated and significantly correlated with lymph node metastasis, TNM stage in gastric cancer (GC). Further experiments revealed that FALEC knockdown significantly inhibited GC cells migration and invasion in vitro. Mechanistic investigations demonstrated that small interfering RNA-induced silencing of FALEC decreased expression of the nearby gene extracellular matrix protein 1 (ECM1) in cis. Additionally, ECM1 and FALEC expression were positively correlated, and high levels of ECM1 predicted shorter survival time in GC patients. Our results suggest that the downregulation of FALEC significantly inhibited the migration and invasion of GC cells through impairing ECM1 expression by exerting an enhancer-like function. Our work provides valuable information and a novel promising target for developing new therapeutic strategies in GC.
Keywords: lncRNA (long non-coding RNA), FALEC, ECM1, migration and invasion, gastric cancer
Received: 10 Sep 2018;
Accepted: 07 Mar 2019.
Edited by:Heather Cunliffe, University of Otago, New Zealand
Reviewed by:César López-Camarillo, Universidad Autónoma de la Ciudad de México, Mexico
Sarah D. Diermeier, University of Otago, New Zealand
Copyright: © 2019 Wu, Qiao, Zhao, Wu, Hu, Wu, Huang, Chen, Sun, Liu and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Prof. Mulin Liu, First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui Province, China, firstname.lastname@example.org
Mr. Jinsong Zhao, Wannan Medical College, Wuhu, Anhui Province, China, email@example.com